A5040578491- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Chromosomal and Genetic Variations
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Genetics and Neurodevelopmental Disorders
- Gene expression and cancer classification
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Protein Degradation and Inhibitors
- Fungal and yeast genetics research
- Gene Regulatory Network Analysis
- Plant Virus Research Studies
- RNA regulation and disease
- Advanced NMR Techniques and Applications
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
Centre National de la Recherche Scientifique
2010-2023
Institut Jacques Monod
2014-2023
Université Paris Cité
2014-2023
Fondation ARC pour la Recherche sur le Cancer
2018
Délégation Paris 7
2010
Sorbonne Université
2008-2009
École Normale Supérieure - PSL
2006
National Institute of Diabetes and Digestive and Kidney Diseases
2004
National Institutes of Health
2004
École Normale Supérieure
2003
To get insights into the regulation of replication initiation, we systematically mapped origins along 1% human genome in HeLa cells. We identified 283 origins, 10 times more than previously known. Origin density is strongly correlated with genomic landscapes, clusters closely spaced GC-rich regions and no large GC-poor regions. sequences are evolutionarily conserved, half them map within or near CpG islands. Most overlap transcriptional regulatory elements, providing further evidence a...
The duplication of mammalian genomes is under the control a spatiotemporal program that orchestrates positioning and timing firing replication origins. molecular mechanisms coordinating activation about predicted origins remain poorly understood, partly due to intrinsic rarity bubbles, making it difficult purify short nascent strands (SNS). precise identification based on high-throughput sequencing SNS constitutes new methodological challenge. We propose statistical method with controlled...
Article4 September 2014Open Access Determinants of G quadruplex-induced epigenetic instability in REV1-deficient cells Davide Schiavone Medical Research Council Laboratory Molecular Biology, Cambridge, UK Search for more papers by this author Guillaume Guilbaud Pierre Murat Department Chemistry, University Charikleia Papadopoulou Peter Sarkies The Gurdon Institute, Marie-Noëlle Prioleau Institut Jacques Monod, Université Paris Diderot-Paris 7, Paris, France Shankar Balasubramanian Julian E...
The nuclear genomes of vertebrates show a highly organized program DNA replication where GC-rich isochores are replicated early in S-phase, while AT-rich late replicating. regions gene dense and enriched for active transcription, suggesting connection between regulation timing. Insulator elements can organize independent domains transcription suitable candidates being key regulators We have tested the impact inserting strong origin flanked by β-globin HS4 insulator on timing naturally...
Replication of vertebrate genomes is tightly regulated to ensure accurate duplication, but our understanding the interplay between genetic and epigenetic factors in this regulation remains incomplete. Here, we investigated involvement three elements enriched at gene promoters replication origins: guanine-rich motifs potentially forming G-quadruplexes (pG4s), nucleosome-free regions (NFRs), histone variant H2A.Z, firing origins vertebrates. We show that two pG4s on same DNA strand (dimeric...
Assessment of the impact DNA replication on genome architecture in Eukaryotes has long been hampered by scarcity experimental data. Recent work, relying computational predictions origins replication, suggested that might be a major determinant gene organization human (Huvet et al. 2007. Human driven coordination and transcription. Genome Res. 17:1278–1285). Here, we address this question analyzing first large-scale data set experimentally determined human: 283 identified HeLa cells, 1%...
Chromatin structure is believed to exert a strong effect on replication origin function. We have studied the of chicken β-globin locus, whose chromatin has been extensively characterized. This locus delimited by hypersensitive sites (HSs) that mark position insulator elements. A stretch condensed and another HS separate domain from an adjacent folate receptor (FR) gene. demonstrate here in erythroid cells express FR but not globin genes, initiates at four within domain, one 5′ HS4 other...
Abstract Histone post-translational modifications promote a chromatin environment that controls transcription, DNA replication and repair, but surprisingly few phosphorylations have been documented. We report the discovery of histone H3 serine-57 phosphorylation (H3S57ph) show it is implicated in different repair pathways from fungi to vertebrates. identified CHK1 as major human H3S57 kinase, disrupting or constitutively mimicking H3S57ph had opposing effects on rate recovery stress, 53BP1...
Eukaryotic genomes are replicated under the control of a highly sophisticated program during restricted time period corresponding to S phase. The most widely used replication timing assays, which performed on populations millions cells, suggest that genome is synchronously homologous chromosomes. We investigated stochastic nature this temporal program, by comparing precise times allelic loci within single vertebrate cells progressing through phase at six from very early late. show strictly...
ORI sequences. A few months ago, common features shared by large groups of ORIs were extracted from a large-scale mapping replication origins based on the resistance short nascent strands (SNS) to l-exonuclease digestion [5]. This study, conducted in HeLa cells and covering approximately 1% human genome, showed that origin density is strongly correlated with genomic landscapes, clusters closely spaced GC-rich regions no or only GC-poor regions. Moreover, half mapped within near CpG islands....
The replication program of vertebrate genomes is driven by the chromosomal distribution and timing activation tens thousands origins. Genome-wide studies have shown association origins with promoters CpG islands, their enrichment in G-quadruplex motifs (G4). However, genetic determinants driving activity remain poorly understood. To gain insight on constraints operating origins, we conducted first evolutionary comparison across vertebrates. We generated a genome-wide map chicken (the bird...