A5011322198- Fungal and yeast genetics research
- Prion Diseases and Protein Misfolding
- Genomics and Chromatin Dynamics
- Neurological diseases and metabolism
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Trace Elements in Health
- DNA Repair Mechanisms
- Biofuel production and bioconversion
- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Amino Acid Enzymes and Metabolism
- RNA and protein synthesis mechanisms
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Biochemical Acid Research Studies
- Plant Disease Resistance and Genetics
- Genetics, Aging, and Longevity in Model Organisms
- Prenatal Screening and Diagnostics
- Click Chemistry and Applications
- Plant Reproductive Biology
- Fermentation and Sensory Analysis
- Plant-Microbe Interactions and Immunity
- Pancreatic function and diabetes
Centre National de la Recherche Scientifique
2004-2023
Université de Montpellier
2017-2023
Inserm
2023
Institut de Génétique Moléculaire de Montpellier
2018-2022
Institut de Biochimie et Génétique Cellulaires
2003-2018
University of Fribourg
2008-2013
Dana-Farber Cancer Institute
2005-2011
Harvard University
2006-2011
Université de Bordeaux
2007
The TORC1 and PKA protein kinases are central elements of signaling networks that regulate eukaryotic cell proliferation in response to growth factors and/or nutrients. In yeast, attenuation by these following nitrogen carbon limitation activates the kinase Rim15, which orchestrates initiation a reversible cellular quiescence program ensure normal chronological life span. molecular linking Rim15 distal readouts including expression Msn2/4- Gis1-dependent genes involve endosulfines Igo1/2....
Background6-Aminophenanthridine (6AP) and Guanabenz (GA, a drug currently in use for the treatment of hypertension) were isolated as antiprion drugs using yeast-based assay. These structurally unrelated molecules are also active against mammalian prion several cell-based assays vivo mouse model prion-based diseases.Methodology/Principal FindingsHere we report identification cellular targets these drugs. Using affinity chromatography matrices both drugs, demonstrate an RNA-dependent...
How cells coordinate growth and division is key for size homeostasis. Phosphorylation by G1-CDK of Whi5/Rb inhibitors SBF/E2F transcription factors triggers irreversible S-phase entry in yeast metazoans, but why this occurs at a given cell not fully understood. We show that the Rim15-Igo1,2 pathway, orthologous to Gwl-Arpp19/ENSA, up-regulated early G1 helps promoting START preventing PP2A Cdc55 dephosphorylate Whi5. RIM15 overexpression lowers while IGO1,2 deletion delays with low CDK...
The conserved mitotic kinase Bub1 performs multiple functions that are only partially characterized. Besides its role in the spindle assembly checkpoint and chromosome alignment, is crucial for kinetochore recruitment of proteins, among them Sgo1. Both Sgo1 dispensable growth haploid diploid budding yeast, but they become essential cells with higher ploidy. We find overexpression SGO1 corrects segregation defect bub1Δ restores viability to tetraploid cells. Using an unbiased high-copy...
Growth factors and essential nutrients are key controllers of eukaryotic cell proliferation. In their absence, cells may enter into a quiescent (G0) state. yeast, nitrogen and/or carbon limitation causes downregulation the conserved TORC1 PKA signaling pathways consequently activation Rim15, member PAS protein kinase family. Rim15 orchestrates initiation G0 program in part by coordinating transcription Msn2/4- Gis1-dependent genes with posttranscriptional protection corresponding mRNAs via...
The [URE3] prion of Saccharomyces cerevisiae is a self-propagating inactive form the nitrogen catabolism regulator Ure2p. To determine whether conserved in S. cerevisiae-related yeast species, we have developed genetic tools allowing detection paradoxus and uvarum. We found that In contrast, was not detected paradoxus. inability Ure2p to switch isoform results from primary sequence protein lack cellular cofactors as heterologous can propagate this species. Our data therefore demonstrate only...
Abstract Histone post-translational modifications promote a chromatin environment that controls transcription, DNA replication and repair, but surprisingly few phosphorylations have been documented. We report the discovery of histone H3 serine-57 phosphorylation (H3S57ph) show it is implicated in different repair pathways from fungi to vertebrates. identified CHK1 as major human H3S57 kinase, disrupting or constitutively mimicking H3S57ph had opposing effects on rate recovery stress, 53BP1...
We examined aspects of the URE2/[URE3] prion system in Kluyveromyces lactis, which lies on a different evolutionary branch from Saccharomyces. first analysed polymorphism prion-forming domain 38 strains. Considerable differences were found between these two genera, with little variation within K. lactis. then regulatory function Ure2p, using deletion URE2. assessed deregulation reporter genes: DAL5 and GDH2. Both derepressed mutant strain, as Finally, we tried to obtain [URE3] Despite use...
Aß metabolism plays a pivotal role in Alzheimer's disease. Here, we used yeast model to monitor Aß42 toxicity when entering the secretory pathway and demonstrate that processing in, exit from endoplasmic reticulum (ER) is required unleash full toxic potential. Consistent with previously reported data, our data suggests interacts mitochondria, thereby enhancing formation of reactive oxygen species eventually leading cell demise. We search for genes modulate this deleterious effect, either by...
Eukaryotic cells rapidly adjust the levels of mRNAs in response to environmental stress primarily by controlling transcription and mRNA turnover. How different conditions influence fate stress-responsive mRNAs, however, is relatively poorly understood. This largely due fact that half-life assays are traditionally based on interventions (e.g., temperature-shifts using temperature-sensitive RNA polymerase II alleles or treatment with general inhibitory drugs), which, rather than blocking,...
For years, Saccharomyces cerevisiae has been used as a model organism to gain insight into complex biological processes. The study of closely related yeast species may be critical for understanding the molecular mechanism evolution. Among those species, S. bayanus var. uvarum could particularly pertinent because availability its genome sequence. However, date, in that genetic studies are problematical due lack standard strains collection and methods. Here, we have developed heterothallic...
The BAR proteins are a well-conserved family of including Rvsp in yeast, amphiphysins and Bin mammals. In as mammals, known to be implicated vesicular traffic. Gyp5p (Ypl249p) Ymr192p interact two-hybrid tests with both Rvs161p Rvs167p. is Ypt/Rab-specific GAP highly similar Gyp5p. To specify the interaction between Gyp5p, we used determine domains necessary for these interactions. specific SH3 domain Rvs167p interacted N-terminal Moreover, could form homodimer. Fus2 protein partner tests....