Abstract The present series of experiments investigated the effect a reducing environment created by addition reduced glutathione (GSH) or thioredoxin (TRX) to in vitro culture medium on developmental competence produced porcine embryos, and their intracellular redox status. Porcine cumulus–oocyte complexes were collected from ovaries matured fertilized vitro. putative zygotes then cultured for 6 days modified NCSU‐37 with without (control) GSH TRX, was evaluated. In addition, status embryos...

Functional genes and proteins in sperm play an essential role bulls’ reproductive processes. They are more accurate determining bull fertility than conventional semen quality tests. Protamine-1 (PRM1) is a gene or protein crucial for packaging protecting DNA until fertilization affects normal function. This study analyzes the potential from PRM1 as markers different breeds of bulls utilized artificial insemination programs, expected to be tool interpreting Indonesia. used Limousin, Holstein,...

The present study was conducted to investigate the effects of storage time and temperature porcine ovaries on quality nuclear maturation in vitro oocytes obtained from stored their subsequent development after fertilization. were physiological saline for 0, 3, 6, 9 12 h at various temperatures (4, 15, 25 35 C). pH follicular fluid ovaries, DNA fragmentation oocyte nucleus meiotic competence examined. Some C 6 fertilized vitro, then cultured 7 days examine ability embryos develop blastocyst...

This study aimed to analyze the abundance of protein protamine-2 in mature spermatozoa Ongole Grade bulls and its relation semen quality DNA fragmentation. A total 120 frozen straws from six aged four five years were used this study. The then divided into two groups, group (post-thawing motility >45%) B >45%). parameters included progressive motility, viability, plasma membrane integrity, sperm head defects, Progressive was analyzed using computer-assisted analysis. Sperm viability...

A variety of diluents, both homemade and commercial, are used in Indonesian artificial insemination centers to freeze bovine semen. One the diluents is Tris egg yolk. The yolk as a source lecithin protect sperm membrane during freezing. smaller particles, more effectively they can bind cell membrane. In addition, particle size facilitates analysis quality using Computer Assisted Sperm Analysis (CASA). This study aimed perform commercial analyzer (PSA). this were yolk, Andromed (Minitube,...

This article reviews research methods and analyses of bovine sperm DNA fragmentation conducted in Indonesia. A literature review using Google Scholar identified 37 articles by Indonesian authors published since 2018, with a peak eight 2023. The primary techniques employed include Acridine Orange staining (17 articles), Sperm-Bos-Halomax kits (12 Toluidine Blue (7 the TUNEL assay (1 article), each exhibiting methodological variations. provides valuable reference for stakeholders aiming to...

The present study was conducted to examine the effects of stage oestrous cycle on meiotic competence canine oocytes and also investigate relationship between relative size distribution obtained from bitches at three stages (anoestrus, follicular phase dioestrus). Only healthy-looking cumulus–oocyte complexes were used for in vitro maturation these divided into groups based diameter (<110, 110 <120 ≥120 µm). mean ovaries anoestrus, dioestrus 103.6, 119.2 107.7 µm, respectively....

The production of cloned animals is an inefficient process because early or late embryonic losses. This study focused on the DNA fragmentation that occurs during development. occurrence was examined in bovine embryos produced by vitro fertilization (IVF) and somatic cell nuclear transfer (NT) using terminal deoxynucleotidyl transferase (TdT) nick-end labelling (TUNEL). IVF NT at two-cell to blastocyst stage were stained TUNEL for analysis DNA-fragmented nuclei with propidium iodide...

Recent work has shown that glucose may induce cell injury through the action of free radicals generated by autooxidation or hypoxanthine phosphoribosyltransferase inhibition. The effect during early in vitro culture (IVC) period porcine embryos on their developmental competence, contents reactive oxygen species (ROS) and glutathione (GSH), quality blastocysts yielded was examined.In matured fertilized oocytes were cultured for first 2 days (Day 0 = day fertilization) IVC NCSU-37 added with...

We conducted two experiments to investigate the effects of storage temperature and period for cat ovaries on meiotic developmental competence oocytes collected from ovaries. In Experiment 1, were stored in physiological saline 24 h at 4 C, 23-25 or 38 C (cold, room, incubator groups, respectively), then each group. Morphologically intact selected cultured maturation medium h. Significantly more reached metaphase II (MII) cold group (53.4%) than room groups (20.0 2.4%, respectively). 2, 0, 6,...

Sericin is a water-soluble component of silk and has been used as biomaterial due to its antibacterial ultraviolet radiation-resistant properties. This study was designed evaluate the effect sericin supplementation in maturation medium on meiotic competence fertilisability sheep oocytes. Cumulus-oocyte complexes (COCs) were cultured TCM199 supplemented with at various concentrations 0 (control), 0.1, 0.25 0.5%, either or without bovine serum albumin (BSA). When COCs matured BSA, 0.1%...

POME contains a high concentration of chemi-cal oxygen demand (COD) ranging from 45,000-65,000 mg/L, biochemical (BOD) 21,500-28,5000 mg/L and total suspended solids (TSS) 15,660-23,560 (Wong et al., 2009; Chan 2013).Bello al. (2013) reported that oil grease content in being as 4,000 with pH between 3.4 4.7.High concentrations COD BOD harm aquatic organisms, for instance, decrease consumption cause hypoxic condition (Maygaonkar 2012; Verberk 2016; Vithana 2019).Oil are considered hazardous...

Abstract We investigated cAMP content, gap junctional communications (GJCs) status, and LH‐receptor (LH‐R) expression in porcine cumulus–oocyte complexes (COCs) during vitro maturation treated with the phosphodiesterase (PDE) inhibitor 3‐isobutyl‐1‐methylxanthine (IBMX) or FSH. COCs were cultured for 20 hr (1st culture) M199 containing 10% FBS (basic medium, BM group) supplemented FSH (FSH IBMX (IBMX group). Each COC was then transferred into both LH an additional 24 (2nd culture). The...

Background and Aim: Capacity for sperm production is affected by age, which related to the morphology of abnormalities can affect fertility. The aim this study was evaluate relationship between age concentrations testosterone adiponectin with in Simmental bulls. Materials Methods: used 11 bulls, separated into three groups. first group consisted five bulls aged 4-5 years, second third groups each 6-7 8-10 respectively. average motility animals ranged from 57.66±2.60% 70.17±0.22%. Blood...

Protamine (PRM) is the major protein in sperm nucleus and plays an essential role its normal function. Moreover, PRM has great potential as a marker of semen production quality. This study aimed to assess bovine quality bulls at National Artificial Insemination (AI) Center Indonesia. The capacity each bull was collected from frozen data Singosari AI for 6 months, then divided into two groups (high low). A total 440 straws six Limousin (LIM), Friesian Holstein (FH), Peranakan Ongole (PO),...

The effects of protein supplementation in culture medium on development to the hatching and hatched blastocyst stages cat vitro-fertilized embryos were investigated. In first experiment, presumptive zygotes derived from vitro maturation fertilization (IVF) cultured modified Earle's balanced salt solution (MK-1) supplemented with 0.4% bovine serum albumin (BSA) or 5% fetal (FBS) for 9 days. There no significant differences between BSA FBS groups respect proportion cleavage morula zygotes....

The successful of artificial insemination (AI) in buffalo is still low. quality frozen semen determines the AI program. Glycerol widely used for cryopreservation with unsatisfactory results due to its toxicity and antifertility properties. This study aimed investigate effect glycerol dimethylformamide (DMF) concentrations skim milk egg yolk (SMEY)–based diluent on frozen-thawed swamp bull semen. Fresh was divided into eight aliquots. Each aliquot diluted SMEY containing 4%, 5%, 6%, 7% DMF....

Objective: The present study analyzed the seminal plasma proteome and possible relationships between proteins semen quality in azoospermic normal Simmental bulls. Materials Methods: Fresh samples from Lembang Artificial Insemination Center were used for this study, including one bull (76´ ejaculate) with very poor quality/ azoospermia (poor fresh semen/infertile; PFS) three bulls (normal semen; NFS) proteomic analysis using a pooled system (NFS-Stud) (60´ ejaculate). only males obtained low...