A5053502532- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Renal and related cancers
- Genetic and phenotypic traits in livestock
- Tissue Engineering and Regenerative Medicine
- Reproductive Health and Technologies
- Seed Germination and Physiology
- Virus-based gene therapy research
- Veterinary Medicine and Surgery
- Xenotransplantation and immune response
- Bee Products Chemical Analysis
- Viral Infectious Diseases and Gene Expression in Insects
- Plant tissue culture and regeneration
- Mycotoxins in Agriculture and Food
- Effects of Environmental Stressors on Livestock
- Mesenchymal stem cell research
- Ruminant Nutrition and Digestive Physiology
- Selenium in Biological Systems
- Wheat and Barley Genetics and Pathology
- Animal health and immunology
Tokushima University
2016-2025
Guangdong Ocean University
2019-2022
Yamaguchi University
2008-2017
Institute for Animal Reproduction
2011-2015
Graduate School USA
2011
Texas A&M University
2002-2007
Kobe University
2004
Tohoku University
2004
Schön Klinik Neustadt
2004
University of Illinois Urbana-Champaign
2004
A new and highly efficient method for generating mutant pigs by electroporating the CRISPR/Cas9 system into zygotes.
The present study was conducted to investigate the effects of attachment cumulus cells porcine oocytes during process maturation and fertilization on nuclear maturation, subsequent development after in vitro (IVF). In first experiment, were removed from cumulus-oocyte complexes (COCs) at 0, 24 42 h onset culture then cultured until reaching cultivation. second COCs denuded as described fertilized for 7 days. As a control, allowed maintain end IVF. proportion metaphase II significantly...
Abstract Bovine blastocysts were produced through maturation, fertilization, and development in vitro. For vitrification, solutions designated EFS, GFS, PFS prepared; these 40% ethylene glycol, glycerol, propylene respectively, diluted modified phosphate‐buffered saline (PBS) containing 30% Ficoll + 0.5 M sucrose. The embryos exposed to the one step at room temperature, kept for various times, vitrified liquid nitrogen, warmed rapidly. When EFS solution after 1 or 2 min exposure, postwarming...
TP53 (which encodes p53) is one of the most frequently mutated genes in cancers. In this study, we generated TP53-mutant pigs by gene editing via electroporation Cas9 protein (GEEP), a process that involves introducing and single-guide RNA (sgRNA) targeting exon 3 intron 4 into vitro-fertilized zygotes. Zygotes modified sgRNAs were transferred to recipients, two which gave birth total 11 piglets. Of those piglets, 9 survived. Molecular genetic analysis confirmed 6 live piglets carried...
Contents Chlorogenic acid ( CGA ) is a quinic conjugate of caffeic acid, and phytochemical found in many fruits beverages that acts as an antioxidant. The present study investigated the effects supplementation during vitro maturation (IVM), on development porcine oocytes, to improve production IVP system. Oocytes were matured either without (control) or with (10, 50, 100 200 μM). Subsequently, oocytes fertilized cultured for 7 day. rates maturation, fertilization blastocyst formation 50 μM...
Cytoplasmic microinjection (CI) of the CRISPR/Cas9 system enabled induction site-specific mutations in porcine zygotes and resulting pigs. However, mosaicism is a serious problem for genetically modified In present study, we investigated suitable timing concentration components introduction into oocytes/zygotes by CI, to reduce blastocysts. First, introduced 20 ng/μl Cas9 protein guide RNA (gRNA), targeting α-1,3-galactosyltransferase (GalT) gene oocytes before vitro fertilization (IVF),...
CD163 is a putative fusion receptor for virus of porcine reproductive and respiratory syndrome (PRRS). In this study, we introduced CRISPR/Cas9 system [guide RNAs (gRNAs) with Cas9 protein] targeting the gene into in vitro-fertilized zygotes by electroporation to generate CD163-modified pigs. First, designed four types gRNAs that targeted distinct sites exon 7 gene. protein different was electroporation. When electroporated were allowed develop blastocysts vitro genome editing efficiency...
Abstract Background Xenoantigens are a major source of concern with regard to the success interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for biosynthesis galactosyl-alpha 1,3-galactose, xenoantigen causing hyperacute rejection. -modified pigs, therefore, promising donors pig-to-human xenotransplantation. In this study, we developed method introduction CRISPR/Cas9 system into in vitro-fertilized porcine zygotes via electroporation generate pigs. Results...
Abstract Objective Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into zygotes are used for generating genetically modified pigs. However, these methods create mosaic mutations in embryos. In this study, we evaluated whether gene editing method embryonic stage affect efficiency porcine Results First, designed five guide RNAs (gRNAs) targeting B4GALNT2 mutation by introducing each gRNA with Cas9 protein electroporation. Next, optimized was introduced 1-cell 2-cell...
The present study was conducted to investigate the effects of storage time and temperature porcine ovaries on quality nuclear maturation in vitro oocytes obtained from stored their subsequent development after fertilization. were physiological saline for 0, 3, 6, 9 12 h at various temperatures (4, 15, 25 35 C). pH follicular fluid ovaries, DNA fragmentation oocyte nucleus meiotic competence examined. Some C 6 fertilized vitro, then cultured 7 days examine ability embryos develop blastocyst...
Contents This study was conducted to determine suitable conditions for an experimental method in which the CRISPR /Cas9 system is introduced into vitro‐produced porcine zygotes by electroporation. In first experiment, when putative derived from vitro fertilization ( IVF ) were electroporated either unipolar or bipolar pulses, keeping voltage, pulse duration and number fixed at 30 V/mm, 1 msec five repeats, respectively, rate of blastocyst formation pulses decreased compared pulses. second...