A5041798041- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Electronic Packaging and Soldering Technologies
- Developmental Biology and Gene Regulation
- CRISPR and Genetic Engineering
- Congenital heart defects research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Intermetallics and Advanced Alloy Properties
- Wnt/β-catenin signaling in development and cancer
- Cancer Immunotherapy and Biomarkers
- Aluminum Alloy Microstructure Properties
- Genomics and Chromatin Dynamics
- 3D IC and TSV technologies
- Aluminum Alloys Composites Properties
- Immune Cell Function and Interaction
- Reproductive Biology and Fertility
- Renal and related cancers
- RNA Research and Splicing
- Advanced Welding Techniques Analysis
- CAR-T cell therapy research
- Sperm and Testicular Function
- Metallurgy and Material Science
- Fatigue and fracture mechanics
- Epigenetics and DNA Methylation
- Proteoglycans and glycosaminoglycans research
Tokushima University
2016-2025
Institute of Medical Sciences
2024
Tokushima College of Technology
2023
Osaka University
2006-2016
Shonan University of Medical Sciences
2014
Ube Frontier University
2009-2010
Harbin University of Science and Technology
2007
Shanghai Shipbuilding Technology Research Institute
1997-2006
Rutgers, The State University of New Jersey
1983
Targeted blockade of PD-1 with immune checkpoint inhibitors can activate T cells to destroy tumors. is believed function mainly at the effector, but not in activation, phase cell responses, yet how restricted activation stage currently unknown. Here we demonstrate that CD80 interacts PD-L1 cis on antigen-presenting (APCs) disrupt PD-L1/PD-1 binding. Subsequently, cannot engage inhibit when APCs express substantial amounts CD80. In knock-in mice which cis-PD-L1/CD80 interactions do occur,...
Abstract Recent use of the CRISPR/Cas9 system has dramatically reduced time required to produce mutant mice, but involvement a time-consuming microinjection step still hampers its application for high-throughput genetic analysis. Here we developed simple, highly efficient and large-scale genome editing method, in which RNAs are electroporated into zygotes rather than microinjected. We used this method perform single-stranded oligodeoxynucleotide (ssODN)-mediated knock-in mouse embryos. This...
A new and highly efficient method for generating mutant pigs by electroporating the CRISPR/Cas9 system into zygotes.
The expression of the transcription factor gene Sox2 precisely marks neural plate in various vertebrate species. We previously showed that prevailing chicken embryos is actually regulated by coordination five phylogenetically conserved enhancers having discrete regional coverage, among which 420-bp long enhancer N-1, active node-proximal region, probably involved directly genesis posterior plate. investigated signaling systems regulating this enhancer, first identifying 56-bp N-1 core...
TP53 (which encodes p53) is one of the most frequently mutated genes in cancers. In this study, we generated TP53-mutant pigs by gene editing via electroporation Cas9 protein (GEEP), a process that involves introducing and single-guide RNA (sgRNA) targeting exon 3 intron 4 into vitro-fertilized zygotes. Zygotes modified sgRNAs were transferred to recipients, two which gave birth total 11 piglets. Of those piglets, 9 survived. Molecular genetic analysis confirmed 6 live piglets carried...
Abstract The thymus is an organ that produces functionally competent T cells protect us from pathogens and malignancies. Foxn1 a transcription factor essential for organogenesis; however, the direct target to actuate thymic T-cell production unknown. Here we show Foxn1-binding cis-regulatory element promotes of β5t, which has role in cortical epithelial induce positive selection CD8 + cells. A point mutation this genome results defect β5t expression mice. reveal Foxn1-β5t transcriptional...
Contents This study was conducted to determine suitable conditions for an experimental method in which the CRISPR /Cas9 system is introduced into vitro‐produced porcine zygotes by electroporation. In first experiment, when putative derived from vitro fertilization ( IVF ) were electroporated either unipolar or bipolar pulses, keeping voltage, pulse duration and number fixed at 30 V/mm, 1 msec five repeats, respectively, rate of blastocyst formation pulses decreased compared pulses. second...
Regulation of gene expression requires selective incorporation histone H3 variant H3.3 into chromatin. Histone has several subsidiary variants but their functions are unclear. Here we characterize the function sub-variant, H3mm7, which is expressed in skeletal muscle satellite cells. H3mm7 knockout mice demonstrate an essential role regeneration. Chromatin analysis reveals that facilitates transcription by forming open chromatin structure around promoter regions including those myogenic...
ABSTRACT Chicken embryos are a powerful and widely used animal model in developmental biology studies. Since the development of CRISPR technology, gene-edited chickens have been generated by transferring primordial germ cells (PGCs) into recipients after genetic modifications. However, low inheritance caused competition between host transferred is common complication greatly reduces production efficiency. Here, we chicken, which can be ablated drug-dependent manner, as for PGC transfer. We...