A5018916327- Developmental Biology and Gene Regulation
- Connexins and lens biology
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Congenital heart defects research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Retinal Development and Disorders
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- Renal and related cancers
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Neurogenesis and neuroplasticity mechanisms
- Hedgehog Signaling Pathway Studies
- Biochemical effects in animals
- Virus-based gene therapy research
- Chromosomal and Genetic Variations
- Cancer-related gene regulation
- Signaling Pathways in Disease
- Molecular Biology Techniques and Applications
- Zebrafish Biomedical Research Applications
- Neuroblastoma Research and Treatments
Osaka University
2010-2024
Kyoto Sangyo University
2014-2024
Osaka Science Museum
2022-2024
Ube Frontier University
2004-2018
Frontier Science Foundation
2003-2017
Fracture Analysis Consultants (United States)
2017
Japan Science and Technology Agency
2001-2015
Bio-oriented Technology Research Advancement Institution
2008
University of Würzburg
2006
Kyoto Research Park
2005
Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling in vertebrates. The question remains unanswered, however, as to how these participate the Shh pathway. In this study, regulatory activities associated with Gli2 protein were investigated relation signaling. Although acts a weak transcriptional activator, it is fact composite positive and negative domains. cultured cells, truncation activation domain C-terminal half results repressor activity, while removal...
The floor plate plays important roles in ventral pattern formation and axonal guidance within the neural tube of vertebrate embryos. A critical event for development is induction a winged helix transcription factor, Hepatocyte Nuclear Factor-3beta (HNF-3beta). enhancer expression HNF-3beta located 3' unit consists multiple elements. depends on notochord-derived signal, Sonic hedgehog (Shh). Genetic analysis Drosophila has led to identification genes involved Hh signalling pathway, cubitus...
ABSTRACT Neuropilin is a type 1 membrane protein, which highly conserved among Xenopus frog, chicken and mouse. The extracellular part of the neuropilin protein composed three unique domains, each thought to be involved in molecular and/or cellular interactions. In mice, expressed cardiovascular system, nervous system limbs at particular developmental stages. To clarify roles morphogenesis vivo, we generated mouse embryonic stem (ES) cell clones that constitutively exogenous neuropilin, then...
Pax6 is a key transcription factor in eye development, particularly lens but its molecular action has not been clarified. We demonstrate that initiates development by forming complex with SOX2 on the lens-specific enhancer elements, e.g., δ- crystallin minimal DC5. DC5 shows limited similarity to binding consensus sequence of and bound poorly alone. However, binds cooperatively sequence, resulting formation high-mobility form ternary vitro, which correlates activation vivo. observed...
ZEB2 is a multi-zinc-finger transcription factor known to play significant role in early neurogenesis and epithelial-mesenchymal transition–dependent tumor metastasis. Although the function of T lymphocytes unknown, activity closely related family member ZEB1 has been implicated lymphocyte development. Here, we find that expression up-regulated by activated cells, specifically KLRG1hi effector CD8+ cell subset. Loss results loss antigen-specific cells after primary secondary infection with...
ABSTRACT Activation of the first lens-specific gene chicken, δ1-crystallin, is dependent on a group lens nuclear factors, δEF2, interacting with δ1-crystallin minimal enhancer, DC5. One δEF2 factors was previously identified as SOX2. We show that two related SOX proteins, SOX1 and SOX3, account for remaining members δEF2. DC5 enhancer their C-terminal domains. Expression Sox1-3 in eye region during induction studied comparison Pax6 δ?-crystallin. Pax6, known to be required inductive response...
ABSTRACT δEF1 is a DNA binding protein containing homeodomain and two zinc finger clusters, regarded as vertebrate homologue of zfh-1 (zinc homeodomain-containing factor-1) in Drosophila. In the developing embryo, expressed notochord, somites, limb, neural crest derivatives few restricted sites brain spinal cord. To elucidate regulatory function mouse embryogenesis, we generated null mutant (δEF1null(lacZ)) mice. The δEF1null(lacZ) homozygotes developed to term, but never survived...
Intermediate-filament Nestin and group B1 SOX transcription factors (SOX1/2/3) are often employed as markers for neural primordium, suggesting their regulatory link. We have identified adjacent essential POU factor binding sites in the enhancer. The 30-bp sequence of enhancer including these (Nes30) showed a nervous system-specific SOX-POU-dependent activity multimeric forms transfection assays was utilized assessing specificity synergism; combinations either or C (SOX11) with class III...
In the developing spinal cord, signals from roof plate are required for development of three classes dorsal interneuron: D1, D2, and D3, listed to ventral. Here, we demonstrate that absence Wnt1 Wnt3a, normally expressed in plate, leads diminished D1 D2 neurons a compensatory increase D3 neuron populations. This occurs without significantly altered expression BMP related genes plate. Moreover, Wnt3a protein induces markers isolated medial region chick neural Noggin does not interfere with...
We previously performed mutant screens in the medaka for defects gonadal development and identified a of interest this regard, which was designated as hotei (hot). This manifests number remarkable phenotypic abnormalities including: (i) excessive proliferation germ cells that initiates at around hatching stage regardless genetic sex fish; (ii) initiation premature meiosis phenotypically male hot homozygotes; (iii) one-half hot-homozygous XY fish undergo reversal, accompanies expression...
ABSTRACT We investigated nuclear factors that bind to δ1-crystallin enhancer core and regulate lens-specific transcription. A factor δEF1, which binds the essential element of core, was molecularly cloned from chicken by a southwestern method. The protein organization δEF1 deduced cDNA sequence indicated it has heterogeneous domains for DNA-binding, two widely separated zinc fingers homeodomain, analogous Drosophila ZFH-1 protein. C-terminal were found be responsible binding sequence. had...
The repressor delta EF1 was discovered by its action on the DC5 fragment of lens-specific 1-crystallin enhancer. C-proximal zinc fingers were found responsible for binding to and had specificity CACCT as revealed selection high-affinity sequences from a random oligonucleotide pool. is present not only in but also E2 box (CACCTG) elements which are sites various basic helix-loop-helix activators target an unidentified repressor, raising possibility that accounts activity. competed with E47...
Abstract The highest expression of the N-myc gene occurs during embryonic organogenesis in mouse ontogeny, with peak around day 9.5. Homozygous N-myc-deficient mice, produced by germline transmission a disrupted allele ES cells, developed normally to 10.5, indicating dispensability earlier period, but later accumulated organogenic abnormalities and died 11.5. most notable were found limb bud, visceral organs (lung, stomach, liver heart) central/peripheral nervous systems, highly correlated...
Recent findings suggest that Six3, a member of the evolutionarily conserved So/Six homeodomain family, plays an important role in vertebrate visual system development. However, little is known about molecular mechanisms by which this function accomplished. Although several members gene family interact with eyes absent (Eya) and as transcriptional activators, Six3 does not any Eya family. Here, we report Grg4 Grg5, mouse counterparts Drosophila co-repressor Groucho, its closely related Six6,...