We describe a reversed-phase HPLC method that uses gradient elution and diode array detection to quantitate eight biologically active phenolic constituents of wine: the cis trans isomers resveratrol their glucosides, catechin, epicatechin, quercetin, rutin. ODS Hypersil served as stationary phase; was formed by acetic acid, methanol, water. Each analysis required an equilibration period 10 min run time 40 for completion. Satisfactory peak resolution achieved following direct injection 20-μL...

To routinely assay the concentrations of ochratoxin A (OTA) in wines and beers, two new methods were developed evaluated. The first utilized solid-phase extraction on a C(18) cartridge to achieve 100-fold sample concentration followed by high-performance liquid chromatography column with gradient elution quantitation at 333 nm means photodiode array detector. Positive confirmation can be carried out purity match-factor analysis as well peak shift following esterification BF(3). Total run...

Determinations of serum alkaline phosphatase (APase) and 5′nucleotidase (5Nase) activities have been carried out in 159 children investigated for possible disease liver or bones, 147 whom these possibilities never arose. The latter group, which served as a control population, showed 5Nase levels very much lower than those encountered normal adult population. APase values this group rose after birth, fell the first year, remained stable until puberty when range increased slightly. was raised...

A simple immunofluorescence test for antibody to a mitochondrial antigen present in many tissues is reliable method of distinguishing most cases primary biliary cirrhosis from jaundice due extrahepatic tract obstruction. Of 30 diagnosed as cirrhosis, 26 had antimitochondrial whereas none 77 with bile duct obstruction showed this serological abnormality. The was also found the serum three 42 patients who other forms and two 266 no evidence liver disease. Clinical, biochemical, findings favour...

In recent years, online reviews have offered a rich new medium for consumers to express their opinions and feedback. Product designers frequently aim consider consumer preferences in work, but many firms are unsure of how best harness this feedback given that textual data is both unstructured voluminous. study, we use text mining tools propose method rapid prioritization reviews, differentiating the pertaining innovation opportunities most useful firms. We draw from entrepreneurship...

The concentrations of 11 phenols and 5 furans were measured in 12 categories distilled spirits by HPLC methodology, together with the total antioxidant status (TAS) same beverages. Ellagic acid was phenol present highest concentration all Moderate amounts syringaldehyde, syringic acid, gallic as well lesser vanillin vanillic measurable most samples whiskey, brandy, rum but largely undetectable gin, vodka, liqueurs, miscellaneous spirits. 5-(Hydroxymethyl)furfural predominant furan former...

Using a solid-phase extraction followed by direct-injection gas chromatography-mass spectrometry, the concentration of <i>trans</i>-resveratrol was measured in representative selection wines from most prominent wine-producing countries and regions world. With exception Swiss German wines, virtually all white tested had concentrations &lt;0.1 mg/L. Consistently high were Pinot noir, irrespective origin. On other hand, Cabernet Sauvignon covered wide range concentrations, with relatively...

The concentrations of 15 polyphenols (the phenolic acids gentisic, vanillic, ferulic, m-coumaric, p-coumaric, caffeic, and gallic acid; the trihydroxystilbenes cis- trans-resveratrol trans-polydatin; flavonoids, catechin, epicatechin, quercetin, morin) were measured in wines from a range white (Chardonnay, Riesling, Seyval Blanc, Vidal) red (Pinot Noir, Cabernet Sauvignon, Franc, Merlot, Gamay Noir) cultivars grown viticultural region Niagara, ON, Canada, to determine whether significant...

Eight serum enzyme tests were performed over a three-year period in 1,147 cases of patients with suspected hepatobiliary disease, whom 580 had identifiable primary disease the liver or biliary system. Individually, aminotransferase assays did not provide good discrimination among various categories but when expressed as ratio useful degree was obtained. Isocitrate dehydrogenase, guanase and glutamate dehydrogenase alone poor discriminants studied; combination latter aminotransferases ratios...

The enzyme Γ-glutamyl transpeptidase is widely distributed throughout the body, notably kidney, seminal vesicles, pancreas, liver, spleen and brain. Being one of enzymes cycle, it involved in aminoacid transport, catalysing a transpeptidation reaction between peptides most common amino acids. Methods assay are based on its ability also to act synthetic amides glutamic acid; kinetic methods monitoring release p-nitroaniline from substrate L-Γ-glutamyl p-nitroanilide satisfactory. In diseases...

Abstract Optimal concentrations of ammonium ions, 2-oxoglutarate, and NADH were defined for serum glutamate dehydrogenase (EC 1.4.1.2) activity at 37°C. Substrate inhibition was not observed with the last two compounds. The pH optimum in triethanolamine buffer is 7.4, inhibited by increasing concentration beyond 50 mmol/liter. Activation ADP exceeds that promoted L-leucine, there little advantage having both present. Activity linear time enzyme to a limiting absorbance change 0.300 340 nm,...