This study examined the relationship between feed efficiency and performance, feeding behavior, blood metabolic variables, various ultrasonic measurements in finishing beef heifers. Within-animal repeatability estimates of intake efficiency, body measures, plasma analytes across growing stages lifespan animal were also calculated. Fifty heifers previously ranked as yearlings on phenotypic residual (RFI) used. Animals [initial BW = 418 (SD 31.5) kg] offered a TMR diet consisting 70:30...

Abstract BACKGROUND: In experiment 1, 30 boars were assigned to one of five treatments ( n = 6): T1, 0 g kg −1 seaweed extract (SWE); T2, 0.7 SWE; T3, 1.4 T4, 2.8 SWE and T5, 5.6 SWE. The contained laminarin fucoidan only was extracted from Laminaria spp. 2, 28 assigned, in a 2 × factorial four 7): control; control plus 300 mg laminarin; 240 fucoidan; diet. RESULTS: 1 there response on colonic Bifidobacterium P < 0.01 quadratic), Enterobacterium (quadratic 0.05) caecal 0.05). an...

A 2 × factorial experiment was conducted to investigate the interactions between laminarin (LAM; 0 and 300 parts per million (ppm)) fucoidan (FUC; 240 ppm) levels on intestinal morphology, selected microbiota inflammatory cytokine gene expression in weaned pig. There an interaction LAM FUC supplementation Enterobacteriaceae population ( P < 0·05) abundance of attaching effacing Escherichia coli (AEEC) strains colon. Pigs offered diet had a reduced compared with pigs basal diet. However,...

The objective of this study was to determine the effect hydrothermal-assisted extraction (HAE) and a multi-step purification process on molecular characteristics antioxidant activity extracts laminarin from brown seaweed Laminaria hyperborea. HAE with optimised conditions (time - 30 min; temperature 99.3 °C; sample solvent ratio 1:21.3 (w/v)) resulted in 368 mg/g Crude Extract. Purification using solvents, weight cut-off filter (MWCO; 10 kDa) solid-phase (SPE) improved concentration 542.6...

The aim of this study was to determine the anti-inflammatory potential pomegranate peel extracts (PPE) prepared from waste material juice production both in vitro on Caco-2 cells and ex vivo using porcine colonic tissue explants. were stimulated by TNF explants with lipopolysaccharide (LPS). Both tissues co-treated PPE at 0, 1.0, 2.5, 5.0, 10 25 μg/mL. secretion CXCL8 supernatant experiments determined enzyme linked immunosorbent assay (ELISA) relative expression inflammatory cytokines...

The main objective was to determine the chemical, phytochemical, fatty acid and mineral profiles of three commercially relevant brown macroalgae (Laminaria digitata, Laminaria hyperborea Ascophyllum nodosum) collected each season for two years off west coast Ireland. All minerals analysed varied significantly depending on macroalgal species, year collection. Overall, protein contents were negatively correlated with carbohydrate content. Protein (2–11%) at its highest during winter and/or...

An experiment (complete randomised design) was conducted to investigate the effects of Laminaria hyperborea and digitata seaweed extract inclusion on gut morphology, selected intestinal microbiota populations, volatile fatty acid concentrations immune status weaned pig. Twenty-eight piglets (24 days age, 6.5 ± 1.4 kg live weight) were assigned one four dietary treatments for 7 then sacrificed: (T1) basal diet (control); (T2) 1.5 g/kg L. extract; (T3) (T4) a combination extract. The contained...

ABSTRACT A procedure for discrimination between scrapie and bovine spongiform encephalopathy (BSE) in sheep is of importance establishing whether BSE has entered the population. Since not yet been found at farm level, such procedures can be developed only with experimental BSE. Two distinctive molecular features prion protein (PrP)—molecular size glycosylation profile—in proteinase K digests brain stem tissue from were used here; upon Western blotting, these led to an unequivocal among...

An experiment with a 2 × factorial arrangement of treatments (n = 10 sows/treatment) was conducted to investigate the effect maternal dietary supplementation seaweed extract (SWE: 0 vs. 10.0 g/d) and fish oil (FO) inclusion (0 100 from d 109 gestation until weaning (d 26) on sow colostrum milk composition, humoral immune response 5 12 lactation, suckling piglet performance. Furthermore, influence treatment phagocytic activity whole blood white cells at examined. The SWE (10 g) contained...

β-Glucans have been identified as natural biomolecules with immunomodulatory activity. The first objective of the present study was to compare effects purified β-glucans derived from Laminaria digitata , L. hyperborea and Saccharomyces cerevisiae on piglet performance, selected bacterial populations intestinal volatile fatty acid (VFA) production. second aim gene expression profiles markers pro- anti-inflammation in both unchallenged lipopolysaccharide (LPS)-challenged ileal colonic tissues....

ABSTRACT Non-O157 Shiga toxin-producing Escherichia coli (STEC) strains are clinically significant food-borne pathogens. However, there is a dearth of information on serotype prevalence and virulence gene distribution, data essential for the development public health protection monitoring control activities meat dairy industries. Thus, objective this study was to examine non-O157 STEC beef farms characterize isolates in terms markers. Bovine fecal samples ( n = 1,200) farm soil 600) were...

Chitosan, a natural polysaccharide comprising copolymers of glucosamine and N-acetylglucosamine, has been shown to have anti-obesity properties. Two experiments (Exp. 1 Exp. 2) were performed determine the role chitosan on dietary intake, body weight gain, fat deposition in pig model, as well identifying potential mechanisms underlying effect chitosan. In 1, nutrient digestibility experiment, 16 pigs (n = 4/treatment) randomly allocated one four treatments follows: 1) basal diet; diet plus...

The aim of the present study was to establish optimum inclusion level laminarin derived from Laminaria digitata on selected microbial populations, intestinal fermentation, cytokine and mucin gene expression in porcine ileum colon. A total twenty-one pigs (mean body weight 17·9 kg) were randomly assigned one three dietary treatments: T1 – basal (control) diet, T2 T3 diets supplemented with included at 300 600 parts per million (ppm), respectively. Selected bacterial populations volatile fatty...

The objectives of this study were to employ response surface methodology (RSM) investigate and optimize the effect ultrasound-assisted extraction (UAE) variables, temperature, time amplitude on yields polysaccharides (fucose total glucans) antioxidant activities (ferric reducing power (FRAP) 1,1-diphenyl-2-picryl-hydrazyl radical scavenging activity (DPPH)) from Laminaria digitata, explore suitability applying optimum UAE conditions for L. digitata other brown macroalgae (L. hyperborea...

Fucose sulphated polysaccharides (FSPs) and glucans have recently attracted the attention of scientific community due to their wide range biological activities. Both should ideally be selectively extracted using innovative technologies with high extraction efficiency. This study aims to: (1) Optimise variables used in hydrothermal-assisted (HAE) obtain yields FSPs, total glucans, antioxidants from

In the present study, two experiments were conducted to (1) evaluate effect of laminarin and/or fucoidan on ileal morphology, nutrient transporter gene expression and coefficient total tract apparent digestibility (CTTAD) nutrients (2) determine whether inclusion could be used as an alternative ZnO supplementation in weaned pig diets. Expt 1 was designed a 2 × factorial arrangement, comprising four dietary treatments ( n 7 replicates, weaning age 24 d, live weight 6·9 kg). The follows: basal...

The present study investigates the molecular characteristics of fucoidan obtained from brown Irish seaweed

Abstract Twenty piglets (21 days, 7·8 kg live weight (LW)) were used in a 2×2 factorial to investigate interactions between lactose and inulin on intestinal morphology, microbiology volatile fatty acid (VFA) production of the weanling pig. The offered following diets for 6 days then sacrificed: (T1) 150 g/kg lactose, (T2) +15 inulin, ( T3) 330 T4) inulin. Tissue samples taken from duodenum, jejunum ileum morphological measurements. Digesta ileum, caecum colon. There was an interaction...

The objective of this study was to determine the effect carprofen (C) administration before banding or burdizzo castration bulls on cortisol, in vitro interferon-γ (IFN-γ) production, acute-phase proteins, feed intake, and growth. Fifty Holstein Friesian (5.5 mo old; 191 ± 3.7 kg) were blocked by weight assigned randomly 1 5 treatments (n = 10/treatment): 1) untreated control (2) at 0 min (Band); 3) Band following an i.v. injection 1.4 mg/kg BW C −20 (Band+C); 4) Burdizzo (Burd); 5) Burd...