A5037653832- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Molecular Junctions and Nanostructures
- RNA and protein synthesis mechanisms
- Virus-based gene therapy research
- Advanced Biosensing Techniques and Applications
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Nanopore and Nanochannel Transport Studies
- Child Development and Digital Technology
- Muscle Physiology and Disorders
- Analytical Chemistry and Chromatography
- Innovative Teaching Methods
- Advanced Fluorescence Microscopy Techniques
- Single-cell and spatial transcriptomics
- Heart Failure Treatment and Management
- Cardiomyopathy and Myosin Studies
- Mobile Learning in Education
- Resilience and Mental Health
- Viral Infectious Diseases and Gene Expression in Insects
- Cardiovascular Effects of Exercise
University of Minnesota System
2019-2024
University of Minnesota
2017-2023
University of Minnesota Medical Center
2019
Efficient cell-free protein expression from linear DNA templates has remained a challenge primarily due to template degradation. In addition, the yields of transcription in systems lag behind transcriptional efficiency live cells. Most commonly used vitro translation utilize T7 RNA polymerase, which is also enzyme included many commercial kits.Here we present characterization variant polymerase promoter that acts significantly increase gene within systems. We have demonstrated T7Max...
Isothermal, cell-free, synthetic biology-based approaches to pathogen detection leverage the power of tools available in biological systems, such as highly active polymerases compatible with lyophilization, without complexity inherent live-cell which nucleic acid sequence based amplification (NASBA) is well known. Despite reduced associated cell-free side reactions are a common characteristic these systems. As result, systems often exhibit false positives from lacking an amplicon. Here we...
Abstract Structural biology education commonly employs molecular visualization software, such as PyMol, RasMol, and VMD, to allow students appreciate structure–function relationships in biomolecules. In on‐ground, classroom‐based education, these programs are used on University‐owned devices with software preinstalled. Remote typically involves the use of student‐owned devices, which complicates owing fact that (a) student have differing configurations (e.g., Windows vs MacOS) processing...
A teaching laboratory experiment is described where students prepare in vitro transcription reactions of a fluorescent RNA aptamer, named Broccoli, and observe the production aptamer real-time on fluorescence plate reader. Alternate visualization methods with minimal costs are also for laboratories lacking this instrumentation. Two optional experiments described. Optional Experiment 1 involves purification using commercial spin column kit having correlate cleanup yield transcribed...
Abstract Biological computation is becoming a viable and fast-growing alternative to traditional electronic computing. Here we present biocomputing technology called Trumpet: Transcriptional RNA Universal Multi-Purpose GatE PlaTform. Trumpet combines the simplicity robustness of simplest in vitro methods, adding signal amplification programmability, while avoiding common shortcomings live cell-based solutions. We have demonstrated use build all universal Boolean logic gates. also built...
Abstract Here we demonstrate a switchable DNA electron‐transfer catalyst, enabled by selective destabilization of secondary structure the denaturant, perchlorate. The system is comprised two strands, one which can be selectively switched between G‐quadruplex and duplex or single‐stranded conformations. In state, it binds hemin, enabling peroxidase activity. This switching ability arises from our finding that perchlorate, chaotropic Hofmeister ion, destabilizes over DNA. By varying...
Abstract Efficient cell-free protein expression from linear DNA templates has remained a challenge primarily due to template degradation. Here we present modified T7 RNA polymerase promoter that acts significantly increase the yields of both transcription and translation within in vitro systems. The promoter, termed T7Max, recruits standard polymerase, so no engineering is needed take advantage this method. This technique could be used with any polymerase-based system. Unlike other methods...
The SARS-CoV-2 pandemic underscored the need for early, rapid, and widespread pathogen detection tests that are readily accessible. Many existing rapid isothermal methods employ recombinase polymerase amplification (RPA), which exhibits PCR-like sensitivity, specificity, even higher speed. However, coupling RPA to other enzymatic reactions has proven difficult. For first time, we demonstrate with tuning of buffer conditions optimization reagent concentrations, can be cascaded into an in...
Abstract Here we demonstrate that a DNA nanodevice can perform switchable electron transfer. The is comprised of two strands, one which be selectively switched between G-quadruplex and duplex or single-stranded conformations. In the state, it binds cofactor hemin, enabling peroxidase activity. This switching ability arises from our discovery perchlorate, chaotropic Hofmeister ion, destabilizes over DNA. By varying perchlorate concentration, show device states do not catalyze transfer...
Abstract Isothermal, cell-free, synthetic biology-based approaches to pathogen detection leverage the power of tools available in biological systems, such as highly active polymerases compatible with lyophilization, without complexity inherent live-cell which Nucleic Acid Sequence Based Amplification (NASBA) is well known. Despite reduced associated cell-free side reactions are a common characteristic these systems. As result, systems often exhibit false positives from lacking an amplicon....