A5046391729- Plant Physiology and Cultivation Studies
- Postharvest Quality and Shelf Life Management
- Horticultural and Viticultural Research
- Plant Reproductive Biology
- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Banana Cultivation and Research
- Plant Pathogens and Fungal Diseases
- Flowering Plant Growth and Cultivation
- Erythrocyte Function and Pathophysiology
- Plant and animal studies
- Endoplasmic Reticulum Stress and Disease
- Cosmology and Gravitation Theories
- Microtubule and mitosis dynamics
- Signaling Pathways in Disease
- Cell Image Analysis Techniques
- Polyamine Metabolism and Applications
- Autophagy in Disease and Therapy
- Geophysics and Gravity Measurements
- Plant Molecular Biology Research
- Plant responses to water stress
- Plant biochemistry and biosynthesis
- Garlic and Onion Studies
- Plant Stress Responses and Tolerance
- Calcium signaling and nucleotide metabolism
Tokyo Women's Medical University
2014-2024
Toyota Technological Institute
2021-2024
Tottori University
2003-2020
Okayama University
1964-2018
RIKEN Nishina Center
2018
Toray (United States)
2009-2014
Meguro Parasitological Museum
2013
Toray Industries, Inc. (Japan)
2013
Tottori Prefectural Museum
2011
Institute of Vegetable and Floriculture Science
2011
Possible roles of anti-nephrin antibodies in post-transplant recurrent focal segmental glomerulosclerosis (FSGS) have been reported recently. To confirm these preliminary results, we performed a multi-institutional study 22 Japanese pediatric kidney transplant recipients with FSGS including eight genetic and 14 non-genetic (presumed primary) FSGS. Eleven the patients had Median (interquartile range) plasma levels measured using ELISA were markedly high at 899 (831, 1292) U/mL (cutoff 231...
Dynamin is a fission protein that participates in endocytic vesicle formation. Although dynamin was originally identified as microtubule-binding protein, the physiological relevance of this function unclear. Recently, mutations ubiquitously expressed 2 (dyn2) were found patients with Charcot-Marie-Tooth (CMT) disease, which an inherited peripheral neuropathy. In study, we show one these mutations, 551Δ3, induces prominent decoration microtubules mutant dyn2. Dyn2 required for proper dynamic...
The early endosome acts as a sorting station for internalized molecules destined recycling or degradation. While recycled are sorted and delivered to tubular endosomes, residual compartments containing be degraded undergo "maturation" before final degradation in the lysosome. This maturation involves acidification, microtubule-dependent motility, perinuclear localization. It is currently unknown how processes of cooperate with each other. Here, we show that fission triggers endosome, leading...
• Background and Aims Dramatic increases in fruit size have accompanied the domestication of Pyrus pyrifolia. To evaluate contribution cell division enlargement evolution size, following study was conducted. Methods Three wild 46 cultivated pyrifolia cultivars were selected to examine number/size at time pollination harvest. The period estimated by logarithmic curve increasing pattern number, its correlations with maturation final analysed. Key Results Final is directly related number cells...
A total of 118 Pyrus sp. (pear) and cultivars native mainly to east Asia were subjected randomly amplified polymorphic DNA (RAPD) analysis evaluate genetic variation relationships among the accessions. Two hundred fifty RAPD markers scored from 20 decamer primers. specific species identified. Clustering revealed two divisions: one comprising P. communis L., other including all accessions Asia. The grouping by data largely agrees with morphological pear taxonomy. However, some noted...
ADP-ribosylation factor 6 (Arf6) is a small-GTPase that regulates the membrane trafficking between plasma and endosome. It also involved in reorganization of actin cytoskeleton. GTPase-activating protein (GAP) critical regulator Arf function as it inactivates Arf. Here, we identified novel species GAP denoted SMAP1 preferentially acts on Arf6. Although overexpression did not alter subcellular distribution cytoskeleton, block endocytosis transferrin receptors. Knock down endogenous abolished...
We recently reported that SMAP1, a GTPase-activating protein (GAP) for Arf6, directly interacts with clathrin and regulates the clathrin-dependent endocytosis of transferrin receptors from plasma membrane. Here, we identified SMAP1 homologue named SMAP2. Like SMAP2 exhibits GAP activity heavy chain (CHC). Furthermore, show assembly CALM. Unlike however, appears to be regulator Arf1 in vivo, because cells transfected GAP-negative mutant were resistant brefeldin A. colocalized adaptor proteins...
To clarify the relationship between pollen density and gametophytic competition in Pyrus pyrifolia, performance, gibberellin metabolism, fruit set, quality were investigated by modifying P. pyrifolia grain number with Lycopodium spores. Higher levels of improved seed viability, quality. Treatments highest showed a significantly increased growth rate larger at harvest. High germination gave faster tube growth, both vivo vitro. Endogenous (GA) concentrations tubes soon after concentration two...
Phosphatidylinositol binding clathrin assembly protein (PICALM), also known as lymphoid myeloid leukemia (CALM), was originally isolated part of the fusion gene CALM/AF10, which results from chromosomal translocation t(10;11)(p13;q14). CALM is sufficient to drive in vitro on lipid monolayers and regulates clathrin-coated budding size shape vesicles at plasma membrane. However, physiological role has yet be elucidated. Here, vivo investigated using CALM-deficient mice. mice exhibited retarded...
Alloreactive memory T cells are present in virtually all transplant recipients due to prior sensitization or heterologous immunity and mediate injury undermining graft outcome. In mouse models, endogenous CD8 infiltrate MHC-mismatched cardiac allografts produce IFN-γ response donor class I MHC within 24 h posttransplant. The current studies analyzed the efficacy of anti-LFA-1 mAb inhibit early cell allograft infiltration activation. Anti-LFA-1 given C57BL/6 6 (H-2(b)) A/J (H-2(a)) heart...
Phosphatidylinositol 4-kinase IIα (PtdIns4KIIα) localizes to the trans-Golgi network and endosomal compartments has been implicated in regulation of traffic, but roles both its enzymatic activity site action have not elucidated. This study shows that PtdIns4KIIα is required for production phosphatidylinositol 4-phosphate (PtdIns(4)P) on early endosomes sorting transferrin epidermal growth factor receptor into recycling degradative pathways. Depletion with small interfering RNA significantly...
In Japanese pear, the application of GA3+4 during period rapid fruit growth resulted in a marked increase pedicel diameter and bigger at harvest. To elucidate relationship between capacity to determine main factor responsible for larger size harvest, vascularization after GA were examined carbohydrate fluxes monitored spur unit by non-invasive techniques using 13C tracer. Histological studies revealed that increased cell mesocarp but not number core size. The investigation carbon...
The trans-Golgi network (TGN) functions as a hub organelle in the exocytosis of clathrin-coated membrane vesicles, and SMAP2 is an Arf GTPase-activating protein that binds to both clathrin assembly (CALM). In present study, detected on TGN pachytene spermatocyte round spermatid stages spermatogenesis. Gene targeting reveals SMAP2-deficient male mice are healthy survive adulthood but infertile exhibit globozoospermia. spermatids, diameter proacrosomal vesicles budding from increases,...
A Heat Flux Sensor (HFS) facilitates the visualization of heat flow, unlike a temperature sensor, and is anticipated to be key technology in managing waste heat. Recently, an HFS utilizing Anomalous Nernst Effect (ANE) has been proposed garnering significant interest enhancing transverse thermopower. However, ideal materials for not only require large thermopower but also meet several criteria including low thermal conductivity bipolar nature thermopower, especially negative In this study,...
SummaryThirteen native pear species have been identified in China, of which P. armeniacaefolia Yu and sinkiangensis are specific to Xinjiang. grows wild a few cultivars assigned this species. Cultivars suspected be hybrid origin involving communis L. bretschneideri Rehd. In study, traditional Xinjiang were evaluated using RAPD markers compared with representatives Occidental species, East Asian accessions. The combination 72 accessions 20 selected primers produced 231 scorable polymorphic...
The heterodimeric transcription factor PEBP2/CBF is composed of a DNA-binding subunit, called Runx1, and non-DNA-binding PEBP2beta/CBFbeta. Runx1 protein detected exclusively in the nuclei most cells tissues, whereas PEBP2beta located cytoplasm. We addressed mechanism by which localizes to cytoplasm found that it associated with filamin A, an actin-binding protein. Filamin A retains cytoplasm, thereby hindering its engagement as partner. interaction mediated region within includes amino acid...
Both ‘Atago’ and ‘Shinkou’ are late-season japanese pear ( Pyrus pyrifolia Nakai) cultivars with russet-brown fruit, the progenies of crosses made between ‘Nijisseiki’ unknown cultivars, but they display different growth habit, fruit size, quality. To clarify difference in development two levels endogenous gibberellins (GA 1 , GA 3 4 ) abscisic acid (ABA) were identified quantified by gas chromatography/mass spectrometry, a histological study was carried out. The results showed that cell...
Early endosomes (EEs) are known to be a sorting station for internalized molecules destined degradation, recycling, or other intracellular organelles. Segregation is an essential step in such sorting, but the molecular mechanism of this process remains elucidated. Here, we show that actin required efficient recycling and endosomal maturation by producing motile force. Perturbation dynamics drugs induced few enlarged EEs containing several degradative vacuoles also interfered with their...
The formation of clathrin-coated vesicles is essential for intracellular membrane trafficking between subcellular compartments and triggered by the ARF family small GTPases. We previously identified SMAP1 as an ARF6 GTPase-activating protein that functions in clathrin-dependent endocytosis. Because abnormalities are often associated with oncogenesis, we targeted Smap1 mice to examine its physiological pathological significance. Smap1-deficent exhibited healthy growth, but their erythroblasts...