A5017659795- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Diabetes and associated disorders
- Pancreatic function and diabetes
- Peptidase Inhibition and Analysis
- Adipose Tissue and Metabolism
- Growth Hormone and Insulin-like Growth Factors
- Adipokines, Inflammation, and Metabolic Diseases
- Metabolism, Diabetes, and Cancer
- Enzyme Catalysis and Immobilization
- Calcium signaling and nucleotide metabolism
- Biochemical and Molecular Research
- Respiratory viral infections research
Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry
2020-2024
University of Chemistry and Technology, Prague
2021
European Molecular Biology Laboratory
2019
Toxin-antitoxin (TA) systems regulate fundamental cellular processes in bacteria and represent potential therapeutic targets. We report a new RES-Xre TA system multiple human pathogens, including Mycobacterium tuberculosis. The toxin, MbcT, is bactericidal unless neutralized by its antitoxin MbcA. To investigate the mechanism, we solved 1.8 Å-resolution crystal structure of MbcTA complex. found that MbcT resembles secreted NAD+-dependent bacterial exotoxins, such as diphtheria toxin. Indeed,...
The insulin/IGF superfamily is conserved across vertebrates and invertebrates. Our team has identified five viruses containing genes encoding viral insulin/IGF-1 like peptides (VILPs) closely resembling human insulin IGF-1. This study aims to characterize the impact of Mandarin fish ranavirus (MFRV) Lymphocystis disease virus-Sa (LCDV-Sa) VILPs on system for first time. We chemically synthesized single chain (sc, IGF-1 like) double (dc, forms MFRV LCDV-Sa VILPs. Using cell lines...
Members of the insulin/insulin-like growth factor (IGF) superfamily are well conserved across evolutionary tree. We recently showed that four viruses in Iridoviridae family possess genes encode proteins highly homologous to human insulin/IGF-1. Using chemically synthesized single-chain (sc), i.e., IGF-1-like, forms viral insulin/IGF-1-like peptides (VILPs), we previously they can stimulate receptors. Because these potential cleavage sites form double chain (dc), more insulin-like, VILPs,...
Insulin is a lifesaver for millions of diabetic patients. There need new insulin analogues with more physiological profiles and that will be thermally stable than human insulin. Here, we describe the chemical engineering 48 were designed to have changed binding specificities toward isoforms A B receptor (IR-A IR-B). We systematically modified at C-terminus B-chain, N-terminus A-chain, A14 A18 positions. discovered an analogue has Cα-carboxyamidated Glu B31 Ala B29 3-fold-enhanced specificity...
ABSTRACT Members of the insulin/IGF superfamily are well conserved across evolutionary tree. We recently showed that four viruses in Iridoviridae family possess genes encode proteins highly homologous to human insulin/IGF-1. Using chemically synthesized single chain (sc), i.e. IGF-1-like, forms viral insulin/IGF-1 like peptides (VILPs), we previously they can stimulate receptors. Because these potential cleavage sites form double (dc), more insulin-like, VILPs, this study, have characterized...
Abstract The members of the insulin superfamily are well conserved across evolution tree. We recently showed that four viruses in Iridoviridae family possess genes share high similarity with human and IGF-1. By chemically synthesizing single chain (sc, IGF-1 like) forms these viral insulin/IGF-1 like peptides (VILPs), we previously sc VILPs have insulin/IGF properties vitro vivo. However, characteristics double (dc, insulin-like) remain unknown. In this study, characterized dc for Grouper...