A5101811600- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Corneal Surgery and Treatments
- Ophthalmology and Visual Impairment Studies
- Metal-Catalyzed Oxygenation Mechanisms
- Cancer Research and Treatments
- Marine Sponges and Natural Products
- Steroid Chemistry and Biochemistry
- Drug Transport and Resistance Mechanisms
- Carbon dioxide utilization in catalysis
- Chromatography in Natural Products
- Porphyrin Metabolism and Disorders
- Microbial Natural Products and Biosynthesis
- Natural product bioactivities and synthesis
- Biochemical and biochemical processes
- Catalysis for Biomass Conversion
- Amino Acid Enzymes and Metabolism
- Metal complexes synthesis and properties
- Enzyme Structure and Function
- Phytochemistry and Bioactivity Studies
- Cerebral Venous Sinus Thrombosis
- Enzyme Catalysis and Immobilization
- Porphyrin and Phthalocyanine Chemistry
- Microbial Metabolism and Applications
- Ocular Diseases and Behçet’s Syndrome
Shandong University
2017-2024
Shandong Eye Hospital
2022-2024
Shandong First Medical University
2021-2024
Dalian Medical University
2009-2010
Ergothioneine synthase (EgtB) is a unique non-heme mononuclear iron enzyme that catalyzes the sulfoxidation and C–S bond formation between γ-glutamyl cysteine (γGC) N-α-trimethyl histidine (TMH) as pivotal step in ergothioneine biosynthesis. A controversy has arisen regarding sequence of catalytic cycle. To clarify this issue, QM/MM approach been employed to investigate detailed mechanism EgtB. Two binding modes O2 Fe(II) ("end-on" "side-on") have identified. Within present computational...
Ubiquinone plays a pivotal role in the aerobic cellular respiratory electron transport chain, whereas ferulic acid decarboxylase (FDC) is involved biosynthesis of ubiquinone precursor. Recently, complete crystal structure FDC (based on co-expression A. niger fdc1 gene E. coli with associated ubix from coli) at high resolution was reported. Herein, detailed catalytic non-oxidative decarboxylation mechanism has been investigated by combined quantum mechanics/molecular mechanics (QM/MM)...
Cinobufagin (1) is a major bufadienolide in ChanSu (a traditional Chinese medicine) with wide range of pharmacological activities. In this paper, the vivo metabolites 1 rats were studied.Nine isolated from bile rats, and their structures identified as: desacetylcinobufagin (2), 3-ketodesacetylcinobufagin (3), 3-epi-desacetylcinobufagin (4); 5β-hydroxy-3-epi-desacetylcinobufagin (5), 1α-hydroxy-3-epi-desacetylcinobufagin (6), 12β-hydroxy-3-epi-desacetylcinobufagin (7),...
To evaluate regional changes in the posterior corneal elevation after three laser refractive surgeries for correction of myopia different degrees.
The catalytic mechanism of Ni-QueD<sup>FLA</sup> was elucidated by QM/MM calculations, and the different reactivities nickel iron were illuminated.
To observe and analyze the clinical multi-mode imaging features of eyes with PHOMS, to introduce two cases PHOMS which underwent imaging.Retrospective observational study. A total 26 patients (37 eyes) hyperreflective structures surrounded by hyporeflective edges around optic discs who were examined diagnosed at Shandong Eye Hospital between January 2019 June 2021 included in Among these patients, 12 male 14 female. Fifteen monocular. The average age was 39 years. All following examinations:...
Metabolic blockade-based genome mining of the marine sediment-derived
The peroxide-dependent coproheme decarboxylase ChdC from Geobacillus stearothermophilus catalyzes two key steps in the synthesis of heme b, i.e., sequential oxidative decarboxylations coproporphyrinogen III (coproheme III) at propionate groups P2 and P4. In binding site III, P4 are anchored by different residues (Tyr144, Arg217, Ser222 for Tyr113, Lys148, Trp156 P4); however, strong experimental evidence supports that generated Tyr144 radical acts as an unique intermediary hydrogen atom...
The aim of this study was to compare corneal biomechanics treated with femtosecond laser-assisted in situ keratomileusis (FS-LASIK) and small-incision lenticule extraction (SMILE) for myopia astigmatism using the new biomechanical parameters Corvis ST II.This a prospective nonrandomized controlled study. Patients FS-LASIK or SMILE between January 2018 July were included. II performed measure parameters, including deformation amplitude ratio 2.0 mm (DA mm), integrated inverse radius (Integr...
To compare the 6-month changes in aberration and biomechanics after femtosecond laser-assisted laser situ keratomileusis (FS-LASIK) for high astigmatism.In this retrospective case control study, 47 eyes with astigmatism (≥ 2.5 D, HA group) low (≤ 1.0 LA underwent FS-LASIK. Preoperative follow-up examinations included visual outcomes, higher order aberrations (HOAs) biomechanics. Biomechanical parameters include a deformation amplitude ratio of 2 mm (DA mm), integrated inverse radius (IIR),...
<title>Abstract</title> Background To compare the corneal biomechanical parameters and higher-order aberrations (HOAs) of thin normal cornea (TNC) Forme Fruste keratoconus (FFKC). Methods The data collected between June 2018 May 2023 at Eye Hospital Affiliated with Shandong First Medical University were divided into two groups: 26 eyes patients (18–38 years) thinnest thickness (TCT) ≤ 500 µm (14–35 FFKC. Central (CCT) biomechanical-corrected intraocular pressure (bIOP) matched by propensity...
Objective: To evaluate the initial safety and efficacy of femtosecond laser-assisted minimally invasive lamellar keratoplasty (FL-MILK) for advanced keratoconus. Methods: It was a case series study. Patients with keratoconus who underwent FL-MILK between August 2017 April 2020 at Shandong Eye Hospital were prospectively included. The laser used to create an intrastromal pocket in recipient cornea donor. then gently inserted into through incision flattened. Clinical measurements included...
<title>Abstract</title> Objectives To evaluate the regional changes of posterior corneal elevation after three laser refractive surgeries for correction myopia different degrees. Methods 200 patients (200 eyes) who underwent epithelial keratoplasty (LASEK), femtosecond-assisted in situ keratomileusis (FS-LASIK) and small-incision lenticule extraction (SMILE) were included this retrospective, comparative non-randomized study. According to preoperative spherical equivalent (SE), each surgical...