A5064047240- Corneal Surgery and Treatments
- Corneal surgery and disorders
- Ocular Surface and Contact Lens
- Ocular Infections and Treatments
- Intraocular Surgery and Lenses
- Glaucoma and retinal disorders
- Ocular Diseases and Behçet’s Syndrome
- Ocular Disorders and Treatments
- Retinal and Optic Conditions
- Ophthalmology and Visual Impairment Studies
- Traumatic Ocular and Foreign Body Injuries
- Tissue Engineering and Regenerative Medicine
- Immunotherapy and Immune Responses
- Inflammasome and immune disorders
- Herpesvirus Infections and Treatments
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Retinopathy of Prematurity Studies
- Retinal Diseases and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- NF-κB Signaling Pathways
- Electrospun Nanofibers in Biomedical Applications
- Retinal Development and Disorders
Shandong First Medical University
2016-2025
Shandong Eye Hospital
2016-2025
Academy of Medical Sciences
2021
Shandong Museum
2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2012-2020
Chinese Medical Association
2020
Shandong Eye Institute & Hospital
2006-2019
Qingdao University
2006-2019
Jinan University
2012-2014
University of Jinan
2013
Death of pancreatic β cells is a pathological hallmark type 1 diabetes (T1D). However, the molecular mechanisms cell death and its regulation are poorly understood. Here we describe unique regulatory pathway that comprises microRNA-21, target tumor suppressor PDCD4, upstream transcriptional activator nuclear factor-κB (NF-κB). In cells, c-Rel p65 NF-κB family activated mir21 gene promoter increased miR-21 RNA levels; in turn decreased level which able to induce through Bax apoptotic...
Keratoconus is a common ectatic corneal disorder in adolescents and young adults that can lead to progressive visual impairment or even legal blindness. Despite the high prevalence, its etiology not fully understood. In this study, we performed single-cell RNA sequencing (scRNA-Seq) analysis on 39,214 cells from central corneas of patients with keratoconus healthy individuals, define involvement each cell type during disease progression. We confirmed role stromal disease, where dysregulation...
Abstract Corneal substitutes with structural and compositional characteristics resembling those of natural corneas have attracted considerable attention. However, biomimicking the complex hierarchical organization corneal stroma is challenging. In this study, humanized stroma‐like adhesive patches (HCSPs) are prepared through a multi‐step process. First, polyethylene glycol diacrylate cast cured within decellularized porcine cornea (DPC) templates. The DPCs then enzymatically digested to...
Abstract A stable topical ophthalmic cyclosporine (CsA) formulation with good tolerance and high efficacy is still a desire in pharmaceutics clinics. This article describes the preparation of CsA containing nanomicelles using polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol (PVCL-PVA-PEG) graft copolymer. Both polymer itself were evaluated for cytotoxicity ocular irritation. The vitro uptake intracellular fate characterized. In vivo cornea permeation test performed 0.5 mg/mL...
Significance Mirabegron as a β3-adrenoceptor agonist is routinely prescribed drug for treating overactive bladder syndrome. However, the effect of this on off-targeted tissues and organs largely unknown. In study, we provide mechanistic insights mirabegron-triggered atherosclerosis in causing potential cardiovascular cerebrovascular diseases by activation brown fat. Given that mirabegron can induce fat adult human subjects, these findings are clinically relevant. Moreover, genetic mutations...
Human pluripotent stem cells (hPSCs) hold great promise for the treatment of various human diseases. However, their therapeutic benefits and mechanisms treating corneal endothelial dysfunction remain undefined. Here, we developed a regimen consisting combination hPSC-derived precursors (CEPs) with nicotinamide (NAM) effective dysfunction. In rabbit nonhuman primate models, intracameral injection CEPs NAM achieved long-term recovery clarity thickness, similar outcome cultured (CECs). The...
Abstract Pancreatic β cell dysfunction contributes to the pathogenesis of type 2 diabetes. MiR-21 has been shown be induced in islets glucose intolerant patients and diabetic mice. However, role miR-21 regulation pancreatic function remains largely elusive. In current study, we identify pathway by which regulates glucose-stimulated insulin secretion utilizing mice lacking their cells ( miR-21β KO). We find that KO develop intolerance due impaired secretion. Mechanistic studies reveal...
Abstract Corneal injectable hydrogels represent a promising alternative to alleviate donor shortage and simplify traditional surgeries. However, most focus on repairing focal corneal defects (≤3.5 mm) leave many clinical requirements unmet. Herein, novel ion‐activated bioadhesive hydrogel (IonBAH) is designed its long‐term performance of large (6 evaluated in rabbits for 6 months. The IonBAH dual‐network composed natural extracellular matrix peptide‐modified alginate, which enables desirable...
The repair of large-diameter corneal stroma defects is a major clinical problem. Although some studies have attempted to use hydrogels damage, most these can only be used for focal stromal that are ≤3.5 mm in diameter due poor hydrogel adhesion. Here, photocurable adhesive mimics the extracellular matrix (ECM) with regard composition repairing 6 mm-diameter rabbits investigated. This ECM-like rapidly cured after light exposure, high transmittance and good mechanical properties. More...
Purpose: To evaluate the complications and therapeutic effects of penetrating keratoplasty (PKP) in treatment corneal perforations fungal keratitis. Methods: The medical records patients who underwent PKP for keratitis at Shandong Eye Institute from January 1999 to December 2004 were retrospectively reviewed. Results: Fifty-two (52 eyes) included this study. Twenty eyes (38.5%) had graft rejection after primary PKP, 12 which medically treated, 8 secondary with 4 acquiring clear grafts....
The corneal endothelium is critical for maintaining clarity by mediating hydration through barrier and pump functions. Progressive loss of endothelial cells during aging has been associated with the development Fuchs dystrophy (FECD), one main causes cornea-related vision loss. mechanisms underlying FECD remain elusive. Single-cell RNA sequencing isolated healthy human corneas discovered 4 subpopulations distinctive signatures. Unsupervised clustering analysis uncovered nuclear enriched...
Abstract Immune rejection and side effects of long‐term administration immunosuppressants are the two major obstacles to allograft acceptance tolerance. The immunosuppressive extracellular vesicles (EVs)‐based approach has been proven be effective in treating autoimmune/inflammatory disorders. Herein, anti‐rejection advantage exosomes (Rapa‐Exo) from rapamycin‐conditioned myeloid‐derived suppressor cells (MDSCs) over (Exo‐Nor) untreated MDSCs is shown. exosomal small RNA sequencing...
To determine the therapeutic value of lamellar keratoplasty (LKP) in treatment fungal keratitis not curable by antifungal chemotherapy.Fifty-five patients, whom a diagnosis was confirmed microscopic analysis corneal scrapings or confocal microscopy, and who were cured topical oral medication, given LKP. After LKP, continued for 2 weeks with gradual tapering drugs. The excised recipient lamella used microbial culture histopathologic examination.Therapeutically beneficial results achieved 51...
To analyze leading indications for penetrating keratoplasty (PKP) in north China and changing trends them.We retrospectively reviewed the records of patients who underwent PKP at Shandong Eye Institute from January 1997 to December 2002. Infectious keratitis (fungal, bacterial, acanthamoeba), herpes simplex (HSK), corneal scarring, keratoconus, bullous keratopathy, regrafting, dystrophy degeneration, others were included PKP. Initial diagnoses causes regrafting recorded, as well related...
Purpose Macrophages have been shown to play a critical role in the wound healing process. In present study, of macrophages after autologous corneal transplantation was investigated by depleting local infiltrated macrophages. Methods Autologous model used induce repair Balb/c mice. were depleted sub-conjunctival injections clodronate-containing liposomes (Cl2MDP-LIP). The presence CD11b+ F4/80+ macrophages, α-smooth muscle actin+ (α-SMA+) myofibroblasts, CD31+ vascular endothelial cells and...
To investigate the morphological changes of meibomian glands in patients with type 2 diabetes mellitus (DM).Of 118 eyes (118 patients) DM (DM group) and 100 control subjects (control were enrolled. After completing an ocular surface disease index (OSDI) questionnaire, non-invasive tear film break-up time (NI-BUT) structure (MGs, meibography) assessed by Keratograph 5M system. Partial or complete loss MG was scored for each eyelid from grade 0 (no loss) to 3 (lost area >2/3 total area), which...
The corneal epithelium plays important roles in the maintenance of transparency for good vision, and acts as a protective barrier against foreign insults. Structural functional changes with aging have been documented. Here we found that transforming growth factor-β (TGF-β) is highly expressed elderly donor epithelium, are senescence-associated genes, such p16 p21. In human epithelial cell (HCEC) models, TGF-β induces cellular senescence, characterized by increased SA-β-gal positive cells...