Until recently, the studies of pathogenetic mechanisms different diseases were focused on evaluation major cell populations, changes in their quantitative and qualitative characteristics, such as gene expression profile, secreted factors, surface markers, etc. Nowadays, minor subpopulations, low amounts peripheral blood are considerable interest. These cells may function variable ways, by polarizing immune response, or participating regulation reactions. One subpopulations is presented...

Currently, bronchial asthma (BA) and atopic dermatitis (AD) are among the most common allergic diseases. According to available data, worldwide incidence of is about 260 million people. Prevalence BA in adult population 6.9%, 10% children adolescents. AD occurs approximately 10-30% 2-10% adults developed countries. Every year, a steady increase noted, but pathogenesis these diseases remains poorly known. Recently, research being devoted studies interactions between immune cells molecules...

Multiple myeloma (MM) is a B-cell malignant tumor, the morphological substrate of which are plasma cells that produce monoclonal immunoglobulin. This one most common tumors lymphoid origin. It known during oncogenesis, immune balance shifts towards suppression antitumor response. Innate (ILC) key factors influencing said balance. study aimed to assess features ILC in MM patients. The peripheral blood levels ILC1, ILC2, and ILC3, as well expression HLA-DR on 2, were measured with help flow...

Cucurbiturils are a family of macrocyclic oligomers capable forming host–guest complexes with various molecules. Due to noncovalent binding drug molecules and low toxicity, cucurbiturils has been extensively investigated as potential carriers for delivery. However, the immune system’s interactions different carriers, including cucurbiturils, still under investigation. In this study, we focused on cucurbiturils’ immunosafety immunomodulation properties in vivo. We measured blood counts...

Currently, cucurbiturils are being actively researched all over the world. Research is focused on ways of improving solubility and selectivity cucurbiturils, increasing stability complexes with other particles in various media enhancing their ability to bind release substances. The most significant area our research assessment safety, studying biological properties synergistic effects during complexation drugs. In this article, hemocompatibility erythrocytes leukocytes was investigated. We...

Short regulatory oligonucleotides are considered prospective tools for immunotherapy. However, they require an adequate carrier to deliver potential therapeutics into immune cells. Herein, we explore the of polycationic dendrimers as carriers microRNAs in peripheral blood mononuclear cells healthy donors. As oligonucleotide cargo, use a synthetic mimic and inhibitor miR-155, important factor development functioning immunocompetent Dendrimers bind low-cytotoxic polyelectrolyte complexes that...

Abstract The cellular uptake of drug carriers to the cytosol a specific cell remains challenging, and non‐classical supramolecular strategy is motivated. Here, we select model host–guest complex in which diamino‐viologen (1,1′‐bis(4‐aminophenyl)‐[4,4′‐bipyridine]−1,1′‐diium dichloride [VG]) fluorescent tag was engulfed by cucurbit[8]uril (CB8) covalently linked alginate polysaccharides (alginic acid [ALG]) as modified vehicle. When adsorbed on ALG surface, encapsulation VG first confirmed...

Reactive oxygen species (ROS) are highly reactive chemical molecules containing oxygen. ROS play an important role in signaling and cell homeostasis at low moderate concentrations. could be a cause of damage to proteins, nucleic acids, lipids, membranes organelles high There lot cells that can produce maintain functional activity. It is known metal nanoparticles increase production cells. However, the effect cucurbiturils on still unknown. In our study, we evaluated by immune (T-,...

Tumors are a leading pathology in the population. Chemotherapy cannot provide adequately and effectively to cure patients. Some medicine, such as cytostatic, characterized by wide range of side effects resistance solid tumors chemotherapy these medicines. In recent research, mechanisms action cytotoxic agents have been described, most appropriate causes suggested. Drug delivery system based on Cucurbit[7]uril (CB[7]) was used minimize overcome resistance. CB[7] has ability form host-guest...

. Cucurbiturils are a family of macrocyclic oligomers capable forming host-guest complexes with various molecules. Due to noncovalent binding drug molecules and low toxicity, cucurbiturils has been extensively investigated as potential carrier for delivery. However, the immune system interactions different carriers, including cucurbiturils, still under investigation. In this study, we focused on immunosafety immunomodulation properties in vivo. We measured blood counts, lymphocyte...

HLA-E is a minor understudied non-classical HLA genes. transcription revealed in many cell types, especially, immune cells, e.g., T and B cells monocytes, macrophages. In this work, we evaluated expression of on CD8+, CD4+ CD14+ conditionallу healthy donors the patients with bronchial asthma (BA) rheumatoid arthritis (RA). Peripheral blood mononuclears (PBMNC) were used as initial biomaterial. PBMNC from RA (n = 15), BA 11) separated peripheral Ficoll-Urographin density gradient (1.077...

In this work we assessed the surface expression of HLA-E in patients with bronchial asthma comparison a group healthy donors. We showed that BA had lower level on CD8+ cytotoxic T cells and CD14+ than conditionally

In this work we studied the ratio of ILC in patients with RA. We have shown that early RA balance change towards 2, and during development on contrary 1. imbalance may contribute to To better understand pathogenesis RA, further study subpopulation composition, phenotypic functional characteristics disease is necessary.

Autoimmune diseases currently take a leading place in terms of frequency occurrence the population, among which 1 percent is occupied by rheumatoid arthritis (RA). Remission this type disease extremely rare and requires constant use pharmacotherapy. Studying pathogenesis RA necessary to study search for new drug targets. It known that T helpers (Th) Th17 are involved development RA. However, some researchers suggest ILCs play role “innate analogues” Th, due fact subpopulation synthesizes...

The cellular uptake of drug carriers to the cytosol a specific cell remains challenging, and non-classical supramolecular strategy is motivated. Here, we select model host-guest complex in which diamino-viologen (VG) fluorescent tag was engulfed by cucurbit[8]uril (CB8) covalently linked alginate polysaccharides (ALG) as modified vehicle. When adsorbed on ALG surface, encapsulation VG first confirmed utilizing FTIR NMR spectroscopic methods. Solid optical measurements (DRS, PL, TRPL)...

Currently, rheumatoid arthritis (RA) is considered among the most common autoimmune diseases worldwide, being associated with progressing disability, systemic organ and tissue lesions, as well social economic losses for state. Studies of innate lymphoid cells (ILS) seems to be actual significant when studying development inflammation in RA, particular, issues cell plasticity. ILC represent resident that display functional diversity, like T cells. Moreover, regulate orientation immune...

Background. Due to their very small size, nanomaterials, in particular cucurbiturils, have unique physical and chemical properties that find application medicine. However, the toxicity of cucurbiturils is not fully understood; particular, we are interested immunological safety use. One mechanisms nanotoxicity formation reactive oxygen species (ROS) by macrophages neutrophils. Hyperproduction ROS can lead oxidative stress further damage cell DNA with loss physiological function development...