A5045562535- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Estrogen and related hormone effects
- Cancer-related gene regulation
- RNA Interference and Gene Delivery
- Microtubule and mitosis dynamics
- Breast Cancer Treatment Studies
- RNA modifications and cancer
- Cancer therapeutics and mechanisms
- Cancer Genomics and Diagnostics
- Carcinogens and Genotoxicity Assessment
- Click Chemistry and Applications
- Synthesis and Characterization of Heterocyclic Compounds
- DNA and Nucleic Acid Chemistry
- Nanoplatforms for cancer theranostics
- Cell death mechanisms and regulation
- RNA Research and Splicing
- Melanoma and MAPK Pathways
- Genomics and Chromatin Dynamics
- Peptidase Inhibition and Analysis
- Advanced biosensing and bioanalysis techniques
- Radiopharmaceutical Chemistry and Applications
The Barbara Ann Karmanos Cancer Institute
2014-2025
Wayne State University
2015-2025
Cardiff University
2013-2016
Zagazig University
2013
Indiana University School of Medicine
2013
Eugene Applebaum College of Pharmacy and Health Sciences
2007-2008
University of Minnesota
2008
University of Manitoba
2007
University of Nebraska Medical Center
2007
Molecular Oncology (United States)
1994
Nanoparticles enhance the therapeutic efficacy of an encapsulated drug by increasing and sustaining delivery inside cell. We have previously demonstrated that Aerosol OT (AOT)–alginate nanoparticles, a novel formulation developed recently in our laboratory, significantly drugs like doxorubicin drug-sensitive tumor cells. The purpose this study is to evaluate potential AOT–alginate nanoparticles drug-resistant cells overexpressing efflux transporter, P-glycoprotein (P-gp). were formulated...
Photodynamic therapy (PDT) is a promising treatment modality for cancer. PDT based on the concept that photosensitizers, when exposed to light of specific wavelength, generate cytotoxic reactive oxygen species (ROS) capable killing tumor cells. The effectiveness has been limited in part by lack photosensitizers accumulate sufficiently cells and poor yield ROS from existing photosensitizers. In this report, we investigated whether aerosol OT-alginate nanoparticles can be used as carrier...
Background : DDT and polychlorinated biphenyls (PCBs), which are widespread in the ecosystem, can mimic estrogenmediated cell activities. Thus, they potentially interfere with many physiologic processes. We compared effects of organochlorines belonging to PCB families, alone combination, for their ability influence estrogen receptor-mediated activities preneoplastic breast epithelial cells cancer cells. Methods Multiple assay systems requiring functional receptor were employed test...
Abstract Cancer progression represents an evolutionary process where overall genome level changes reflect system instability and serve as a driving force for evolving new systems. To illustrate this principle it must be demonstrated that karyotypic heterogeneity (population diversity) directly contributes to tumorigenicity. Five well characterized in vitro tumor models representing various types of cancers were selected such analysis. The tumorigenicity each model has been linked different...
Abstract Ocimum sp. is a traditionally used medicinal herb, which shows anti‐oxidant, anti‐carcinogenic, radio‐protective and free radical scavenging properties. So far no detailed studies have been reported on its effects human cancers. Thus, we analyzed breast cancer utilizing in vitro vivo methodologies. Aqueous extracts were prepared from the mature leaves of gratissimum (OG) cultivated devoid pesticides. Tumor progression angiogenesis related processes like chemotaxis, proliferation,...
Chromosome fragmentation (C-Frag) is a newly identified MCD (mitotic cell death), distinct from apoptosis and MC catastrophe). As different molecular mechanisms can induce C-Frag, we hypothesize that the general mechanism of its induction system response to cellular stress. A clear link between C-Frag diverse stresses generated an array shown. Centrosome amplification, which also linked stress, shown occur in association with C-Frag. This led new model showing common, MCD. Specifically,...
Abstract Protein ubiquitination is important for cell signaling, DNA repair, and proteasomal degradation, it not surprising that alterations in occur frequently cancer. Ubiquitin-conjugating enzymes (E2) mediate by selective interactions with ubiquitin-activating (E1) ubiquitin ligase (E3) enzymes, thus E2 small molecule inhibitor (SMI) will provide specificity unattainable proteasome inhibitors. Here we describe synthesis functional characterization of the first SMIs human Rad6B, a...
Vemurafenib constitutes an important therapeutic for BRAFV600 mutant melanomas, but despite high initial response rates, resistance to BRAF and MEK inhibitors quickly develops. Here, we performed integrative analysis of metabolomic consequences transcriptome alterations uncover mechanisms involved in adaptive vemurafenib (VemR) development their relationship with tolerance thresholds. We developed BRAFV600E isogenic models VemR utilizing M14 A2058 lines, patient-derived melanomas V600E or...
Abstract Mutations in β-catenin or other Wnt pathway components that cause accumulation occur rarely breast cancer. However, there is some evidence of protein a subset tumors. We have recently shown Rad6B, an ubiquitin-conjugating enzyme, transcriptional target β-catenin/TCF. Here, we show forced Rad6B overexpression MCF10A cells induces accumulation, which despite being ubiquitinated stable and transcriptionally active. A similar relationship between ubiquitination, activity was found WS-15...
Ocimum genus (a.k.a holy basil or tulsi) is a dietary herb used for its multiple beneficial pharmacologic properties including anti-cancer activity. Here we show that crude extract of gratissimum (OG) and hydrophobic hydrophilic fractions (HB HL) differentially inhibit breast cancer cell chemotaxis chemoinvasion in vitro retard tumor growth temporal progression MCF10ADCIS.com xenografts, model human comedo-ductal carcinoma situ (comedo-DCIS). OG-induced inhibition was associated with...