Summary The plant family 1 UDP‐glycosyltransferases (UGTs) are the biggest GT in plants, which responsible for transferring sugar moieties onto a variety of small molecules, and control many metabolic processes; however, their physiological significance planta is largely unknown. Here, we revealed that two Arabidopsis glycosyltransferase genes, UGT79B2 UGT79B3 , could be strongly induced by various abiotic stresses, including cold, salt drought stresses. Overexpression / B3 significantly...

Cytokinins are plant hormones that can be glucosylated to form O-glucosides and N-glucosides. The glycoconjugates inactive thought play a role in homeostasis of the hormones. Although O-glucosyltransferases have been identified recognize cytokinins, enzymes involved N-glucosylation not even though process has recognized for many years. This study utilizes screening strategy which 105 recombinant glycosyltransferases (UGTs) Arabidopsis analyzed catalytic activity toward classical cytokinins:...

Regioselectivity of glycosyltransferases offers an important means to overcome the limitations chemical synthesis small molecule glycosides. In this study we explore a large multigene family UDP-glucose:glycosyltransferases Arabidopsis for their potential as novel biocatalysts in vitro and whole-cell catalysis. We used quercetin substrate because flavonol its glycosides have medicinal properties metabolite provides complex structure regioselective glucosylation. analyzed activity 91...

Glycosylation of monolignols has been found to be widespread in land plants since the 1970s. However, whether monolignol glycosylation is crucial for cell wall lignification and how it exerts effects are still unknown. Here, we report identification a mutant ugt72b1 showing aggravated ectopic floral stems along with arrested growth anthocyanin accumulation. Histochemical assays thioacidolysis analysis confirmed enhanced increased lignin biosynthesis mutant. The loss UDP-glycosyltransferase...

Salicylic acid (SA) plays a crucial role in plant innate immunity. The deployment of SA-associated immune responses is primarily affected by SA concentration, which determined balance between biosynthesis and catabolism. However, the mechanisms regulating homeostasis are poorly understood. In this study, we characterized unique UDP-glycosyltransferase, UGT76D1, an important associated Arabidopsis (

Plant systemic acquired resistance (SAR) provides an efficient broad-spectrum immune response to pathogens. SAR involves mobile signal molecules that are generated by infected tissues and transported tissues. Methyl salicylate (MeSA), a molecule can be converted salicylic acid (SA), is essential for establishing SAR, particularly under short period of exposure light after pathogen infection. Thus, the control MeSA homeostasis important optimal response. Here, we characterized uridine...

UDP-glycosyltransferases (UGTs) constitute the largest glycosyltransferase family in plant kingdom. They are responsible for transferring sugar moieties onto various small molecules to control many metabolic processes. However, their physiological significance plants is largely unknown. Here, we revealed function and mechanism of two Arabidopsis UGT genes, UGT73C3 UGT73C4, which can be strongly induced by Pseudomonas syringae pv. tomato DC3000. Their overexpression significantly enhanced...

Abiotic stresses greatly influence plant growth and productivity. While glycosyltransferases are widely distributed in kingdom, their biological roles response to abiotic largely unknown. In this study, a novel Arabidopsis glycosyltransferase gene UGT85A5 was identified as significantly induced by salt stress. Ectopic expression of tobacco enhanced the stress tolerance transgenic plants. There were higher seed germination rates, better less chlorophyll loss lines compared wild type plants...

Cytokinins are a class of phytohormones that play crucial role in plant growth and development. The gene UGT76C2 encoding cytokinin N-glucosyltransferase Arabidopsis thaliana has been previously identified. To determine the planta metabolism response, we analyzed phenotypes its loss-of-function mutant (ugt76c2) overexpressors. accumulation level N-glucosides was significantly decreased ugt76c2, but substantially increased overexpressors compared with wild type. When treated exogenously...

Although plant glycosyltransferases are thought to play important roles in growth and interaction with the environment, little is known about their physiological for most members of glycosyltransferase family. We cloned characterised an Arabidopsis gene, UGT76E11. Its vivo effects on flavonoid accumulation tolerance abiotic stresses were investigated. The UGT76E11 gene was up-regulated transcription expression under stress conditions salinity, drought H2 O2 treatment. Transgenic plants...

Significance Auxin plays a crucial role in the life cycle of plants. In plant cells, auxin concentration must be closely controlled for optimal responses. However, underlying mechanism governing homeostasis remains largely unknown. this study, UDP-glycosyltransferase (UGT76F1) has been identified as involved by glucosylation major precursor (IPyA) to form IPyA glucose conjugates (IPyA-Glc). This process is negatively regulated transcription factor PIF4 and vital light- temperature-dependent...

• Family 1 glycosyltransferases comprise the greatest number of found in plants. The widespread occurrence and diversity glycosides throughout plant kingdom underscore importance these glycosyltransferases. Here, we describe identification characterization a late-flowering Arabidopsis (Arabidopsis thaliana) mutant, which putative family glycosyltransferase gene, UGT87A2, was disrupted. role possible mechanism UGT87A2 regulation flowering were analyzed by molecular, genetic cellular...

Nowadays, the plant family 1 glycosyltransferases (UGTs) are attracting more and attention since members of this can improve properties secondary metabolites have significantly enriched chemical species in plants. Over past decade, most studies on UGTs been conducted Arabidopsis thaliana they were proved to play diverse roles during life cycle. The Zea mays (maize) GT1 comprises a large number UDP-glycosyltransferase (UGT) members. However, their enzyme activities biological functions rarely...

Recent studies have shown that global metabolic reprogramming is a common event in plant innate immunity; however, the relevant molecular mechanisms remain largely unknown. Here, we identified pathogen-induced glycosyltransferase, UGT73C7, plays critical role Arabidopsis disease resistance through mediating redirection of phenylpropanoid pathway. Loss UGT73C7 function resulted significantly decreased to Pseudomonas syringae pv. tomato DC3000, whereas constitutive overexpression led an...

Drought is one of the most important environmental constraints affecting plant growth and development ultimately leads to yield loss. Uridine diphosphate (UDP)-dependent glycosyltransferases (UGTs) are believed play key roles in coping with stresses. In rice, it estimated that there more than 200 UGT genes. However, them have not been identified as their physiological significance. this study, we reported characterization a putative glycosyltransferase gene UGT85E1 rice. significantly...