Increased aortic stiffness, an important feature of many vascular diseases, eg, aging, hypertension, atherosclerosis, and aneurysms, is assumed because changes in extracellular matrix (ECM).We tested the hypothesis that mechanisms also involve intrinsic stiffening smooth muscle cells (VSMCs).Stiffness was measured vitro both by atomic force microscopy (AFM) a reconstituted tissue model, using VSMCs from aorta young versus old male monkeys (Macaca fascicularis) (n=7/group), where stiffness...

To seek evidence that the nonhuman primate arterial wall, as it ages in absence of atherosclerosis, exhibits alterations pathways are involved pathogenesis experimental we assessed aortic matrix metalloproteinase-2 (MMP-2) and its regulators, ie, membrane type-1 metalloproteinase (MT1-MMP) tissue inhibitor (TIMP-2), expression angiotensin II (Ang II), angiotensin-converting enzyme (ACE), chymase young (6.4+/-0.7 years) old (20.0+/-1.9 male monkeys. With advancing age, (1) intimal thickness...

Abstract —To determine the effects of aging on vasoactivity in a primate model ( Macaca fascicularis ), 13 young male monkeys (aged 7.1±0.4 years) and 9 old 19.8±0.6 were chronically instrumented for measurement left ventricular aortic pressures cardiac output. Total cholesterol, triglyceride, fasting blood sugar levels not different between 2 groups. There no significant differences baseline mean pressure total peripheral resistance (TPR) versus monkeys. TPR fell less P <0.05) with...

SUMMARY Severe thrombocytopenia and increased vascular permeability are two major characteristics of dengue haemorrhagic fever (DHF). To develop a better understanding the roles platelet-associated IgG (PAIgG) IgM (PAIgM) in inducing its severity disease patients with secondary virus infection, relationship between PAIgG or PAIgM levels as well was examined 78 acute phase infection prospective hospital-based study. The decrease platelet count during recovered significantly convalescent...

Background— Our hypothesis was that the changes in vascular properties responsible for aortic stiffness with aging would be greater old male monkeys than female monkeys. Methods and Results— We analyzed effects of gender differences on vivo measurements pressure diameter extracellular matrix thoracic aorta young adult (age, 6.6±0.5 years) versus 21.2±0.2 ( Macaca fascicularis ). Aortic stiffness, as represented by strain elastic modulus (Ep), increased more (5.08±0.81; P <0.01) females...

The mechanisms of thrombocytopenia and platelet phagocytosis in dengue illness are not fully understood.A prospective hospital-based study was conducted to examine the relationships between counts, serum thrombopoietin (TPO) levels, apoptosis 81 patients with secondary virus (DV) infections 38 healthy volunteers. cultured platelets after exposure DV were also examined.Platelet apoptosis, phagocytosis, TPO levels increased significantly during acute early convalescence phases compared...

To demonstrate the differences of clinical features and hematologic abnormalities between dengue fever (DF) hemorrhagic (DHF), 359 pediatric patients admitted St. Luke's Medical Center in Quezon City, 1999 2001 Metro Manila, adjoining provinces Philippines, with a laboratory-confirmed virus infection were evaluated. One third had DHF, most these without shock. Restlessness, epistaxis, abdominal pain more associated DHF. The platelet count was significantly lower DHF group than DF before...

The goal of this investigation was to determine the distribution myocardial apoptosis in myocytes and nonmyocytes primates patients with heart failure (HF). Almost all clinical cardiologists cardiovascular investigators believe that myocyte is considered be a cardinal sign HF major factor its pathogenesis. However, knowledge 75% number cells are nonmyocytes, it important whether occurring or nonmyocytes. We studied both nonhuman primate model chronic HF, induced by rapid pacing 2-6 mo after...

Abstract X‐linked dystonia–parkinsonism (XDP), or Lubag syndrome, is known to cause progressive dystonia, with without parkinsonism, among Filipino male adults maternal roots from the Philippine island of Panay. We present cinematographic material 11 cases carrying XDP haplotypes who manifest a wide spectrum movement disorders, including tremor, myoclonus, chorea, and myorhythmia. Because overlapping features, patients are commonly misdiagnosed as idiopathic essential Parkinson's disease,...

<h3>Background</h3> X-linked dystonia-parkinsonism (XDP) or "lubag" is an recessive disorder that afflicts Filipino men, and rarely, women. Genetic confirmation performed through haplotyping detection of disease-specific changes in the<i>DYT3</i>gene. <h3>Objective</h3> To describe the phenotypes molecular data 8 symptomatic female patients with XDP from 5 kindreds. <h3>Methods</h3> Case series. <h3>Results</h3> The average age onset symptoms was 52 years (range, 26-75 years). Six had...

Because most cases of secondary dengue virus infection are associated with an increased level platelet-associated IgG, a high dose intravenous immunoglobulin (IVIG) may have effect on the development severe thrombocytopenia in this disease. A randomized, controlled study was conducted two treatment groups consisting group (n = 15) and non-treatment (non-IVIG) 16) to determine whether IVIG is effective hastening recovery from patients infection. No significant difference found baseline...

Abstract In Myanmar, dengue fever (DF)/dengue hemorrhagic (DHF) is one of the leading causes morbidity and mortality among children. From Pyinmana Hospital in 2004 Mandalay Children 2006, 160 patients diagnosed clinically to have DHF/dengue shock syndrome (DSS) were examined for immunoglobulin M (IgM) IgG levels. A focus reduction neutralization test was also used determine primary or secondary virus (DENV) infection. By using IgM‐capture ELISA, 139 cases confirmed as DENV infections. Of...

In 2005, we isolated a new species of virus from mosquitoes in the Philippines. The virion was elliptical shape and had short single projection. named Tanay (TANAV) after locality which it found. TANAV genomic RNA 9562 nt+poly-A positive strand, polycistronic. longest ORF contained putative RNA-dependent polymerase (RdRP); however, conserved motifs RdRP were permuted. phylogenetically close to Negevirus, recently proposed taxon viruses haemophagic insects, some plant viruses, such as citrus...

Serum samples from 54 monkeys were collected healthy individuals in a monkey farm Luzon island, Philippines, 1999, and examined by IgM-capture ELISA indirect IgG for the presence of dengue (DEN), Japanese encephalitis (JE) chikungunya (CHIK) viruses. The positive rates IgM 3.7, 35.2 14.8% against DEN, JE CHIK, respectively. Higher obtained when was used: 100% flaviviruses (JE or DEN) 59.3% CHIK virus. results indicate high prevalence flavivirus infections such as lesser virus infections,...

The relationship between the percent phagocytosis of platelets by differentiated THP-1 cells was examined using flowcytometry and peripheral platelet counts as well platelet-associated IgG (PAIgG) in 36 patients with secondary dengue virus (DV) infections. levels PAIgG were significantly increased these during acute phase compared healthy volunteers. found decreased convalescent phase. An inverse correlation count ( P = 0.011) 0.041) among No found, however, PAIgG. Our present data suggest...

Although the public health impact of dengue is increasing rapidly, mechanism thrombocytopenia in this disease remains unknown. To elucidate mechanism, relationship between platelet-associated IgG (PAIgG) and platelet count 53 patients acute phase secondary virus infection was investigated a prospective-hospital-based study. A significant inverse correlation two parameters found these patients, while no observed healthy volunteers. The low baseline counts during 12 with significantly...

The pre-membrane (prM) and envelope (E) genes of 41 viruses isolated from dengue fever (DF), hemorrhagic (DHF), shock syndrome (DSS) patients 1995 to 2002 were sequenced determine the genetic variability 2 (DENV 2) in Philippines. sequence data compared with a global sample DENV obtained GenBank. Phylogenetic analysis revealed that two distinct genotypes, Asian Cosmopolitan, are currently circulating locally, each potential cause severe disease. After initial isolation 1998, Cosmopolitan...

To establish a new method for the diagnosis of dengue secondary infection, 187 serum samples from patients with 40 primary and 44 healthy volunteers were tested using IgG indirect enzyme-linked immunosorbent assay (DEN ELISA). The results test compared those hemagglutination inhibition HI) test, which has been recommended as gold standard by World Health Organization (WHO, 1997). Japanese encephalitis ELISA (JE ELISA) was also performed to measure anti-flavivirus IgG, cross-reacts virus,...

Although increased vascular stiffness is more prominent in aging males than females, and are prone to disease with aging, no study has investigated the genes potentially responsible for sex differences aging. We tested hypothesis that transcriptional adaptation differs females using a monkey model, which not only physiologically phylogenetically closer humans commonly studied rodent models but also afflicted most common forms of accompany process humans, e.g., atherosclerosis, hypertension,...

SUMMARY Chikungunya virus (CHIKV) and Ross River (RRV) of the genus Alphavirus , family Togaviridae are mainly transmitted by Aedes mosquitoes symptoms they cause in patients similar to dengue. A chikungunya (CHIK) outbreak re-emerged several Asian countries during 2005–2006. This study aimed clarify prevalence CHIKV infection suspected dengue six South Asia Southeast Asia. Seven hundred forty-eight serum samples were from dengue-suspected Asia, 52 Fiji. The analysed IgM capture ELISA, IgG...