A5059484855- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Biology and Fertility
- Developmental Biology and Gene Regulation
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Legume Nitrogen Fixing Symbiosis
- Advanced Breast Cancer Therapies
- Cancer Genomics and Diagnostics
- Circadian rhythm and melatonin
- Bacteriophages and microbial interactions
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- CRISPR and Genetic Engineering
- Genetics, Bioinformatics, and Biomedical Research
- Chromosomal and Genetic Variations
- Nutrition, Genetics, and Disease
- Plant Molecular Biology Research
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- Cancer-related Molecular Pathways
- Muscle Physiology and Disorders
Fred Hutch Cancer Center
2004-2019
Cancer Research Center
2007
Instituto Gulbenkian de Ciência
2005-2007
Howard Hughes Medical Institute
2004-2007
Universidade Nova de Lisboa
2004
The four-cell C. elegans embryo contains two sister cells called ABa and ABp that initially have equivalent abilities to produce ectodermal cell types. Multiple Notch-mediated interactions occur during the early divisions diversify descendants. first interaction determines pattern of types produced by ABp. second induces granddaughters become mesodermal precursors. We show T-box transcription factors TBX-37 TBX-38 are essential for induction, these expressed in ABa, but not ABp, provide...
The Notch signaling pathway is involved in a wide variety of cell-fate decisions during development. diverse behavior Notch-activated cells thought to depend on tissue- or cell-type-specific transcription factors, yet the identities such factors and mechanism cooperation with are largely unknown. We identify here an enhancer promoter ref-1, C. elegans target, which promotes Notch-dependent expression mesodermal endodermal cells. contains predicted binding sites for transcriptional effector...
RNA polymerase sigma factor sigma(F) initiates the prespore-specific program of gene expression during Bacillus subtilis sporulation. governs transcription spoIIIG, encoding late regulator sigma(G). However, spoIIIG is delayed relative to other genes under control sigma(F), and after synthesis, sigma(G) initially kept in an inactive form. Activation requires complete engulfment prespore by mother cell spoIIIA spoIIIJ loci. We screened for random mutations that bypassed requirement activation...
The transcriptional mechanisms that allow neural stem cells (NSC) to balance self-renewal with differentiation are not well understood. Employing an in vivo RNAi screen we identify here NSC-TAFs, a subset of nine TATA-binding protein associated factors (TAFs), as NSC identity genes Drosophila We found depletion NSC-TAFs results decreased clone size, reduced proliferation, defective cell polarity and increased hypersensitivity cycle perturbation, without affecting survival. Integrated gene...
Abstract The transcriptional mechanisms that allow neural stem cells (NSC) to balance self-renewal with differentiation are not well understood. Employing an in vivo RNAi screen we identify here NSC-TAFs, a subset of nine TATA-binding protein associated factors (TAFs), as NSC identity genes Drosophila . We found depletion NSC-TAFs results decreased clone size, reduced proliferation, defective cell polarity and increased hypersensitivity cycle perturbation, without affecting survival....