A5034570224- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- Electrospun Nanofibers in Biomedical Applications
- Glycosylation and Glycoproteins Research
- Reconstructive Surgery and Microvascular Techniques
- Graphene and Nanomaterials Applications
- Wound Healing and Treatments
- Animal Genetics and Reproduction
- Antiplatelet Therapy and Cardiovascular Diseases
- Silk-based biomaterials and applications
- Edcuational Technology Systems
- Brain Tumor Detection and Classification
- Bone Tissue Engineering Materials
- Virus-based gene therapy research
- Scientific and Engineering Research Topics
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Biopolymer Synthesis and Applications
Universitas Kristen Indonesia
2019-2023
University of Indonesia
2014-2021
Background: Adipose-derived stem cells (ADSCs) improve wound healing owing to their angiogenic potency. However, the production of large quantities ADSCs for clinical applications is laborious. In this study, efficacy stromal vascular fraction (SVF; non-expanded ADSCs) combined with platelet-rich plasma (PRP) which contains abundant growth factors, was evaluated using an animal model. Methods: PRP from venous blood and SVF lipoaspirates were harvested six donors. PRP, SVF, + saline solution...
Background . Stromal vascular fraction (SVF) therapy has been performed over the past six years to treat 421 patients by our group in five clinical centers. Autologous SVF, which is a substance containing stem cells, was isolated from lipoaspirate, mixed with platelet-rich plasma (PRP), and administered degenerative diseases, autoimmune trauma, aging, other diseases unknown etiology. This study aimed determine safety of SVF PRP that were given through infusion, spinal, intra-articular...
Introduction: Hyperglycemia in diabetic patients induces elevated pro-inflammatory cytokine production, resulting cellular damage, which may affect the regenerative function of mesenchymal stem cells (MSCs), such as adipose-derived (ADSCs). Identifying effect diabetes on ADSCs and optimization culture conditions is therefore an important starting point for application autologous to improve clinicial outcomes. The aim this study was investigate that cultured low-glucose anti-oxidant-serum...
Autologous transplantations, the gold standard, did not meet sufficient health tissue coverage area for cartilage damage treatments. The field of engineering offers a promising alternative to fulfill this limitation by growing patient own cells on biomaterials through culture, reconstructed into new tissue, and implanted injury area. To support regeneration, biocompatible, biodegradable, high strength silk fibroin (SF) was proposed in study as scaffold materials. In research, direct...
Background: Reprogrammed cell therapy has not been applied for clinical purposes due to the malignancy issue. The aim of this study was design recombinant vector transcription factors and analyze effectiveness cell-penetrating peptide delivering system human primary fibroblast transfection avoid issue.Materials methods: constructions CCAT/enhancer binding protein alpha (CEBPA), hepatocyte nuclear factor 4 (HNF4A), receptor subfamily 1 group I member 2 (NR1I2) were confirmed with DNA...
Background: One of the problems encountered in stromal vascular fraction (SVF) cells therapy as a regenerative medicine is finding an effective and efficient method adipose tissue processing. In this study, we try to improve whole processing so it not only generates high yield SVF, but also could saving cost time. Methods: Each 30 ml subcutaneous from twelve patients separated into three groups treatments follows: Group A treated with trypsin-EDTA, B recombinant enzyme, C commercial kit. The...
Osteogenic differentiation from Mesenchymal Stem Cell (MSC) to osteoblast has a clinical significance which is very important for treating bone injuries, in the form of femoral fractures with most cases Indonesia. Various studies have been conducted find best scaffold that can improve osteogenic differentiation, one development hybrid made natural biomaterials extracellular matrix, and synthetic biomaterials. The discovery not only focused on source but also requires optimization method...
The endogenous HIV-1 vaccine based on Gag protein is expected to stimulate the immune response of CD8+ T cells (cytotoxic). that has been produced by E.coli prokaryote system an exogenous antigen. fusion VP22 deliver antigen into cytoplasm cell, observed eGFP markers. Sequences (114 pb), GagHIV-1 (1506 and (733 pb) were inserted pQE80L, respectively. recombinant was expressed in purified Ni-NTA method. Antigen delivery fused with fluorescence confocal microscopy. plasmid constructs...