A5065479618- Folate and B Vitamins Research
- Porphyrin Metabolism and Disorders
- Microbial bioremediation and biosurfactants
- Aerospace and Aviation Technology
- Biochemical and Molecular Research
- Computational Fluid Dynamics and Aerodynamics
- Microbial metabolism and enzyme function
- Advanced Aircraft Design and Technologies
- Carcinogens and Genotoxicity Assessment
- Wastewater Treatment and Nitrogen Removal
- Metal-Catalyzed Oxygenation Mechanisms
- Body Image and Dysmorphia Studies
- Epigenetics and DNA Methylation
- Iron Metabolism and Disorders
- Concrete and Cement Materials Research
- Radiation Effects and Dosimetry
- Metalloenzymes and iron-sulfur proteins
- Rocket and propulsion systems research
- Adventure Sports and Sensation Seeking
- Effects and risks of endocrine disrupting chemicals
- Metabolism and Genetic Disorders
- Microbial Community Ecology and Physiology
- Aerospace Engineering and Energy Systems
- Amino Acid Enzymes and Metabolism
- Enzyme function and inhibition
The University of Texas at Austin
2024-2025
Applied Research Laboratories, The University of Texas at Austin
2024-2025
Ernst Strüngmann Institute for Neuroscience
2021-2024
Society for Neuroscience
2024
King's College London
2020-2022
Lurie Children's Hospital
2019-2021
Northwestern University
2016-2019
Royal London Hospital
2006-2018
Barts Health NHS Trust
2018
University of Leeds
1965-2016
Ancient Roman syntheses of Al-tobermorite in a 2000-year-old concrete block submerged the Bay Pozzuoli (Baianus Sinus), near Naples, have unique aluminum-rich and silica-poor compositions relative to hydrothermal geological occurrences. In relict lime clasts, crystals calcium contents that are similar ideal tobermorite, 33 35 wt%, but low-silica contents, 39 40 reflect Al 3+ substitution for Si 4+ Q 2 (1Al), 3 (2 Al) tetrahedral chain branching sites. The has double silicate structure with...
N5-Methyltetrahydrofolate-homocysteine transmethylase was isolated in millimicromole quantities (ZQO-fold purified) from an extract of 2 kg Escherichia coli B. Although
Abstract Leucine transaminase was purified extensively from pig heart muscle. It appeared to be nearly homogeneous by the criteria of both starch gel and free boundary electrophoresis ultracentrifugation. The molecular weight found 75,000. Purified leucine is yellow, our best preparations contained 1 mole bound pyridoxal phosphate per 75,000 g protein. spectrum enzyme has absorption maxima at 414 mµ 326 in addition characteristic 280-mµ peak. Addition an amino acid substrate shifted 414-mµ...
Abstract A simple colorimetric assay for measuring leucine-α-ketoglutarate transamination is described. It reproducible, sensitive, and specific α-ketoisocaproate. α-Ketoglutarate interferes only in that high concentrations decrease the color yield. This suitable detailed kinetic studies, even with crude tissue extracts, provided one corrects this effect of α-ketoglutarate. separate method was also described whereby between isoleucine (or valine) α-ketoglutarate can be measured, although not...
Pastes of white Portland cement (wPc) and wPc-pulverized fuel ash (pfa) blends were studied up to 13 years. The reaction wPc with water was initially retarded in the presence pfa particles but accelerated at intermediate ages. Reaction KOH solution rapid or without pfa. A universal compositional relationship exists for C-A-S-H Pc aluminosilicate-rich SCMs. average length aluminosilicate anions increased age increasing Al/Ca Si/Ca; greater lengthening due additional Al3 + bridging sites....
Abstract The obligatory methanotroph, Methylosinus trichosporium OB3b, was studied to optimize the batch culture conditions for formation of particulate methane monooxygenase (pMMO) in a nitrate minimal salts medium. important medium components investigated were copper, carbon dioxide, and nitrate. whole‐cell specific pMMO activity decreased sharply with increasing copper concentrations range 10–40 μ M remained constant upon further increases concentration 120 . cell growth rate (μ), on...
The aryl hydrocarbon receptor (AhR) mediates induction of CYP1A1 and CYP1B1 by 2,3,7,8-tetrachlorodibenzo-ρ-dioxin (dioxin) via binding to xenobiotic-responsive elements (XREs) in their enhancer regions. CYPIB1 were both inducible dioxin human MCF-7 cells. However, only was HepG2 Further experiments focused on providing an explanation for this last observation. Dioxin induced the recruitment AHR transcriptional coactivators p300 p300/cAMP response element-binding protein protein-associated...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTUse of Ligand Design to Provide Coordination Asymmetry in a Binuclear Metalloprotein Model System: Synthesis, Chemistry with Copper, and Demonstration Site-Directed ReactivityJoe H. Satcher Jr., Michael W. Droege, Timothy J. R. Weakley, Robert T. TaylorCite this: Inorg. Chem. 1995, 34, 12, 3317–3328Publication Date (Print):June 1, 1995Publication History Published online1 May 2002Published inissue 1 June...
Methanotrophs have promising applications in bioremediation and the production of fuel-related chemicals due to their nonspecific enzyme, methane monooxygenase (MMO). The optimal conditions for cell growth soluble from MMO (sMMO) were determined batch cultivations an obligatory methanotrophs, Methylosinus trichosporium OB3b, shake flasks a 5-L bioreactor. It was confirmed that copper deficiency is essential formation cytoplasmic sMNO. Optimum without added observed at pH 6.0-7.0, temperature...
Cytochrome P4502S1 (CYP2S1) is expressed at high levels in epithelial tissues and inducible by 2,3,7,8-tetrachlorodibenzo-p-dioxin (dioxin) via the aryl hydrocarbon receptor (AHR). Transcriptional initiation of mouse Cyp2s1 was found to occur three regions, approximately 198, 102, 22 nucleotides from translational codon. Approximately 400 upstream its codon, contains overlapping xenobiotic-responsive element (XRE) sequences, which make a major contribution toward dioxin inducibility. Each...
Although it is causative for many types of cancers, experimental and epidemiological evidence suggest that 2,3,7,8-tetrachlorodibenzo-p-dioxin (dioxin) may in fact protect against breast cancer. The mechanism(s) this protection remain unclear. In an attempt to further elucidate mechanism, we performed a microarray experiment identify genes were modulated upon dioxin treatment. We found downregulated the messenger RNAs G-protein-coupled receptor, CXCR4, as well its unique chemokine ligand,...
An auxotrophic mutant, GAT-, derived from the Chinese hamster cell line CHO-K1 and exhibiting multiple growth requirements for glycine, adenine, thymidine, has been shown to be deficient in one of folate-dependent enzymes, folylpolyglutamate synthetase (FPGS). This mutant was fused with normal human lymphocytes lymphoblasts 41 GAT + primary hybrid clones were isolated medium lacking thymidine. In addition, 71 secondary after four enriched medium, which allows losing complementing chromosome...
A simple method is described whereby millimicromole amounts of extensively purified N5-methyltetrahydrofolate-homocysteine transmethylase (vitamin B12 transmethylase) can be chemically propylated to yield an inhibited vitamin enzyme which reactivated with light. The procedure consists a short incubation the propyl iodide in presence reduced flavin mononucleotide and dithiothreitol under hydrogen gas. Formation (inhibited) was correlated alterations visible absorption spectrum enzyme. When...
The oceanographic environment surrounding the New England Seamounts frequently involves complex interaction of colder Slope Sea waters with warmer Sargasso waters, where a meandering frontal boundary is caused by Gulf Stream. sound speed profiles and associated available acoustic propagation paths differ significantly on either side Regional historical temperature profile data gathered from Argo float program are used to investigate common trends region. A clustering algorithm organizes...
A field test has demonstrated that an in situ biofilter using resting-state cells effectively remediated groundwater with about 425 ppb of trichloroethene (TCE) as the sole contaminant species. About 5.4 kg (dry weight equivalent) a strain methanotrophic bacteria (Methylosinus trichosporium OB3b) was suspended 1800 L (5.4 × 109 cells/mL) and injected into aquifer through single well at depth 27 m, several meters below water table. The devoid TCE growth substrates but amended phosphate...