Bacteriophages, the viruses infecting bacteria, are biological entities that can control their host populations. The ecological relevance of phages for microbial systems has been widely explored in aquatic environments, but current understanding role terrestrial ecosystems remains limited. Here, our objective was to quantify extent which drive assembly and functioning soil bacterial communities. We performed a reciprocal transplant experiment using natural sterilized incubated with different...

Abstract Soils are losing increasing amounts of carbon annually to freshwaters as dissolved organic matter (DOM), which, if degraded, can offset their sink capacity. However, the processes underlying DOM degradation across environments poorly understood. Here we show changes similarly along soil-aquatic gradients irrespective environmental differences. Using ultrahigh-resolution mass spectrometry, track soil depths and hillslope positions in forest catchments relate its composition...

Here we present MARVEL, a tool for prediction of double-stranded DNA bacteriophage sequences in metagenomic bins. MARVEL uses random forest machine learning approach. We trained the program on dataset with 1,247 phage and 1,029 bacterial genomes, tested it 335 177 genomes. show that three simple genomic features extracted from contig were sufficient to achieve good performance separating sequences: gene density, strand shifts, fraction significant hits viral protein database. compared...

This study focused on the effects of organic and inorganic amendments straw retention microbial biomass (MB) taxonomic groups bacteria in sugarcane-cultivated soils a greenhouse mesocosm experiment monitored for gas emissions chemical factors. The consisted combinations synthetic nitrogen (N), vinasse (V; liquid waste from ethanol production), sugarcane-straw blankets. Increases CO2-C N2O-N were identified shortly after addition both N V to soils, thus increasing MB (MB-N) decreasing carbon...

Abstract For the last 150 years many studies have shown importance of earthworms for plant growth, but exact mechanisms involved in process are still poorly understood. Many important functions required growth can be performed by soil microbes rhizosphere. To investigate earthworm influence on rhizosphere microbial community, we a macrocosm experiment with and without Pontoscolex corethrurus (EW+ EW−, respectively) followed various processes 217 days sugarcane. In EW+ treatments, N 2 O...

A presente pesquisa propõe uma reflexão crítica sobre a urgência de consolidar educação anticapacitista como fundamento ético, político e pedagógico para construção práticas educacionais verdadeiramente inclusivas. O estudo problematiza os limites das políticas inclusão que, embora avancem em termos legais, ainda reproduzem lógica integracionista, marcada pela tolerância normativa negação da diferença. Diante disso, indagamos: De que maneira perspectiva pode contribuir superação escolares...

Phages have a major impact on microbial populations. In this work, we discuss how predation, transduction, lysogeny, and phage domestication lead to symbio-centric genomic interactions between bacteria phage, ranging from antagonistic mutualistic. Furthermore, these influence bacterial diversification ecotype formation. We then propose an additional consideration in the form of ecological speciation framework for bacteria. Our builds upon classical morphological molecular taxonomy by also...

Composting is an important technique for environment-friendly degradation of organic material, and a microbe-driven process. Previous metagenomic studies composting have presented general description the taxonomic functional diversity its microbial populations, but they lacked more specific information on key organisms that are active during process.Here we present analyze 60 mostly high-quality metagenome-assembled genomes (MAGs) recovered from time-series samples two thermophilic cells,...

Global change is altering the vast amount of carbon cycled by microbes between land and freshwater, but how viruses mediate this process poorly understood. Here, we show that direct cycling in lake sediments, these impacts intensify with future changes water clarity terrestrial organic matter (tOM) inputs. Using experimental tOM gradients within sediments a clear dark boreal lake, identified 156 viral operational taxonomic units (vOTUs), which 21% strongly increased abundances key bacteria...

Abstract Permafrost thaw may increase the production of neurotoxic methylmercury (MeHg) in northern peatlands, but downstream delivery MeHg is uncertain. We quantified total mercury (THg) and concentrations lakes streams along a 1700 km permafrost transect boreal western Canada to determine influence regional extent compared local lake catchment characteristics. In lakes, we assessed sediment microbial communities modeled potential rates water column photodemethylation (PD). Regardless...

Northern lakes disproportionately influence the global carbon cycle, and may do so more in future depending on how their microbial communities respond to climate warming. Microbial can change because of direct effects warming metabolism indirect groundwater connectivity from thawing surrounding permafrost, especially at lower landscape positions. Here we used shotgun metagenomics compare taxonomic functional gene composition sediment microbes 19 peatland across a 1600-km permafrost transect...

Abstract Viruses are abundant and ubiquitous in soil, but their importance modulating greenhouse gas (GHG) emissions terrestrial ecosystems remains largely unknown. Here, various loads of viral communities introduced into paddy soils with different fertilization histories via a reciprocal transplant approach to study the role viruses regulating prokaryotic communities. The results showed that addition has strong impact on methane (CH 4 ) nitrous oxide (N 2 O) and, minor extent, carbon...

Abstract Small, organic‐rich lakes are important sources of methane (CH 4 ) and carbon dioxide (CO 2 to the atmosphere, yet sensitivity emissions climate warming is poorly constrained potentially influenced by permafrost thaw. Here, we monitored from 20 peatland across a 1,600 km transect in boreal western Canada. Contrary expectations, observed shift source sink CO for warmer regions, driven greater primary productivity associated with hydrological connectivity nutrient availability absence...

Every year around 300 Gl of vinasse, a by-product ethanol distillation in sugarcane mills, are flushed into more than 9 Mha cropland Brazil. This practice links fermentation waste management to fertilization for plant biomass production, and it is known as fertirrigation. Here we evaluate public datasets soil metagenomes mining changes antibiotic resistance genes (ARGs) soils from mesocosms repeatedly amended with vinasse. The were annotated using the ResFam database. We found that abundance...

Summary Viruses are now recognized as important players in microbial dynamics and biogeochemical cycles the oceans. Yet, compared with aquatic ecosystems, virus discovery terrestrial ecosystems has been challenging partly due to inherent complexity of soils. To expand our understanding soil viruses their putative contributions processes, we analysed metagenomes community‐level virus‐enriched suspensions by tangential flow filtration obtained from two French agricultural We found viral...

Abstract The rhizosphere is a vital soil compartment providing key plant‐beneficial functions. However, little known about the mechanisms driving viral diversity in rhizosphere. Viruses can establish lytic or lysogenic interactions with their bacterial hosts. In latter, they assume dormant state integrated host genome and be awakened by different perturbations that impact cell physiology, triggering bloom, which potentially fundamental mechanism diversity, as 22%–68% of bacteria are...

The rhizosphere microbiome plays a key role in plant protection against soil-borne pathogens. Plant breeding for resistance pathogens can alter the microbiome. However, most studies have focused on bacterial and fungal communities, leaving of virus viroids unassessed. Here, we tested influence composition viruses viroids. By analyzing metatranscriptomes from common bean (Phaseolus vulgaris) cultivars with varying to pathogen Fusarium oxysporum, recovered sequences representing 78 23 novel...

The microbial composition of the rhizosphere and greenhouse gas (GHG) emissions under most common input combinations in maize ( Zea mays L.) cultivated Brazil have not been characterized yet. In this study, we evaluated influence stover coverage (S), urea-topdressing fertilization (F), inoculant Azospirillum brasilense (I) on soil GHG communities during development. We conducted a experiment measured methane (CH 4 ), carbon dioxide (CO 2 nitrous oxide (N O) fluxes from with plants factorial...

Soils lose a large amount of carbon annually to freshwaters as dissolved organic matter (DOM), which, if degraded, can undermine climate change mitigation. The degradation state DOM in aquatic ecosystems reflect the distance from its source, with increasingly dominated by similar compounds proceeds. However, processes underlying and generality across environments are poorly understood. Here we found changed similarly along two soil-aquatic gradients irrespective environmental conditions. We...

Os autores externos submeteram sua publicação para apresentação de trabalho no evento "International Symposium on Immunobiologicals", que foi coordenado e organizado pelo Instituto Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz.

Os autores externos submeteram sua publicação para apresentação de trabalho no evento "International Symposium on Immunobiologicals", que foi coordenado e organizado pelo Instituto Tecnologia em Imunobiológicos (Bio-Manguinhos), da Fundação Oswaldo Cruz.

O projeto Out Of The Box emergiu como uma resposta à necessidade de oferecer um espaço reflexão e discernimento crítico no meio digital. Consiste em podcast, veiculado a plataformas digitais. Durante sua realização, foram realizados dez encontros, totalizando 3.321 visualizações, com média 332 visualizações por cada encontro. Observou-se boa adesão dos ouvintes, além promover reflexões sobre temas variados, contribuindo para que estes atinjam visão mais ampla contextos sociais própria realidade.