A5017601533- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- Magnetic properties of thin films
- Microfluidic and Bio-sensing Technologies
- Biosensors and Analytical Detection
- Quantum Dots Synthesis And Properties
- Characterization and Applications of Magnetic Nanoparticles
- Advanced MEMS and NEMS Technologies
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Complement system in diseases
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- ZnO doping and properties
- Mycotoxins in Agriculture and Food
- Immune Cell Function and Interaction
- Analytical Chemistry and Sensors
- Plasmonic and Surface Plasmon Research
- Block Copolymer Self-Assembly
- Physics of Superconductivity and Magnetism
- Acoustic Wave Resonator Technologies
MagArray (United States)
2008-2021
Cotton (United States)
2016-2021
Stanford University
2008-2011
Magnetic nanotags (MNTs) are a promising alternative to fluorescent labels in biomolecular detection assays, because minute quantities of MNTs can be detected with inexpensive giant magnetoresistive (GMR) sensors, such as spin valve (SV) sensors. However, translating this promise into easy use and multilplexed protein which highly sought after molecular diagnostics cancer diagnosis treatment monitoring, has been challenging. Here, we demonstrate multiplex potential markers at subpicomolar...
Synthetic antiferromagnetic (SAF) nanoparticles are fabricated by a combination of nanoimprint lithography and ferromagne tic multilayer structures, followed release stabilization in solution. The SAF have desirable magnetic properties with respect to multiplex labeling sorting, can be functionalized fluorescent dyes. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2008/adma200703077_s.pdf or from author. Please note: publisher...
Direct protein functionalization provides synthetic antiferromagnetic nanoparticles with high chemical specificity and multifunctionality. These nanoparticle-protein conjugates function as improved magnetic labels for biological detection experiments, exhibit tunable responses to a small external field gradient, thus allowing the observation of distinctive single nanoparticle motion.
Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood medical diagnosis. Using an vivo mouse radiation model, we developed protocols Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) small amounts of collected during the first week after X-ray exposures sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg showed excellent dose discrimination at ≥ 1 Gy time window to 7 days exposure except day 7. Saa1 response was limited two exposure. A multiplex assay...
Abstract Giant magnetoresistive (GMR) biosensors consisting of many rectangular stripes are being developed for high sensitivity medical diagnostics diseases at early stages, but aspects the sensing mechanism remain to be clarified. Using e-beam patterned masks on sensors, we showed that magnetic nanoparticles with a diameter 50 nm located between predominantly determine sensor signals over those stripes. Based computational analysis, it was confirmed particles in trench, particularly near...
Giant magnetoresistive (GMR) sensors are developed for a DNA microarray. Compared with the conventional fluorescent sensors, GMR cheaper, more sensitive, can generate fully electronic signals, and be easily integrated electronics microfluidics. The sensor used in this work has bottom spin valve structure an MR ratio of 12%. single-strand target detected length 20 bases. Assays concentrations down to 10 pM were performed, dynamic range 3 logs. A double modulation technique was signal...
We partition magnetic sensor arrays into individually addressable microfluidic compartments, enhancing their effective use,<italic>i.e.</italic>by separating cross-reactive assays.
Abstract Die direkte Proteinfunktionalisierung ergibt synthetische antiferromagnetische Nanopartikel mit hoher chemischer Spezifität und vielfältigen Funktionen. Diese Nanopartikel‐Protein‐Konjugate wirken als verbesserte magnetische Markierungen für biologische Analysen reagieren in einstellbarer Weise auf kleine äußere Magnetfeldgradienten, sodass sich die Bewegung einzelner verfolgen lässt. magnified image
We demonstrated that effects of serum matrix on molecular interactions between drugs and target proteins can be investigated in real time using magnetic bio-sensing techniques. A giant magneto-resistive (GMR) sensor was used which were fixed superparamagnetic nanoparticles (diameter: 50 nm) conjugated with drug phosphate buffer, without serum. In this study, the following drug-protein pairs investigated: quercetin cAMP-dependent protein kinase (PKA), Infliximab tumor necrosis factor alpha...
Giant magnetoresistive (GMR) biochips using magnetic nanoparticle as labels were developed for molecular diagnosis. The sensor arrays consist of GMR sensing strips 1.5 μm or 0.75 in width. sensors are exquisitely sensitive yet very delicate, requiring ultrathin corrosion-resistive passivation and efficient surface chemistry oligonucleotide probe immobilization. A mild stable was first that is especially suitable modifying delicate electronic device surfaces, a practical application our...
Abstract The ability to characterize the binding kinetics of drug-target interactions in a biologically relevant matrix, such as serum or plasma, remains fundamental challenge drug discovery. We apply novel label-based giant magnetoresistance (GMR) biosensor platform measure protein and affinities pairs buffer different levels serum. Specifically, we evaluate three well-established immune checkpoint inhibitors, pembrolizumab, nivolumab atezolizumab compare results with label-free kinetic...