Plant basic helix-loop-helix (bHLH) transcription factors play essential roles in abiotic stress tolerance. However, most bHLHs have not been functionally characterized. Here, we characterized the functional role of a bHLH factor from Arabidopsis, AtbHLH112, response to stress. AtbHLH112 is nuclear-localized protein, and its nuclear localization induced by salt, drought abscisic acid (ABA). In addition, serves as transcriptional activator, with activation domain located at N-terminus....

Syringe infiltration is an important transient transformation method that widely used in many molecular studies. Owing to the wide use of syringe agroinfiltration, it and necessary improve its efficiency. Here, we studied factors influencing efficiency agroinfiltration. The pCAMBIA1301 was transformed into Nicotiana benthamiana leaves for investigation. effects 5-azacytidine (AzaC), Ascorbate acid (ASC) Tween-20 on were studied. β-glucuronidase (GUS) expression GUS activity respectively...

Background: Glioma is the most common primary tumor of central nervous system. The conventional glioma treatment strategies include surgical excision and chemo- radiation-therapy. Interferon Gamma (IFN-γ) a soluble dimer cytokine involved in immune escape gliomas. In this study, we sought to identify IFN-γ-related genes construct prognostic model guide its clinical treatment. Methods: RNA sequences clinicopathological data were downloaded from Cancer Genome Atlas (TCGA) China (CGGA). Using...

Gliomas are the most frequent primary malignant brain tumors of central nervous system, causing significant impairment and death. There is mounting evidence that N7 methylguanosine (m7G) RNA dysmethylation plays a role in development progression cancer. However, expression patterns function m7G methylation regulator gliomas yet unknown. The goal this study was to examine 31 critical regulators linked with their prognostic significance gliomas. To begin, we systematically analyzed patient...

Abstract The homeodomain‐leucine zipper (HD‐Zip) proteins play crucial roles in plant developmental and environmental responses. However, how they mediate gene expression to facilitate abiotic stress tolerance remains unknown. In the present study, we characterized BpHOX2 (encoding a HD‐Zip I family protein) from birch ( Betula platyphylla ). is predominately expressed mature stems leaves, but at low level apical buds roots, suggesting that it has tissue‐specific characteristics. was highly...

Like other members of the family Reoviridae, rice black-streaked dwarf virus (RBSDV, genus Fijivirus) is thought to replicate and assemble within cytoplasmic viral inclusion bodies, commonly called viroplasms. RBSDV P9-1 key protein for formation viroplasms, but little known about proteins viroplasm or molecular interactions amongst its components. non-structural were screened their association with using a co-immunoprecipitation assay. Only P6 was found directly interact P9-1, an...

Abstract There has been an upward trend in the incidence of glioma, with high recurrence and mortality. The beta subunits 20S proteasome are encoded by (PSMB) genes may affect proteasome’s function assembly inhibitor binding. This study attempted to reveal proliferation invasion glioma cells, which is affected subunit 2 (PSMB2). We subjected data downloaded from TCGA database ROC, survival, enrichment analyses. After establishing stable PSMB2 knockdown cell line. detect changes...

Postoperative cerebral edema is common in patients with meningioma. It of great clinical significance to predict the postoperative exacerbation (CEE) for development individual treatment programs meningioma.To evaluate value three-dimensional radiomics Features from Multi-Parameter MRI predicting CEE meningioma.A total 136 meningioma complete and radiological data were collected this retrospective study, they randomly divided into primary validation cohorts. Three-dimensional features...

Glioblastoma is the most common primary malignant brain tumor. Because of limited understanding its pathogenesis, prognosis glioblastoma remains poor. This study was conducted to explore potential competing endogenous RNA (ceRNA) network chains and biomarkers in by performing integrated bioinformatics analysis.Transcriptome expression data from The Cancer Genome Atlas database Gene Expression Omnibus were analyzed identify differentially expressed genes between normal tissues. Biological...

Abstract DNA binding proteins carry out important and diverse functions in the cell, including gene regulation, but identifying these is technically challenging. In present study, we developed a technique to capture DNA-associated called reverse chromatin immunoprecipitation (R-ChIP). This technology uses set of specific probes labeled with biotin isolate chromatin, are then identified using mass spectrometry. Using R-ChIP, 439 that potentially bind promoter Arabidopsis thaliana AtCAT3...

INDETERMINATE-DOMAIN proteins (IDDs) are a plant-specific transcription factor family characterized by conserved ID domain with four zinc finger motifs. Previous studies have demonstrated that IDDs coordinate diversity of physiological processes and functions in plant growth development, including floral transition, architecture, seed root hormone signaling. In this review, we especially summarized the latest knowledge on working models IDD members Arabidopsis, rice, maize, particularly...

Background: Glioma is the most common primary tumor of central nervous system and associated with poor overall survival, creating an urgent need to identify survival-associated biomarkers. C1ORF112, alpha-helical protein, overexpressed in some cancers; however, its prognostic role has not yet been explored gliomas. Thus, this study, we attempted address by determining value potential function C1ORF112 low-grade gliomas (LGGs). Methods: The expression normal tissues was analyzed using data...

As a member of integrin receptor family, ITGAV (integrin subunit α V) is involved in variety cell biological processes and overexpressed various cancers, which may be potential prognostic factor. However, its value function lower-grade glioma (LGG) are still unclear, terms immune infiltration, it has not been fully elucidated. Here, the expression preference, value, clinical traits were investigated using The Cancer Genome Atlas database (n = 528) Chinese Glioma dataset 458). Gene Ontology...

Abstract There has been an upward trend in the incidence of glioma, with high recurrence and mortality. The beta subunits 20S proteasome are encoded by (PSMB) genes may affect proteasome's function assembly inhibitor binding. This study attempted to reveal proliferation invasion glioma cells, which is affectedby subunit 2 (PSMB2). We subjected data downloaded from TCGA database ROC, survival, enrichment analyses. PSMB2 expression was verified quantitative PCR Western blotting identify...

This study analyzed sequencing and clinical data from the Cancer Genome Atlas (TCGA) gene expression synthesis, used Chinese glioma (CGGA) for external validation. The of DCP2 in normal brain tumor tissue was compared. We molecular characteristics prognostic value glioma. In addition, levels were evaluated 30 samples upregulated compared to (

Abstract Background Glioblastoma is the most common primary malignant brain tumor. Due to limited understanding of its pathogenesis, prognosis glioblastoma poor. The purpose this study explore potential ceRNA network chains and biomarkers in through integrated bioinformatics analysis. Methods Transcriptome expression data from Cancer Genome Atlas database Gene Expression Omnibus were analyzed identify differentially expressed genes between tissue normal tissue. biological pathways associated...

Abstract Background: Glioblastoma is the most common primary malignant brain tumor. Because of limited understanding its pathogenesis, prognosis glioblastoma remains poor. This study was conducted to explore potential competing endogenous RNA (ceRNA) network chains and biomarkers in by performing integrated bioinformatics analysis. Methods: Transcriptome expression data from The Cancer Genome Atlas database Gene Expression Omnibus were analyzed identify differentially expressed genes between...

Abstract There has been an upward trend in the incidence of glioma, with high recurrence and mortality. The beta subunits 20S proteasome are encoded by (PSMB) genes may affect proteasome's function assembly inhibitor binding. This study attempted to reveal proliferation invasion glioma cells, which is affected subunit 2 (PSMB2). We subjected data downloaded from TCGA database ROC, survival, enrichment analyses. PSMB2 expression was verified quantitative PCR Western blotting identify mRNA...

Functional magnetic resonance imaging (fMRI) has been used for evaluating residual brain function and predicting the prognosis of patients with severe traumatic injury (sTBI). This study aimed to integrate fractional amplitude low-frequency fluctuation (fALFF) functional connectivity (FC) investigate mechanism sTBI.Sixty-five sTBI were included underwent fMRI scanning within 14 days after injury. The patient's outcome was assessed using Glasgow Outcome Scale-Extended (GOSE) at 6 months...