A5055369091- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Asymmetric Hydrogenation and Catalysis
- Plant Parasitism and Resistance
- Synthesis and Catalytic Reactions
- Plant and animal studies
- Catalytic C–H Functionalization Methods
- Pharmacogenetics and Drug Metabolism
- Allelopathy and phytotoxic interactions
- Chemical Reactions and Isotopes
- Plant Stress Responses and Tolerance
- Enzyme Catalysis and Immobilization
- Biochemical and Molecular Research
- Plant Molecular Biology Research
- Mass Spectrometry Techniques and Applications
- Chemical Synthesis and Analysis
- Lysosomal Storage Disorders Research
- Escherichia coli research studies
University of California, Davis
2023-2024
University of York
2020-2023
The development of efficient and sustainable methods for the synthesis nitrogen heterocycles is an important goal chemical industry. In particular, substituted chiral piperidines are prominent targets due to their prevalence in medicinally relevant compounds precursors. A potential biocatalytic approach this privileged scaffold would be asymmetric dearomatization readily assembled activated pyridines. However, nature yet yield a suitable biocatalyst specifically reaction. Here, by combining...
Imine reductases (IREDs) catalyze the asymmetric reduction of cyclic imines, but also in some cases coupling ketones and amines to form secondary amine products an enzyme-catalyzed reductive amination (RedAm) reaction. Enzymatic RedAm reactions have typically used small hydrophobic amines, many interesting pharmaceutical targets require that larger be these reactions. Following identification IR77 from Ensifer adhaerens as a promising biocatalyst for cyclohexanone with pyrrolidine, we...
Abstract Phytohormone levels are regulated through specialized enzymes, participating not only in their biosynthesis but also post-signaling processes for signal inactivation and cue depletion. Arabidopsis thaliana (At) carboxylesterase 15 (CXE15) 20 (CXE20) have been shown to deplete strigolactones (SLs) that coordinate various growth developmental function as signaling molecules the rhizosphere. Here, we elucidate X-ray crystal structures of AtCXE15 (both apo SL intermediate bound)...
α-Mannoside β-1,6-N-acetylglucosaminyltransferase V (MGAT5) is a mammalian glycosyltransferase involved in complex N-glycan formation, which strongly drives cancer when overexpressed. Despite intense interest, the catalytic mechanism of MGAT5 not known detail, precluding therapeutic exploitation. We solved structures complexed to glycosyl donor and acceptor ligands, revealing an unforeseen role for donor-induced loop rearrangements controlling substrate engagement. QM/MM metadynamics...
Biocatalytic reductive amination reactions with aminases (RedAms) are emerging transformations a high potential value for pharmaceutical synthesis. Here, we report the identification and engineering of RedAm to catalyze reaction, making key intermediate in synthesis an investigational cyclin-dependent kinase (CDK) inhibitor, using relatively bulky benzylamine as nucleophile. The engineered enzyme contains six mutations respect wild-type displays productivity at substrate concentrations...
Abstract KAI2 proteins, classified as plant α/β hydrolase receptors, are capable of perceiving smoke- derived butenolide signals and endogenous, yet unidentified KAI2-ligands (KLs). While the number functional receptors varies among land species, rice possesses only one gene. Rice, a significant crop representative grasses, relies on KAI2-mediated Arbuscular mycorrhiza (AM) symbioses to flourish in traditionally arid nutrient-poor environments. This study presents first crystal structure an...
The development of efficient and sustainable methods for the synthesis nitrogen heterocycles is an important goal chemical industry. In particular, substituted chiral piperidines are prominent targets due to their prevalence in medicinally relevant compounds precursors. A potential biocatalytic approach this privileged scaffold would be asymmetric dearomatization readily assembled activated pyridines. However, nature has yet yield a suitable biocatalyst specifically reaction. Here, by...
The development of efficient and sustainable methods for the synthesis nitrogen heterocycles is an important goal chemical industry. In particular, substituted chiral piperidines are prominent targets due to their prevalence in medicinally relevant compounds precursors. A potential biocatalytic approach this privileged scaffold would be asymmetric dearomatization readily assembled activated pyridines. However, nature has yet yield a suitable biocatalyst specifically reaction. Here, by...