A5032244305- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Microbial Community Ecology and Physiology
- Enzyme Production and Characterization
- Ubiquitin and proteasome pathways
- Cancer therapeutics and mechanisms
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Microbial Natural Products and Biosynthesis
- Algal biology and biofuel production
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- DNA and Nucleic Acid Chemistry
- Microbial bioremediation and biosurfactants
- Plant Pathogens and Fungal Diseases
Thermo Fisher Scientific (Germany)
2020
Ludwig-Maximilians-Universität München
2007
Center for Integrated Protein Science Munich
2007
Philipps University of Marburg
2006
DNA polymerase η (Pol η) is a eukaryotic lesion bypass that helps organisms to survive exposure ultraviolet (UV) radiation, and tumor cells gain resistance against cisplatin-based chemotherapy. It allows replicate across cross-link lesions such as 1,2-d(GpG) cisplatin adducts (Pt-GG) UV-induced cis – syn thymine dimers. We present structural biochemical analysis of how Pol copies Pt-GG–containing DNA. The damaged bound in an open binding rim. Nucleotidyl transfer requires the rotate into...
Actinomycetes play a relevant role in soil ecology and are also of important biotechnological interest as they produce several bioactive metabolites. Within the filamentous actinomycetes, it would be desirable to recognize characterize environmental samples containing unusual genera. To this end, we have developed selective primer sets for polymerase chain reaction (PCR) amplification 16S rDNA from Actinomycetales families Micromonosporaceae, Streptomycetaceae, Streptosporangiaceae...
Isolation of high molecular weight DNA fragments from soil, in excess 1 Mb, and sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination indirect extraction cells, using a nycodenz technique, followed by lysis biomass immobilised agarose plugs, allowed Mb to be purified.
De novo gene synthesis is the state-of-the-art method used to obtain genetic material adapted requirements of host organism and a cornerstone for modern synthetic biology. Yet, little progress has been made regarding downstream processes protein production from material. The recombinant proteins traditionally requires extensive preparatory work including amplification, cloning, sequencing, transformation or transfection expression host, cultivation living cells, purification overexpressed...