A5107015663- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Nanopore and Nanochannel Transport Studies
- Cancer Genomics and Diagnostics
University of Bath
2024-2025
5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are modified versions of cytosine in DNA with roles regulating gene expression. Using whole genomic from mouse cerebellum, we benchmark 5mC 5hmC detection by Oxford Nanopore Technologies sequencing against other standard techniques. In addition, assess the ability duplex base-calling to study strand asymmetric modification. is accurate relative compared techniques opens means studying these modifications. Strand modification...
Background: Colorectal cancer (CRC) progression from adenoma to adenocarcinoma is associated with global reduction in 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). DNA hypomethylation continues upon liver metastasis. Here we examine 5hmC changes Results: increased metastatic tissue relative the primary colon tumour expression of TET2 TET3 negatively correlated risk for metastasis patients CRC. Genes show KEGG enrichment adherens junctions, cytoskeleton cell migration around a...
Abstract Background Colorectal cancer (CRC) progression from adenoma to adenocarcinoma is associated with global reduction in 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). DNA hypomethylation continues upon liver metastasis. Here we examine 5hmC changes Results increased metastatic tissue relative the primary colon tumour expression of TET2 TET3 negatively correlated risk for metastasis patients CRC. Genes show KEGG enrichment adherens junctions, cytoskeleton cell migration...
<title>Abstract</title> 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are modified versions of cytosine in DNA with roles regulating gene expression. Using whole genomic from mouse cerebellum, we have benchmarked 5mC 5hmC detection by Oxford Nanopore Technologies sequencing against other standard techniques. In addition, assessed the ability duplex base-calling to study strand asymmetric modification. is accurate relative compared techniques opens new means studying these...