A5054379235- Fungal and yeast genetics research
- Fermentation and Sensory Analysis
- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Microbial Metabolic Engineering and Bioproduction
- Pesticide and Herbicide Environmental Studies
- Yeasts and Rust Fungi Studies
- Horticultural and Viticultural Research
- Vibrio bacteria research studies
- Biofuel production and bioconversion
- RNA and protein synthesis mechanisms
- Identification and Quantification in Food
- Fungal Plant Pathogen Control
- Essential Oils and Antimicrobial Activity
- Nematode management and characterization studies
- Forest Ecology and Biodiversity Studies
- Sulfur Compounds in Biology
- Plant Pathogens and Fungal Diseases
- Antibiotics Pharmacokinetics and Efficacy
- Genetically Modified Organisms Research
- Bacterial Identification and Susceptibility Testing
- Infections and bacterial resistance
- Wine Industry and Tourism
University of Florence
2008-2022
University of Parma
2007
University of Chieti-Pescara
1988-2002
The present study aimed to evaluate the impact of polycyclic aromatic hydrocarbons (PAHs) produced by multiple emission sources on prokaryotic communities in sediments chronically affected anthropogenic pressures. In this context, surface were investigated three Mediterranean touristic ports over sampling periods and different port sectors. levels 16 priority PAHs varied orders magnitude (25-49,000 ng g-1) covering range concentrations previously reported for harbors. Pyrogenic processes...
The structural gene of the Proteus mirabilis glutathione transferase GSTB1-1 (gstB) has been isolated from genomic DNA. A nucleotide sequence determination gstB predicted a translational product 203 amino acid residues, perfectly matching previously purified protein [Mignogna, Allocati, Aceto, Piccolomini, Di Ilio, Barra and Martini (1993) Eur. J. Biochem. 211, 421–425]. P. GST revealed 56% identity with Escherichia coli at DNA level 54% identity. Similarity also translation products...
Ports and marinas are central nodes in transport network play a strategic role coastal development. They receive pollution from land-based sources, marine traffic port infrastructures on one side constitute potential source for the adjacent areas other. The aim of present study was to evaluate effects organic inorganic co-contamination prokaryotic communities sediments three Mediterranean ports. structure composition bacterial archaeal were assessed by targeted metagenomic analysis 16S rRNA...
Five glutathione transferase (GST) forms were purified from human uterus by glutathione-affinity chromatography followed chromatofocusing, and their structural, kinetic immunological properties investigated. Upon SDS/polyacrylamide slab gel electrophoresis all resulted composed of two subunits identical molecular size. GST V (pI 4.5) is a dimer 23-kDa subunits. I 6.8) IV 4.9) are dimers 24-kDa whereas II 6.1) III 5.5) 26.5-kDa accounts for about 85-90% the activity other isoenzymes present...
The role of the evolutionarily conserved residue Pro-53 in Proteus mirabilis glutathione transferase B1-1 has been examined by replacing it with a serine using site-directed mutagenesis. effect replacement on activity, thermal stability and antibiotic binding capacity enzyme was examined. results presented support view that participates maintenance proper conformation fold rather than playing direct catalytic reaction. Furthermore, this appears to be an important determinant enzyme....
In order to investigate the roles of near N‐terminus Tyr, Cys, and Ser residues in activity bacterial glutathione transferase (GSTB1‐1) site‐directed mutagenesis was used replace following residues: Tyr‐4, Tyr‐5, Ser‐9, Cys‐10, Ser‐11, Ser‐13. The results presented here show that, unlike all other alpha, mu, pi, theta sigma classes transferases so far investigated, GSTB1‐1 does not utilise any or Cys residue activate glutathione. These also suggest that may require classification into their...
The strain diversity and the population structure of nosocomial Acinetobacter isolated from patients admitted to different hospitals in Florence, Italy, during a 3-year surveillance program, were investigated by amplified fragment length polymorphism (AFLP). majority isolates (84.5%) identified as A. baumannii, confirming this species most common hospital Acinetobacter. Three very distinct baumannii clonal groups (A1, A2, A3) defined. A1 appeared be genetically related well-characterized...
Summary Seiridium cardinale is regarded as the most important agent responsible for disease of cupressaceous hosts referred to Cypress canker. The fungus was first described in C alifornia and currently reported all continents. A recent study based on seven SSR loci has suggested that populations may represent source epidemic M editerranean. In this study, 185 AFLP markers were used an expanded sample size 125 isolates determine whether editerranean population indeed be derived from one...
Cefixime (CEF) is a cephalosporin included in the WHO Model List of Essential Medicines for Children. Liquid formulations are considered best choice pediatric use, due to their great ease administration and dose-adaptability. Owing its very low aqueous solubility poor stability, CEF only available as powder oral suspensions, which can lead reduced compliance by children, unpleasant texture taste, possible non-homogeneous dosage. The aim this work was develop an solution endowed with good...
The function of the open reading frame (ORF) YOR108w Saccharomyces cerevisiae has been analysed. deletion this ORF from chromosome XV did not give an identifiable phenotype. A mutant in which both and LEU4 gene have deleted proved to be leucine auxotrophic α-isopropylmalate synthase (α-IPMS)-negative. This recovered α-IPMS activity a Leu+ phenotype when transformed with plasmid copy YOR108w. These data sequence homology indicated that is structural for II, responsible residual found leu4Δ...
Mutagenesis induced in the yeast Saccharomyces cerevisiae by starvation for nutrilites is a well-documented phenomenon of an unknown mechanism. We have previously shown that polymerase delta proofreading activity controls spontaneous mutagenesis cells starved histidine. To obtain further information, we compared effect adenine on wild-type and, lacking (phenotype Exo-, mutation pol3-01).Ade+ revertants accumulated at very high rate adenine-free plates so their frequency day 16 after plating...