A5059349793- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Helicobacter pylori-related gastroenterology studies
- Epigenetics and DNA Methylation
- Antibiotics Pharmacokinetics and Efficacy
- Urinary Tract Infections Management
- Porphyrin Metabolism and Disorders
- Sulfur Compounds in Biology
- Antibiotic Resistance in Bacteria
- Selenium in Biological Systems
- Redox biology and oxidative stress
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Veterinary medicine and infectious diseases
- Antimicrobial Resistance in Staphylococcus
- Gastroesophageal reflux and treatments
- Probiotics and Fermented Foods
- Drug Transport and Resistance Mechanisms
- Galectins and Cancer Biology
- Bat Biology and Ecology Studies
- Folate and B Vitamins Research
- Metabolism and Genetic Disorders
- Enterobacteriaceae and Cronobacter Research
- Zoonotic diseases and public health
- Research on Leishmaniasis Studies
University of Chieti-Pescara
2012-2025
Istituti di Ricovero e Cura a Carattere Scientifico
1996-2002
University of Rome Tor Vergata
2002
University of L'Aquila
2002
St Vincents Institute of Medical Research
2002
Istituto di Farmacologia Traslazionale
1991
The antibacterial effect of aqueous garlic extract (AGE) was investigated against Helicobacter pylori. Sixteen clinical isolates and three reference strains H. pylori were studied. Two different varieties used. concentration AGE required to inhibit the bacterial growth between 2–5 mg ml−1. concentration, for both types, 90% (MIC90) 5 minimum bactericidal (MBC) usually equal to, or two-fold higher than, inhibitory (MIC). Heat treatment extracts reduced activity pylori; boiled showed a loss...
Abstract An experimental rodent model was used to demonstrate the viability of coccoid form Helicobacter pylori . Concentrated suspensions were prepared for two different morphologies: at 2 days incubation bacillary forms and 20 “dormant” forms. The strains fresh isolates from humans with duodenal ulceration, collection strains. Five hundred microliters culture (OD 550 = 5 Mc Farland) (2‐5×10 9 CFU/ml) (0 morphology inoculated intragastrically in BALB/c mice. gastric mucosa mice colonized by...
Three forms of glutathione transferase (GST) with pI values 6.0, 6.4 and 7.3 were isolated from Proteus mirabilis AF 2924 by glutathione-affinity chromatography followed isoelectric focusing, their structural, kinetic immunological properties investigated. Upon SDS/polyacrylamide-slab-gel electrophoresis, all proved to be composed two subunits identical (22,500) Mr. GST-6.0 GST-6.4 together account for about 95% the total activity, whereas GST-7.3 is present only in trace amounts. Extensive...
Abstract The morphologic changes from bacillary to coccoid forms of Helicobacter pylori were studied. These form analyzed by bacterial growth in Brucella broth plus 2% fetal calf serum. observed at five days incubation and a rapid decrease CFU/ml was recorded. At two weeks microaerophilic incubation, all not culturable vitro. morphology earlier when the culture H. incubated aerobic conditions with subinhibitory concentrations omeprazole roxithromycin. To evaluate possibility resistance...
Journal Article Expression of glutathione S-transferase and peptide methionine sulphoxide reductase in Ochrobactrum anthropi is correlated to the production reactive oxygen species caused by aromatic substrates Get access Antonio Tamburro, Tamburro Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Negri Sud, "Gennaro Paone", Environmental Health Center, Via Nazionale, 66030 Santa Maria Imbaro, Italy Search for other works this author on: Oxford Academic PubMed Google Scholar Iole...
The presence of glutathione transferase (GST; EC 2.5.1.18) in Escherichia coli ATCC 25922, E. 25422, Proteus vulgaris 8427, Pseudomonas aeruginosa 27853, Klebsiella oxytoca CIP 666, K. AF 101, Enterobacter cloacae 6085, Serratia marcescens 6755, and mirabilis 2924 was investigated. Using 1-chloro-2,4-dinitrobenzene as substrate, GST activity found the glutathione-(GSH-)affinity-purified fraction all strains tested. SDS-PAGE analysis GSH-affinity-purified enzyme indicated that GSTs these...
Glutathione transferases (GSTs) are protection enzymes capable of conjugating glutathione (GSH) to toxic compounds. During evolution an important catalytic cysteine residue involved in GSH activation was replaced by serine or, more recently, tyrosine. The utility these replacements represents enigma because they yield no improvements the affinity toward or its reactivity. Here we show that changes better protect cell from nitric oxide (NO) insults. In fact dinitrosyl·diglutathionyl·iron...
The structural gene of the Proteus mirabilis glutathione transferase GSTB1-1 (gstB) has been isolated from genomic DNA. A nucleotide sequence determination gstB predicted a translational product 203 amino acid residues, perfectly matching previously purified protein [Mignogna, Allocati, Aceto, Piccolomini, Di Ilio, Barra and Martini (1993) Eur. J. Biochem. 211, 421–425]. P. GST revealed 56% identity with Escherichia coli at DNA level 54% identity. Similarity also translation products...
We have identified an N-capping box motif (Ser/Thr-Xaa-Xaa-Asp) that is strictly conserved, at the beginning of α6 helix, in all glutathione S-transferases (GSTs) and most related superfamily proteins. By using CD peptide modelling we demonstrated capping residues important role determining helical conformation adopted by this fragment hydrophobic environment protein. This example which a local motif, contributing to nucleation structural element essential global folding protein, conserved homologous
We investigated the effects of several xenobiotics, including antimicrobial agents and general stress factors such as starvation, heat osmotic shock, on modulation expression Proteus mirabilis glutathione S-transferase B1-1 (PmGST B1-1). The level PmGST was established by both Western- Northern-blot experiments. Our results show that compounds can modulate B1-1. increased when bacterial cells were exposed to a variety stresses 1-chloro-2,4-dinitrobenzene, H(2)O(2), fosfomycin or...
The native form of the bacterial glutathione transferase B1-1 (EC ) is characterized by one (GSH) molecule covalently linked to Cys-10. This peculiar disulfide, only found in Beta and Omega class S-transferases (GSTs) but absent all other GSTs, prompts questions about its role how GSH can be activated utilized reaction normally performed GSTs. Stopped-flow spectroscopic experiments suggest that, enzyme (GSTB1-1ox), a second present, albeit transiently, active site. binds through bimolecular...
Coronavirus disease 2019 (COVID-19) is characterized by a broad spectrum of clinical symptoms. After acute infection, some subjects develop post-COVID-19 syndrome known as long-COVID. This study aims to recognize the molecular and functional mechanisms that occur in COVID-19 long-COVID patients identify useful biomarkers for management with Here, we profiled response performing proteomic analysis lymphocytes isolated from patients. We identified significant changes proteins involved iron...
The role of the evolutionarily conserved residue Pro-53 in Proteus mirabilis glutathione transferase B1-1 has been examined by replacing it with a serine using site-directed mutagenesis. effect replacement on activity, thermal stability and antibiotic binding capacity enzyme was examined. results presented support view that participates maintenance proper conformation fold rather than playing direct catalytic reaction. Furthermore, this appears to be an important determinant enzyme....