A5063096028- Bacterial Genetics and Biotechnology
- Botulinum Toxin and Related Neurological Disorders
- Genomics and Phylogenetic Studies
- Neurological disorders and treatments
- Cancer Treatment and Pharmacology
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Biochemical and Structural Characterization
- Hereditary Neurological Disorders
- RNA and protein synthesis mechanisms
Ipsen (United Kingdom)
2016-2020
University of Nottingham
2013-2018
In bacteria, the highly conserved RsmA/CsrA family of RNA-binding proteins functions as global posttranscriptional regulators acting on mRNA translation and stability. Through phenotypic complementation an rsmA mutant in Pseudomonas aeruginosa, we discovered a member, termed RsmN. Elucidation RsmN crystal structure that complex with hairpin from sRNA, RsmZ, reveals uniquely inserted α helix, which redirects polypeptide chain to form distinctly different protein fold domain-swapped dimeric...
Pseudomonads typically carry multiple non-identical alleles of the post-transcriptional regulator rsmA. In Pseudomonas aeruginosa, RsmN is notable in that its structural rearrangement confers distinct and overlapping functions with RsmA. However, little known about specificities for target RNAs overall impact on biology this pathogen. We purified mapped 503 transcripts directly bound by P. aeruginosa. About 200 mRNAs identified encode proteins demonstrated function including some determining...
Botulinum neurotoxins (BoNTs) are a family of bacterial toxins with seven major serotypes (BoNT/A–G). The ability these to target and bind motor nerve terminals is key factor determining their potency efficacy. Among toxins, BoNT/B one the two types approved for medical cosmetic uses. Besides binding well-established receptors, an extended loop in C-terminal receptor-binding domain (HC) (HC/B) has been proposed also contribute toxin neurons by interacting lipid membranes (termed...