A5092097374- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Photochromic and Fluorescence Chemistry
- PARP inhibition in cancer therapy
- Integrated Circuits and Semiconductor Failure Analysis
- Advanced Fluorescence Microscopy Techniques
- Bacterial Genetics and Biotechnology
- Nanopore and Nanochannel Transport Studies
Peter the Great St. Petersburg Polytechnic University
2022-2024
Science for Life Laboratory
2024
Uppsala University
2024
ORCID
2022
Abstract The chromatin remodeler ALC1 is activated by DNA damage-induced poly(ADP-ribose) deposited PARP1/PARP2 and their co-factor HPF1. has emerged as a cancer drug target, but how it recruited to ADP-ribosylated nucleosomes affect positioning near breaks unknown. Here we find that PARP1/HPF1 preferentially initiates ADP-ribosylation on the histone H2B tail closest break. To dissect consequences of such asymmetry, generate with defined one side only. cryo-electron microscopy structure...
Abstract Many essential processes in the cell depend on proteins that use nucleoside triphosphates (NTPs). Methods directly monitor often-complex dynamics of these at single-molecule level have helped to uncover their mechanisms action. However, measurement throughput is typically limited for NTP-utilizing reactions, and quantitative dissection complex over multiple sequential turnovers remains challenging. Here we present a method controlling NTP-driven reactions experiments via local...
Single-molecule techniques are ideally poised to characterize complex dynamics but typically limited investigating a small number of different samples. However, large sequence or chemical space often needs be explored derive comprehensive understanding biological processes. Here we describe multiplexed single-molecule characterization at the library scale (MUSCLE), method that combines fluorescence microscopy with next-generation sequencing enable highly observations dynamics. We...
Antibiotic streptolydigin (Stl) inhibits bacterial transcription by blocking the trigger loop folding in active center of RNA polymerase (RNAP), which is essential for catalysis. We use acoustic force spectroscopy to characterize dynamics elongation ternary complexes (ECs) RNAP presence Stl at a single-molecule level. found that induces long-lived stochastic pauses while instantaneous velocity between unaffected. enhances short-lived associated with an off-pathway early paused state...
Antibiotic streptolydigin (Stl) inhibits bacterial transcription by blocking the trigger loop folding in active center of RNA polymerase (RNAP), which is essential for catalysis. We use acoustic force spectroscopy to characterize dynamics elongation ternary complexes RNAP (ECs) presence Stl at a single-molecule level. found that induces long-lived stochastic pauses while instantaneous velocity between unaffected. enhances short-lived associated with an off-pathway elemental paused state...