A5091776919- Microbial Community Ecology and Physiology
- Marine Biology and Ecology Research
- Bacteriophages and microbial interactions
- Marine and coastal ecosystems
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Marine and fisheries research
- Environmental DNA in Biodiversity Studies
- Plant Virus Research Studies
- Crustacean biology and ecology
- Coral and Marine Ecosystems Studies
- Vibrio bacteria research studies
- Fish biology, ecology, and behavior
- Respiratory viral infections research
- Marine and coastal plant biology
Technion – Israel Institute of Technology
2018-2025
California State University, Long Beach
2025
University of Washington
2016-2020
Brigham Young University
2010
Abstract The photosynthetic picocyanobacteria Prochlorococcus and Synechococcus are models for dissecting how ecological niches defined by environmental conditions, but interactions with bacteriophages affect picocyanobacterial biogeography in open ocean biomes has rarely been assessed. We applied single-virus single-cell infection approaches to quantify cyanophage abundance infected 87 surface water samples from five transects that traversed approximately 2,200 km the North Pacific Ocean on...
Abstract Siderophores are strong iron‐binding molecules produced and utilized by microbes to acquire the limiting nutrient iron (Fe) from their surroundings. Despite importance as a component of ligand pool in seawater, data on distribution siderophores that use them limited. Here, we measured concentrations types dissolved during two cruises April 2016 June 2017 transited iron‐replete, low‐macronutrient North Pacific Subtropical Gyre through Transition Zone (NPTZ) iron‐deplete,...
Abstract Aim The sequential break‐up of Gondwana is thought to be a dominant process in the establishment shared biota across landmasses Southern Hemisphere. Yet similar distributions are by taxa whose radiations clearly post‐date Gondwanan break‐up. Thus, determining contribution vicariance versus dispersal seemingly complex. southern freshwater crayfishes (family Parastacidae) distributed on Australia and New Guinea, South America, Madagascar Zealand unlikely have dispersed via oceans,...
Up to half of marine N losses occur in oxygen-deficient zones (ODZs). Organic matter flux from productive surface waters is considered a primary control on N2 production. Here we investigate the offshore Eastern Tropical North Pacific (ETNP) where secondary chlorophyll maximum resides within ODZ. Rates production and carbon export mixed layer productivity were consistent with oligotrophic waters. However, sediment trap nitrogen fluxes increased between 105 150 m, indicating organic...
Abstract Long-term stability of picocyanobacteria in the open oceans is maintained by a balance between synchronous division and death on daily timescales. Viruses are considered major source microbial mortality, however, current methods to measure infection have significant methodological limitations. Here we describe method that pairs flow-cytometric sorting with PCR-based polony technique simultaneously screen thousands taxonomically resolved individual cells for intracellular virus DNA,...
Photosynthesis fuels primary production at the base of marine food webs. Yet, in many surface ocean ecosystems, diel-driven is tightly coupled to daily loss. This tight coupling raises question: which top-down drivers predominate maintaining persistently stable picocyanobacterial populations over longer time scales? Motivated by high-frequency water measurements taken North Pacific Subtropical Gyre (NPSG), we developed multitrophic models investigate bottom-up and mechanisms underlying...
SeaFlow is an underway flow cytometer that provides continuous shipboard observations of the abundance and optical properties small phytoplankton (<5 μm in equivalent spherical diameter, ESD). Here we present data sets consisting SeaFlow-based cell abundance, forward light scatter, pigment fluorescence individual cells, as well derived estimates ESD cellular carbon content picophytoplankton, which includes cyanobacteria Prochlorococcus, Synechococcus small-sized Crocosphaera ESD),...
Abstract Marine cyanobacteria of the genera Synechococcus and Prochlorococcus are most abundant photosynthetic organisms on earth, spanning vast regions oceans contributing significantly to global primary production. Their viruses (cyanophages) greatly influence cyanobacterial ecology evolution. Although many cyanophage genomes have been sequenced, insight into functional role genes is limited by lack a genetic engineering system. Here, we describe simple, generalizable method for...
ABSTRACT Cyanobacterial distributions are shaped by abiotic factors including temperature, light and nutrient availability as well biotic such grazing viral infection. In this study, we investigated the abundances of T4‐like T7‐like cyanophages extent picocyanobacterial infection in cold, high‐nutrient‐low‐chlorophyll, sub‐Antarctic waters southwest Pacific Ocean during austral spring. Synechococcus was dominant picocyanobacterium, ranging from 4.7 × 10 3 to 1.2 5 cells∙mL −1 , while...
Cyanophages encode host-derived genes that may increase their fitness. We examined the relative abundance of 18 cyanophages in metagenomes and viromes along depth profiles from Eastern Tropical North Pacific Oxygen Deficient Zone (ETNP ODZ) where Prochlorococcus dominates a secondary chlorophyll maximum within ODZ. at oxic primary encoded related to light phosphate stress (psbA, psbD pstS T4-like psbA T7-like), but proportion cyanophage with these decreased depth. The purine biosynthesis...
Abstract Siderophores are strong iron-binding molecules produced and utilized by microbes to acquire the limiting nutrient iron (Fe) from their surroundings. Despite importance as a component of ligand pool in seawater, data on distribution siderophores that use them limited. Here we measured concentrations types dissolved during two cruises April 2016 June 2017 transited iron-replete, low-macronutrient North Pacific Subtropical Gyre (NPSG) through Transition Zone (NPTZ) iron-deplete,...
Environmental virus communities are highly diverse. However, the infection physiology underlying evolution of diverse phage lineages and their ecological consequences largely unknown. T7-like cyanophages abundant in nature infect marine unicellular cyanobacteria, Synechococcus Prochlorococcus, important primary producers oceans. Viruses belonging to this genus divided into two distinct phylogenetic clades: clade A B. These viruses have narrow host-ranges with phages primarily infecting...
Viruses are catalysts of biogeochemical cycling, architects microbial community structure, and terminators phytoplankton blooms. Viral lysis diatoms, a key group eukaryotic phytoplankton, has the potential to impact carbon export marine food webs. However, viruses on diatom abundance composition is unknown. Diatom-virus dynamics were explored by sampling every month at two coastal estuarine locations in Washington state, USA resulting 41 new isolates pennate Pseudo-nitzschia 20 environmental...
OPINION article Front. Microbiol., 01 March 2018Sec. Aquatic Microbiology Volume 9 - 2018 | https://doi.org/10.3389/fmicb.2018.00358
Ocean ecosystems are inhabited by a diverse set of viruses that impact microbial mortality and evolution. However, the distribution abundances specific viral lineages, particularly those from large bank rare viruses, remains largely unknown. Here, we assessed diversity abundance TIM5-like cyanophages. The sequencing three new cyanophage genomes environmental amplicons signature gene Red Sea revealed highly conserved content sequence similarity. We adapted polony method, solid-phase...
Abstract Photosynthesis fuels primary production at the base of marine food webs. Yet, in many surface ocean ecosystems, diel-driven is tightly coupled to daily loss. This tight coupling raises question: which top-down drivers predominate maintaining persistently stable picocyanobacterial populations over longer time scales? Motivated by high-frequency water measurements taken North Pacific Subtropical Gyre (NPSG), we developed multitrophic models investigate bottom-up and mechanisms...