A5050390516- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Plant Virus Research Studies
- Wastewater Treatment and Nitrogen Removal
- Marine Biology and Ecology Research
- Methane Hydrates and Related Phenomena
- Evolution and Genetic Dynamics
- Aquatic Ecosystems and Phytoplankton Dynamics
- Environmental DNA in Biodiversity Studies
- Biocrusts and Microbial Ecology
- Photosynthetic Processes and Mechanisms
- Ethics in medical practice
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Odor and Emission Control Technologies
- Respiratory viral infections research
- Ethics in Business and Education
- Marine and coastal plant biology
- Diatoms and Algae Research
- Coral and Marine Ecosystems Studies
- Algal biology and biofuel production
- Vibrio bacteria research studies
Technion – Israel Institute of Technology
2016-2025
Case Western Reserve University
2012
Faculty (United Kingdom)
2012
University of Nebraska–Lincoln
2012
Massachusetts Institute of Technology
2002-2010
Interuniversity Institute for Marine Sciences in Eilat
1995-2006
Hebrew University of Jerusalem
1998-2006
IIT@MIT
2006
Woods Hole Oceanographic Institution
2004
Comparative genomics gives us a new window into phage-host interactions and their evolutionary implications. Here we report the presence of genes central to oxygenic photosynthesis in genomes three phages from two viral families (Myoviridae Podoviridae) that infect marine cyanobacterium Prochlorococcus. The encode photosystem II core reaction center protein D1 (psbA), high-light-inducible (HLIP) (hli) are present all genomes. Both myoviruses contain additional hli gene types, one them...
Cyanophages (cyanobacterial viruses) are important agents of horizontal gene transfer among marine cyanobacteria, the numerically dominant photosynthetic organisms in oceans. Some cyanophage genomes carry and express host-like photosynthesis genes, presumably to augment host machinery during infection. To study prevalence evolutionary dynamics this phenomenon, 33 cultured cyanophages known family range viral DNA from field samples were screened for presence two core photosystem reaction...
Prochlorococcus is the numerically dominant primary producer in open ocean ecosystems. Analysis of genome sequences from cultured isolates and samples has broadened interest studying this tiny cell, efforts are underway to develop it into a model system for marine microbial ecology. A critical component these been development systematic culturing methods that will facilitate distribution diverse labs may be interested it. This paper provides detailed maintaining cultures , including...
The seasonal dynamics of ultraphytoplankton for the northern Gulf Aqaba (29°28′N, 34°55′E) were investigated in detail. Monthly analysis pigments by HPLC and cell abundances epifluorescence microscopy showed large fluctuations community structure concurrent with strong changes water‐column conditions. Following extensive winter mixing, succession progressed rapidly as stratification strengthened. Eucaryotic algae dominated nutrient‐replete mixing conditions, Synechococcus was major component...
The marine cyanobacterium Prochlorococcus MED4 has the smallest genome and cell size of all known photosynthetic organisms. Like phototrophs at temperate latitudes, it experiences predictable daily variation in available light energy which leads to temporal regulation partitioning key cellular processes. To better understand tempo choreography this minimal phototroph, we studied entire transcriptome over a simulated light-dark cycle, placed context diagnostic physiological cycle parameters....
Professional identity and competent ethical behaviors of nursing students are commonly developed through curricular inclusion professional values education. Despite the enactment this approach, continue to express difficulty in managing conflicts encountered their practice. This descriptive correlational study explores relationships between values, self-esteem, decision making among senior baccalaureate students. A convenience sample 47 from United States were surveyed for level internalized...
Viruses infecting bacteria (phages) are thought to greatly impact microbial population dynamics as well the genome diversity and evolution of their hosts. Here we report on discovery a novel lineage tailed dsDNA phages belonging family Myoviridae describe its first representative, S-TIM5, that infects ubiquitous marine cyanobacterium, Synechococcus . The this phage encodes an entirely unique set structural proteins not found in any currently known phage, indicating it uses lineage-specific...
Prochlorococcus and Synechococcus are the dominant primary producers in marine ecosystems perform a significant fraction of ocean carbon fixation. These cyanobacteria interact with diverse microbial community that coexists them. Comparative genomics cultivated isolates has helped address questions regarding patterns evolution diversity among microbes, but can be is miniscule compared to wild. To further probe these groups extend utility reference sequence databases, we report data set single...
Long-term coexistence between unicellular cyanobacteria and their lytic viruses (cyanophages) in the oceans is thought to be due presence of sensitive cells which cyanophages reproduce, ultimately killing cell, while other survive resistance infection. Here, we investigated marine from genera Synechococcus Prochlorococcus compared modes against specialist generalist belonging T7-like T4-like cyanophage families. Resistance was extracellular most interactions irrespective phage family,...
Abstract The photosynthetic picocyanobacteria Prochlorococcus and Synechococcus are models for dissecting how ecological niches defined by environmental conditions, but interactions with bacteriophages affect picocyanobacterial biogeography in open ocean biomes has rarely been assessed. We applied single-virus single-cell infection approaches to quantify cyanophage abundance infected 87 surface water samples from five transects that traversed approximately 2,200 km the North Pacific Ocean on...
Abstract Siderophores are strong iron‐binding molecules produced and utilized by microbes to acquire the limiting nutrient iron (Fe) from their surroundings. Despite importance as a component of ligand pool in seawater, data on distribution siderophores that use them limited. Here, we measured concentrations types dissolved during two cruises April 2016 June 2017 transited iron‐replete, low‐macronutrient North Pacific Subtropical Gyre through Transition Zone (NPTZ) iron‐deplete,...
Prochlorococcus, an extremely small cyanobacterium that is very abundant in the world's oceans, has a streamlined genome. On average, these cells have about 2,000 genes and few regulatory proteins. The limited capability of regulation thought to be result selection imposed by relatively stable environment combination with Furthermore, only ten non-coding RNAs (ncRNAs), which play crucial roles all forms life, been described Prochlorococcus. Most strains also lack RNA chaperone Hfq, raising...
RNA turnover plays an important role in the gene regulation of microorganisms and influences their speed acclimation to environmental changes. We investigated whole-genome stability Prochlorococcus, a relatively slow-growing marine cyanobacterium doubling approximately once day, which is extremely abundant oceans. Using combination microarrays, quantitative RT-PCR new fitting method for determining decay rates, we found median half-life 2.4 minutes rate 2.6 expressed genes - twofold faster...
The ecologically important cyanobacterium Prochlorococcus possesses the smallest genome among oxyphototrophs, with a reduced suite of protein regulators and disproportionately high number regulatory RNAs.Many these are asRNAs, raising question whether they modulate gene expression through protection mRNA from RNase E degradation.To address this question, we produced recombinant sp.MED4, which functions optimally at 12 mM Mg 2+ , pH 9 35 C. cleavage assays were performed to assess enzyme...
Bacteria and their viruses (phages) are antagonists, yet have coexisted in nature for billions of years. Models proposed to explain the paradox antagonistic coexistence generally reach two types solutions: Arms race-like dynamics that lead hosts with increasing resistance infection ranges; population fluctuations between diverse host viral due a metabolic cost resistance. Recently, we found populations marine cyanobacterium, Prochlorococcus, consist cells extreme hypervariability gene...