A5073145057- Nuclear Structure and Function
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA regulation and disease
- Ubiquitin and proteasome pathways
- Advanced Electron Microscopy Techniques and Applications
- Amyotrophic Lateral Sclerosis Research
- Endoplasmic Reticulum Stress and Disease
- Biotin and Related Studies
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- Mitochondrial Function and Pathology
- Neurogenetic and Muscular Disorders Research
- Software Reliability and Analysis Research
- Software Engineering Research
- Microtubule and mitosis dynamics
- Probiotics and Fermented Foods
- DNA Repair Mechanisms
- Virus-based gene therapy research
- Genetics and Neurodevelopmental Disorders
- Machine Learning in Bioinformatics
- Antibiotic Use and Resistance
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Cerebrovascular and genetic disorders
Bar-Ilan University
2015-2025
Tel Aviv University
2022
Park Plaza Hospital
2022
Sheba Medical Center
2022
Technion – Israel Institute of Technology
2004-2012
University of Nebraska–Lincoln
2012
University of California, San Diego
1999-2008
Hebrew University of Jerusalem
1989-2000
Jerusalem College of Technology
1999
GeneCards (www.genecards.org) is a comprehensive, authoritative compendium of annotative information about human genes, widely used for nearly 15 years. Its gene-centric content automatically mined and integrated from over 80 digital sources, resulting in web-based deep-linked card each >73 000 gene entries, encompassing the following categories: protein coding, pseudogene, RNA gene, genetic locus, cluster uncategorized. We now introduce Version 3, featuring speedy sophisticated search...
Assembly of the nuclear pore, gateway to genome, from its component subunits is a complex process. In higher eukaryotes, pore assembly begins with binding ELYS/MEL-28 chromatin and recruitment large critical Nup107-160 subunit. The choreography steps that follow largely speculative. Here, we set out molecularly define early in assembly, beginning binding. Point mutation analysis indicates exquisitely sensitive change only two amino acids AT-hook motif ELYS. dependence on AT-rich for ELYS...
Viruses infecting bacteria (phages) are thought to greatly impact microbial population dynamics as well the genome diversity and evolution of their hosts. Here we report on discovery a novel lineage tailed dsDNA phages belonging family Myoviridae describe its first representative, S-TIM5, that infects ubiquitous marine cyanobacterium, Synechococcus . The this phage encodes an entirely unique set structural proteins not found in any currently known phage, indicating it uses lineage-specific...
Assembly of a eukaryotic nucleus involves three distinct events: membrane recruitment, fusion to form double nuclear membrane, and pore complex (NPC) assembly. We report that importin beta negatively regulates two these events, NPC When excess is added full Xenopus reconstitution reaction, vesicles are recruited chromatin but their blocked. The down-regulation Ran-GTP reversible. Indeed, RanGTP (RanQ69L) alone stimulates excessive fusion, leading intranuclear tubules cytoplasmic annulate...
In multicellular organisms, the higher order organization of chromatin during interphase and reassembly nuclear envelope mitosis are thought to involve an interaction between lamina chromatin. The distribution lamins peripheral is highly correlated in vivo , bind specifically vitro . Deletion mutants Drosophila lamin Dm 0 were expressed map regions protein that required for its binding chromosomes. activity requires two tail domain. apparent K d domain was found be approximately 1 μM....
ABSTRACT Using monoclonal antibodies, we followed the fate of three different nuclear envelope proteins during mitosis in Drosophila early embryos by indirect immunofluorescence microscopy. Two these proteins, lamin and otefin, a newly characterized polypeptide with an apparent Mr 53 000, are apparently present envelope-like structure that is throughout mitosis. Immunoelectron microscopy interphase nuclei indicates like lamin, not component pore complexes. In contrast gpl88, putative complex...
The nuclear envelope of higher eukaryotic cells reforms at the exit from mitosis, in concert with assembly pore complexes (NPCs). first step postmitotic NPC involves "seeding" chromatin ELYS and Nup107-160 complex. Subsequent steps process are poorly understood different mechanistic models have been proposed to explain formation full supramolecular structure. Here, we show that initial seeding is negatively regulated by importin beta. Direct imaging attachment sites reveals single situated...
ABSTRACT We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill mycelia of zygomycete molds. This migration was restricted molds several basidiomycete species. No seen on any phylum Ascomycota. S. did not require fungal viability or surrounding growth medium, as bacteria migrated aerial hyphae well. exhibit tropism toward mycelium. Bacterial proceeded only when grew into bacterial colony. cells initially hyphae,...
Abstract Nuclear envelopathies comprise a heterogeneous group of diseases caused by mutations in genes encoding nuclear envelope proteins. Mutations affecting lamina-associated polypeptide 1 (LAP1) result two discrete phenotypes muscular dystrophy and progressive dystonia with cerebellar atrophy. We report 7 patients presenting at birth severe neurological impairment, bilateral cataract, growth retardation early lethality. All the are homozygous for nonsense mutation TOR1AIP1 gene resulting...
The role of the Drosophila lamin protein in nuclear envelope assembly was studied using a vitro system that reconstitutes nuclei from added sperm chromatin or naked DNA. Upon incubation embryonic extract with anti-Drosophila antibodies, attachment membrane vesicles to surface and formation did not occur. Lamina were inhibited only when activity 75-kD isoform both soluble membrane-vesicles fractions. Incubation decondensed an depleted membranes revealed presence molecules on periphery. In...
Importin-β is the main vector for interphase nuclear protein import and plays roles after envelope breakdown. Here we show that importin-β regulates multiple aspects of mitosis via distinct domains interact with different classes proteins in human cells. The C-terminal region (which binds importin-α) inhibits mitotic spindle pole formation. central (harboring nucleoporin-binding sites) microtubule dynamic functions interaction kinetochores. interacts through this NUP358/RANBP2, which turn...
Neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), and Huntington's disease (HD), are characterized by intracellular aggregation of proteins. In the case ALS FTD, these protein aggregates found in cytoplasm affected neurons contain certain RNA-binding proteins (RBPs), namely TAR DNA-binding 43 kDa (TDP-43) fused sarcoma (FUS) gene product. TDP-43 FUS nuclear their displacement to is thought be adverse at least two ways: loss-of-function...
The nuclear matrix maintains specific interactions with genomic DNA at sites known as attachment regions (M/SARs). M/SARs bind in vitro to lamin polymers. We show that the polymerized α‐helical rod domain of Dm 0 provides by itself binding ftz M/SAR. In contrast, unpolymerized does not specifically this Non‐specific is also observed containing a point mutation impairs its ability polymerize or isolated tail domain. These data suggests lamins requires and depends on polymerization state.
Nuclear pore complexes (NPCs) are large proteinaceous channels embedded in double nuclear membranes, which carry out nucleocytoplasmic exchange. The mechanism of assembly involves a unique challenge, as it requires creation long-lived membrane-lined channel connecting the inner and outer membranes. This stabilized membrane has little evolutionary precedent. Here we mapped inner/outer fusion NPC biochemically by using novel intermediates inhibitors. Incubation Xenopus vitro system at 14°C...
Nuclear pore complexes (NPCs) are formed during two separate stages of the metazoan cell cycle. They assembled into re-forming nuclear envelope (NE) at exit from mitosis and an intact, expanding NE interphase. Here, we show that a soluble internal fragment membrane nucleoporin POM121 has dominant-negative effect on both modes assembly in cell-free reconstitution system. The binds chromatin sites distinct ELYS-Nup107-160 'seeding' prevents enclosure NPC formation. Importin-β negatively...
The 26S proteasome is a conserved 2.5 MDa protein degradation machine that localizes to different cellular compartments, including the nucleus. Little known about specific targeting mechanisms of proteasomes in eukaryotic cells. We used cell-free nuclear reconstitution system test for and import distinct species. Three types stable, proteolytically active particles were purified from Xenopus egg cytosol. Two these, holoenzyme 20S core particle, targeted periphery but did not reach...