A5103167219- Amyotrophic Lateral Sclerosis Research
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Conducting polymers and applications
- Genetics, Bioinformatics, and Biomedical Research
- Nerve injury and regeneration
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Neurogenetic and Muscular Disorders Research
- GABA and Rice Research
- Neuroscience and Neural Engineering
- DNA and Nucleic Acid Chemistry
- Computational Drug Discovery Methods
- CRISPR and Genetic Engineering
- Lipid Membrane Structure and Behavior
- Genetic Neurodegenerative Diseases
- Prion Diseases and Protein Misfolding
- Neurogenesis and neuroplasticity mechanisms
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Genomics, phytochemicals, and oxidative stress
- Connexins and lens biology
- Immunotherapy and Immune Responses
- Planarian Biology and Electrostimulation
- Caveolin-1 and cellular processes
ReproCELL (United States)
2021-2024
Johns Hopkins Medicine
2012-2022
Johns Hopkins University
2012-2022
Kennedy Krieger Institute
2012
National Institutes of Health
2008-2011
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2011
Centre National de la Recherche Scientifique
2005-2007
Université de Montpellier
2005-2007
Cellular uptake of a family cationic cell-penetrating peptides (examples include Tat and penetratin) have been ascribed in the literature to mechanism that does not involve endocytosis. In this work we reevaluate mechanisms cellular 48-60 (Arg)(9). We demonstrate here cell fixation, even mild conditions, leads artifactual these peptides. Moreover, show flow cytometry analysis cannot be used validly evaluate unless step trypsin digestion membrane-adsorbed peptide is included protocol....
Delivery of macromolecules mediated by protein transduction domains (PTDs) attracts a lot interest due to its therapeutic and biotechnological potential. A major reevaluation the mechanism PTD-mediated internalization role endocytosis in this has been recently initiated. Here, we demonstrate that entry TAT peptide (one most widely used PTDs) into different primary cells is ATPand temperature-dependent, indicating involvement endocytosis. Specific inhibitors clathrin-dependent partially...
Duchenne muscular dystrophy (DMD) remains an intractable genetic disease. Althogh there are several animal models of DMD, is no human cell model that carries patient-specific DYSTROPHIN mutations. Here, we present a DMD using induced pluripotent stem cells (hiPSCs). Our reveals concordant disease-related phenotypes with patient-dependent variation, which partially reversed by and pharmacological approaches. "chemical-compound-based" strategy successfully directs hiPSCs into expandable...
Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive loss of motor neurons in the CNS. Astrocytes play critical role progression ALS. are interconnected through family gap junction proteins known as connexins (Cx). Cx43 major astrocyte connexin conducting crucial homeostatic functions Under pathological conditions, expression and altered. Here we report that an abnormal increase serves one mechanisms for astrocyte‐mediated toxicity We observed SOD1...
Connexin 43 (Cx43) gap junctions and hemichannels mediate astrocyte intercellular communication in the central nervous system under normal conditions contribute to astrocyte-mediated neurotoxicity amyotrophic lateral sclerosis (ALS). Here, we show that astrocyte-specific knockout of Cx43 a mouse model ALS slows disease progression both spatially temporally, provides motor neuron (MN) protection, improves survival. In addition, expression is up-regulated human postmortem tissue cerebrospinal...
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder marked by the loss of motor neurons (MNs) in brain and spinal cord, leading to fatally debilitating weakness. Because this disease predominantly affects MNs, we aimed characterize distinct expression profile that cell type elucidate underlying mechanisms identify novel targets inform on MN health during ALS time course. microRNAs (miRNAs) are short, noncoding RNAs can shape thus often exhibit cell-type-enriched...
The efficacy of drugs targeting the CNS is influenced by their limited brain access, which can lead to complete pharmacoresistance. Recently a tissue-specific and selective upregulation multidrug efflux transporter ABCB1 or P-glycoprotein (P-gp) in spinal cord both patients mutant SOD1-G93A mouse model amyotrophic lateral sclerosis (ALS), fatal neurodegenerative disease that prevalently kills motor neurons has been reported. Here, we extended analysis P-gp expression ALS found was restricted...
Abstract The ability to generate human-induced pluripotent stem cell (hiPSC)-derived neural cells displaying region-specific phenotypes is of particular interest for modeling central nervous system biology in vitro. We describe a unique method by which spinal cord hiPSC-derived astrocytes (hiPSC-A) are cultured with motor neurons (hiPSC-MN) multielectrode array (MEA) record electrophysiological activity over time. show that hiPSC-A enhance hiPSC-MN maturation time-dependent fashion. sequence...
Spinal cord motor neurons (MNs) from human iPS cells (iPSCs) have wide applications in disease modeling and therapeutic development for amyotrophic lateral sclerosis (ALS) other MN-associated neurodegenerative diseases. We need highly efficient MN differentiation strategies generating iPSC-derived models that closely recapitulate the genetic phenotypic complexity of ALS. An important application these is to understand molecular mechanisms action FDA-approved ALS drugs only show modest...
Abstract Sensory neurons are afferent in sensory systems that convert stimuli and transmit information to the central nervous system as electrical signals. Primary affected by non-noxious noxious present dorsal root ganglia (DRG), DRG used an vitro model of nociceptive response. However, derived from mouse or rat give a low yield neurons, they difficult culture. To help alleviate this problem, we characterized human induced pluripotent stem cell (hiPSC) neurons. They can solve problems...
Cell-to-cell fusion plays an important role in normal physiology and different pathological conditions. Early stages mediated by specialized proteins yielding pores are followed a pore expansion stage that is dependent on cell metabolism yet unidentified machinery. Because of similarity membrane bending the rim highly curved intracellular compartments, present study we explored whether changes activity generate these compartments affect initiated protein fusogens influenza virus baculovirus....
Abstract The generation of human induced pluripotent stem cells (hiPSCs) represents an exciting advancement with promise for cell transplantation therapies as well neurological disease modeling. Based on the emerging roles astrocytes in disorders, we investigated whether hiPSC-derived astrocyte progenitors could be engrafted to rodent spinal cord and how characteristics these changed between vitro culture after vivo environment. Our results show that embryonic cell- survive long-term...
Tumor‐initiating stem cells (also referred to as cancer cells, CSCs ) are a subpopulation of that play unique roles in tumor propagation, therapeutic resistance and recurrence. It is increasingly important understand how molecular signaling regulates the self‐renewal differentiation . Basic helix‐loop‐helix (b HLH transcription factors critical for normal yet their neoplastic not well understood. In glioblastoma neurosphere cultures contain (GBM‐CSCs), b family member inhibitors DNA binding...
Abstract Background Non-targeted cytotoxics with anticancer activity are often developed through preclinical stages using response criteria observed in cell lines and xenografts. A panel of the NCI-60 is frequently first line to define tumor types that optimally responsive. Open data on gene expression lines, provides a unique opportunity add another dimension development such drugs by interrogating correlations patterns. Machine learning can be used reduce complexity whole genome patterns...
Abstract Connexin 43 (Cx43) gap junctions and hemichannels mediate astrocyte intercellular communication in the central nervous system under normal conditions may contribute to astrocyte-mediated neurotoxicity amyotrophic lateral sclerosis (ALS). Here we show that astrocyte-specific knockout of Cx43 a mouse model ALS slows disease progression both spatially temporally, provides motor neuron (MN) protection, improves survival. In human tissues biofluids, observe higher levels correlate with...
// Aditya Kulkarni 1 , Joseph Ryan McDermott Umesh Kathad Rama Modali 2 Jean-Philippe Richard Panna Sharma and Kishor Bhatia Lantern Pharma, Inc., Dallas, TX 75201, USA REPROCELL Beltsville, MD 20705, Correspondence to: Kulkarni, email: aditya@lanternpharma.com Keywords: non-small cell lung cancer; acylfulvene; alkylating agent; PTGR1; LP-184 Received: January 11, 2021 Accepted: March 29, Published: April 13, Copyright: © et al. This is an open access article distributed under the terms of...