ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification of the two major forms rat pituitary corticotropin using only reversed-phase liquid chromatographyH. P. J. Bennett, C. A. Browne, and S. SolomonCite this: Biochemistry 1981, 20, 16, 4530–4538Publication Date (Print):August 1, 1981Publication History Published online1 May 2002Published inissue 1 August 1981https://pubs.acs.org/doi/10.1021/bi00519a004https://doi.org/10.1021/bi00519a004research-articleACS PublicationsRequest reuse...

Abstract The relative effectiveness of trifluoroacetic acid (TFA), pentafluoropropanoic (PFPA), heptafluorobutyric (HFBA) and undecafluorocaproic (UFCA) as hydrophobic counter-ions in the reversedphase high performance liquid chromatography (RP-HPLC) peptides was assessed. Four solvent systems were compared each containing 0.01M a perfluorocarboxylic throughout. Twelve standard proteins loaded onto RP-HPLC column which eluted with linear gradient 20-58.4% aqueous acetonitrile over 90...

Peptides can be adsorbed on octadecasilyl-silica from large volumes of aqueous solution and eluted with solvent mixtures containing methanol or acetonitrile. These properties may used for the extraction purification peptide fragments in plasma samples collected rats. After intravenous injection Synacthen [corticotropin-(1-24)-tetracosapeptide], it was shown that within 2 min main circulating products were intact its sulphoxide. In addition, a number indicative cleavage at N- C-termini...

The neurohypophyseal hormone oxytocin (OT) is the most potent uterotonic agent known and used to induce labor. Yet, endogenous circulating OT appears not participate in induction of As shown here, finding messenger RNA peptide uterus suggests a solution for this paradox. During gestation, rat increased more than 150-fold and, at term, exceeded hypothalamic by 70-fold. Thus, during parturition, may act primarily as local mediator hormone.

Abstract Background Progranulin is a secreted high molecular weight growth factor bearing seven and one half copies of the cysteine-rich granulin-epithelin motif. While inappropriate over-expression progranulin gene has been associated with many cancers, haploinsufficiency leads to atrophy frontotemporal lobes development form dementia (frontotemporal lobar degeneration ubiquitin positive inclusions, FTLD-U) formation ubiquitinated inclusions. Recent reports indicate that neurotrophic...

Progranulin is a secreted protein with important functions in several physiological and pathological processes, such as embryonic development, host defense, wound repair. Autosomal dominant mutations the progranulin gene cause frontotemporal dementia, while overexpression of promotes invasive progression range tumors, including those breast brain. Structurally, consists seven-and-a-half tandem repeats granulin/epithelin module (GEM), which have been isolated discrete 6-kDa GEM peptides. We...

Batch soil washing experiments were performed to evaluate the feasibility of using surfactin from Bacillus subtilis, a lipopeptide biosurfactant, for removal heavy metals contaminated and sediments. The contained high levels hydrocarbons (890 mg/kg zinc, 420 copper, 12.6% oil grease), sediments 110 copper 3300 zinc. was spiked increase cadmium 550, 1200, 2000 mg/kg, respectively. Water alone removed minimal amounts zinc (less than 1%). Results showed that 0.25% surfactin/1% NaOH could remove...

Mutations in the PEX gene are responsible for X-linked hypophosphatemic rickets. To gain insight into role of normal physiology we have cloned human full-length cDNA and studied its tissue expression, subcellular localization, peptidase activity. We show that encodes a 749-amino acid protein structurally related to family neutral endopeptidases include neprilysin as prototype. By Northern blot analysis, size transcript is 6.5 kilobases. determined by semi-quantitative polymerase chain...

Abstract Neural apoptosis‐regulated convertase‐1/proprotein convertase subtilisin‐kexin like‐9 (NARC‐1/PCSK9) is a proprotein recently described to play major role in cholesterol homeostasis through enhanced degradation of the low‐density lipoprotein receptor (LDLR) and possibly neural development. Herein, we investigated potential involvement this proteinase development CNS using mouse embryonal pluripotent P19 cells zebrafish as models. Time course quantitative RT–PCR analyses were...

We examined the characteristics of mitogens extracted from human benign prostatic hyperplasia and adenocarcinoma tissue. Although for fetal rat skin fibroblasts as well calvarial osteoblasts osterosarcoma cells were found, distinct entities that acted selectively in osteoblast phenotype could be obtained by sequential reverse-phase high performance liquid chromatography. Two peptides with apparent molecular weights 10,000 13,000 D derived hyperplastic tissue, whereas a single moiety was...

We have purified peptides with PTH-like bioactivity from a rat Leydig cell tumor (H-500) and human squamous carcinoma, both associated syndrome of humorinduced hypercalcemia. Tumor extracts were shown to be active in an vitro renal cytochemical bioassay osteosarcoma (UMR 108) adenylate cyclase assay; activity assays could reduced by the PTH antagonist [norleucine-8,18,tyrosine-34]bovine PTH-(3–34)-amide. Partially tumors tumor-conditioned culture medium vivo thyroparathyroidectomized rats...

Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed adsorption the supernatant on small bed of octadecasilyl-silica. Recoveries corticotropins and somatostatin added to variety were quantitative, peptides undamaged as determined high-pressure liquid chromatography.

A novel procedure utilizing reversed-phase high-performance liquid chromatography for the extraction and purification of peptides from biological tissues has been applied to isolation corticotropin-like intermediary lobe peptide (CLIP) alpha-melanocyte-stimulating hormone (alpha-MSH) neurointermediary rat pituitary. The characterization two major forms CLIP alpha-MSH are described. isolated have identified by using enzymatic digestions mapping. main form ClIP is a which modified...

An adrenocorticotrophic hormone (ACTH) was isolated from extracts of the pars distalis pituitary dogfish Squalus acanthias by gel filtration and ion-exchange chromatography. It had 15% potency human ACTH in promoting cortico-steroidogenesis rat adrenal cells. Sequence analysis revealed it to be a nonatria-contapeptide with following primary structure:...

A melanocyte-stimulating hormone (MSH) has been isolated from extracts of the neurointermediate lobe pituitary dogfish Squalus acanthias by gel-filtration and ion-exchange chromatography. It had approximately 1% potency mammalian alpha-MSH on bioassays in vitro frog skin skin. Sequence analysis revealed it to be a hexadecapeptide with following primary structure: Asp-Gly-Asp-Asp-Tyr-Lys-Phe-Gly-His-Phe-Arg-Trp-Ser-Val-Pro-Leu. appears related beta-MSH species but only sequence...

Progranulin is a secreted growth factor that active in tumorigenesis, wound repair, and inflammation. Haploinsufficiency of the human progranulin gene, GRN, causes frontotemporal dementia. Progranulins are composed chains cysteine-rich granulin modules. Modules may be released from by proteolysis as 6kDa polypeptides. Both intact some polypeptides biologically active. The module occurs certain plant proteases progranulins present early diverging metazoan clades such sponges, indicating their...