A5057049348- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Scientific Computing and Data Management
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Molecular Biology Techniques and Applications
Centre for Genomic Regulation
2018-2025
Universitat Pompeu Fabra
2018-2024
Institute of Science and Technology
2018
The increasing number of biomedical and translational applications in mass spectrometry-based proteomics poses new analytical challenges raises the need for automated quality control systems. Despite previous efforts to set standard file formats, data processing workflows key evaluation parameters control, systems are not yet widespread among laboratories, which limits acquisition high-quality results, inter-laboratory comparisons assessment variability instrumental platforms. Here we...
QCloud is a cloud-based system to support proteomics laboratories in daily quality assessment using user-friendly interface, easy setup, and automated data processing. Since its release, has facilitated control for experiments many laboratories. provides quick effortless evaluation of instrument performance that helps overcome analytical challenges derived from clinical translational research. Here we present an improved version the system, QCloud2. This new includes enhancements scalability...
Abstract Mass spectrometry (MS)-based proteomics is known for its high accuracy in quantifying peptides and proteins using various calibration strategies, including internal external curves. While multi-point curves are created from serial dilutions, they often fail to account sample-specific matrix effects. In contrast, sample but face scalability cost challenges whole proteome analyses. this manuscript we present a novel TMT-based multipoint curve strategy, referred as TMTCal, which...
The need for a better understanding of cellular heterogeneity has pushed mass spectrometry technologies to the analysis single-cell and single-cell-type proteomes, although several challenges still limit their widespread implementation. Among efforts toward low-input analyses, there is adoption data-independent acquisition methods increase analytical sensitivity. Here, we revisited use linear ion traps analyzers in demonstrate benefits boost peptide protein identifications proteomes.
Targeted proteomics has become the method of choice for biomarker validation in human biopsies due to its high sensitivity, reproducibility, accuracy, and precision. However, targeted be transferred clinical routine there is need reduce complexity, make procedures simpler, increase throughput, improve analytical performance. Here we present Isotopologue Multipoint Calibration (ImCal) quantification strategy, which uses a mix isotopologue peptides generate internal multipoint calibration...
Abstract Cerebrospinal fluid (CSF) is a valuable liquid biopsy for identifying protein biomarkers in neurological diseases, yet its proteome profiling faces challenges due to the large dynamic range of abundances. In this study, we assessed effectiveness commercial enrichment strategy, initially developed plasma samples, enhancing detection low-abundance proteins human CSF. We demonstrate significant improvements identification and coverage depth while maintaining high reproducibility low...