A5089751930- Mass Spectrometry Techniques and Applications
- Neurobiology and Insect Physiology Research
- Bacterial Identification and Susceptibility Testing
- Advanced Proteomics Techniques and Applications
- Ion-surface interactions and analysis
- Invertebrate Immune Response Mechanisms
- Astro and Planetary Science
- Physiological and biochemical adaptations
- Analytical Chemistry and Chromatography
- Biotin and Related Studies
- Hemoglobinopathies and Related Disorders
- Endoplasmic Reticulum Stress and Disease
- Amino Acid Enzymes and Metabolism
- Biosensors and Analytical Detection
- Protein Hydrolysis and Bioactive Peptides
- Retinoids in leukemia and cellular processes
- Neuroendocrine regulation and behavior
- Isotope Analysis in Ecology
- Primate Behavior and Ecology
- Crustacean biology and ecology
- Hemoglobin structure and function
- Parasite Biology and Host Interactions
- Metabolomics and Mass Spectrometry Studies
- Origins and Evolution of Life
- Chemical Synthesis and Analysis
Bruker (United States)
2003-2023
Harvard University
2009
University of Wisconsin–Madison
2009
Scripps Research Institute
1998
Texas A&M University
1997
Understanding the practical limitations of chemical reactions is critically important for efficiently planning synthesis compounds in pharmaceutical, agrochemical, and specialty research development. However, literature reports scope new are often cursory biased toward successful results, severely limiting ability to predict reaction outcomes untested substrates. We herein illustrate strategies carrying out large-scale surveys reactivity by using a material-sparing nanomole-scale automated...
A combined approach using mass spectrometry, a novel neuron affinity capture technique, and Drosophila melanogaster genetic manipulation has been developed to characterize the expression localization of neuropeptides in adult D. brain. In extract from whole brain, 42 18 peptide families were sequenced. Neuropeptide profiling also was performed on targeted populations cells which enriched with immunoaffinity purification genetically expressed marker.
A mass spectrometric method was used for the localization and sequence characterization of peptides in nervous system parasitic nematode Ascaris suum. Mass techniques utilizing MALDI-TOF, MALDI-TOF/TOF, MALDI-FT instruments were combined with situ chemical derivatization to examine expression known putative neuropeptides A. suum system. This first attempt at peptidomic mapped 39 neuropeptides, 17 which are considered be novel whose has not been previously reported. These analyses also...
We describe a novel nanostructured target plate for laser desorption/ionization (LDI) mass spectrometry, NALDI (Bruker Daltonics, Billerica, MA) plates. The active surface comprises several layers of inorganic materials that are structured at the nanoscale and then further coated with hydrophobic organic layer facilitates sample deposition LDI performance. These targets have been designed to analyze low (below 1500 Da), relatively polar, molecules they shown be up 10 times more sensitive...
Hemoglobinopathies are the most common inherited disorders in humans and thus target of screening programs worldwide. Over past decade, mass spectrometry (MS) has gained a more important role as clinical means to diagnose variants, number approaches have been proposed for characterization. Here we investigate use matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS) with sequencing using in-source decay (MALDI-ISD) characterization Hb variants. We explored effect...
Modification of arginine residues in bradykinin, [1-5]-bradykinin, splenopentin and two synthetic pentapeptides with acetylacetone (pentane-2,4-dione) significantly increases the relative abundance sequence-specific fragment ions produced by matrix-assisted laser desorption/ionization (MALDI). The fragmentation efficiency as measured post-source decay a reflectron time-of-flight mass spectrometer factor 2-3.5. Peptide bonds adjacent to modified are more susceptible cleavage than...
Abstract Direct analysis of tissue by matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) allows for the rapid profiling biological molecules with minimal loss sample or degradation and reduced likelihood chemical modification. However, there are still considerable challenges to overcome due complexity low quantity endogenous peptide in a single cell. These problems exacerbated nematode Ascaris suum because small size individual neurons paucity per In an effort address...
Matrix-assisted laser desorption/ionization time-of-flight and tandem (MALDI-TOF MALDI-TOF/TOF) mass spectrometry were used to sequence localize three novel, related neuropeptides in the nervous system of nematode Ascaris suum, AMRNALVRFamide (AF21), NGAPQPFVRFamide (AF22), SGMRNALVRFamide (AF23). The amino acid sequences clone a novel neuropeptide gene (afp-6) that encodes precursor bearing single copy each peptides. In situ hybridization immunocytochemistry revealed both transcript...
This paper characterizes the first protein to be discovered in a meteorite. Amino acid polymers previously observed Acfer 086 and Allende meteorites [1,2] have been further characterized via high precision MALDI mass spectrometry reveal principal unified structure of molecular weight 2320 Daltons that involves chains glycine hydroxy-glycine residues terminated by iron atoms, with additional oxygen lithium atoms. Signal-to-noise ratios up 135 allowed quantification various fragments isotope...
We report that polymer amide [1] with a protein backbone of mainly glycine units and iron is present in the CV3 meteorites Acfer 086, Allende KABA. The evidence for this from particles these after Folch extraction being analyzed by MALDI mass spectrometry 3D physical structures form various solvent phases. two forms we observe are branching rods entrapping spheres on 100micron scale. Two potential molecular presented report. One term hemolithin 2320Da, contains glycine, hydroxy-glycine, Fe,...
Secondary ion mass spectrometry (SIMS) applied in the event-by-event bombardment/detection mode is uniquely suited for characterization of individual nano-objects. In this approach, nano-objects are examined one-by-one, allowing detection variations composition. The validity analysis depends upon ability to physically isolate on a chemically inert support. This requirement can be realized by deposition Nano-Assisted Laser Desorption/Ionization (NALDI™) plate. featured nanostructured surface...
ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionAnalysis of Neuropeptide Expression and Localization in Adult Drosophila melanogaster Central Nervous System by Affinity Cell-Capture Mass SpectrometryJoanne Y. Yew, Yun Wang, Natasha Barteneva, Sergei Dikler, Kimberly K. Kutz-Naber, Lingjun Li, Edward A. KravitzCite this: J. Proteome Res. 2009, 8, 7, 3786Publication Date (Web):June 10, 2009Publication History Published online10 June...