A5032971187- Analytical Chemistry and Chromatography
- Protein purification and stability
- Microfluidic and Capillary Electrophoresis Applications
- Crystallization and Solubility Studies
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- X-ray Diffraction in Crystallography
- Innovative Microfluidic and Catalytic Techniques Innovation
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Chromatography in Natural Products
- Advanced Chemical Sensor Technologies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Free Radicals and Antioxidants
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Biochemical and Molecular Research
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Response and Inflammation
- PARP inhibition in cancer therapy
- Pharmacogenetics and Drug Metabolism
- Computational Drug Discovery Methods
- Enzyme Catalysis and Immobilization
- Ovarian cancer diagnosis and treatment
- RNA modifications and cancer
Merck & Co., Inc., Rahway, NJ, USA (United States)
2016-2025
The Ohio State University
2019-2024
Icahn School of Medicine at Mount Sinai
2024
Rutgers New Jersey Medical School
2023
Rutgers, The State University of New Jersey
2023
Ohio University
2023
Lung Institute
2020-2022
First Hospital of Jilin University
2019
Jilin University
2019
Understanding the practical limitations of chemical reactions is critically important for efficiently planning synthesis compounds in pharmaceutical, agrochemical, and specialty research development. However, literature reports scope new are often cursory biased toward successful results, severely limiting ability to predict reaction outcomes untested substrates. We herein illustrate strategies carrying out large-scale surveys reactivity by using a material-sparing nanomole-scale automated...
Organic electrosynthesis is a rapidly evolving field, providing powerful methods to assemble targets of interest in organic synthesis. Concerns around the scalability electrochemical remain biggest reason behind their scarce implementation manufacturing routes for pharmaceutical industry. To fill this gap, we report workflow describing key reaction parameters toward successful scale-up an electrosynthetic method from milligram kilogram scale. The used demonstrate our and flow setting was...
Enantioselective chromatography has been the preferred technique for determination of enantiomeric excess across academia and industry. Although sequential multicolumn enantioselective supercritical fluid screenings are widespread, access to automated ultra-high-performance liquid (UHPLC) platforms using state-of-the-art small particle size chiral stationary phases (CSPs) is an underdeveloped area. Herein, we introduce a UHPLC screening workflow capable combining 14 columns (packed with...
Tandem column liquid chromatography (LC) is a convenient, cost-effective approach to resolve multicomponent mixtures by serially coupling columns on readily available one-dimensional separation systems without specialized user training. Yet, adoption of this technique remains limited, mainly due the difficulty in identifying optimal selectivity out many possible tandem combinations. At point, method development and optimization require laborious “hit-or-miss” experimentation “blind”...
Recent developments in two-dimensional liquid chromatography (2D-LC) now make separation and analysis of very complex mixtures achievable. Despite being such a powerful chromatographic tool, current 2D-LC technology requires series arduous method development activities poorly suited for fast-paced industrial environment. introductions new technologies including active solvent modulation support multicolumn are helping to overcome this stigma. However, many practitioners believe that the lack...
At the forefront of chemistry and biology research, development timelines are fast-paced large quantities pure targets rarely available. Herein, we introduce a new framework, which is built upon an automated, online trapping-enrichment multi-dimensional liquid chromatography platform (TE-Dt-mDLC) that enables: 1) highly efficient separation complex mixtures in first dimension (1 D-UV); 2) automated peak buffer removal achieved through sequence H2 O D2 washes using independent pump setup; 3)...
The emergence of complex biological modalities in the biopharmaceutical industry entails a significant expansion current analytical toolbox to address need deploy meaningful and reliable assays at an unprecedented pace. Size exclusion chromatography (SEC) is standard technique for protein separation analysis. Some constraints traditional SEC stem from its restricted ability resolve mixtures notoriously long run times while also requiring multiple offline conditions on different pore size...
Continued adoption of two-dimensional liquid chromatography (2D-LC) in industrial laboratories will depend on the development approaches to make method for 2D-LC more systematic, less tedious, and reliant user expertise. In this paper, we build previous efforts these directions by describing use multifactorial modeling software that can help streamline simplify process 2D-LC. Specifically, have focused building retention models second dimension (2D) separations involving variables including...
The mounting complexity of new modalities in the biopharmaceutical industry entails a commensurate level analytical innovations to enable rapid discovery and development novel therapeutics vaccines. Hydrophobic interaction chromatography (HIC) has become one widely preferred separation techniques for analysis purification biopharmaceuticals under nondenaturing conditions. Inarguably, HIC method remains very challenging labor-intensive owing numerous factors that are typically optimized by...
Matrix-assisted laser desorption/ionization (MALDI) coupled with a time-of-flight (TOF) mass-spectrometry (MS) detector is acknowledged to be very useful for analysis of biological molecules. At the same time, hydrogen-deuterium exchange (HDX) well-known technique studying protein higher-order structure. However, coupling MALDI HDX has been challenging because undesired back-exchange reactions during analysis. In this report, we survey an approach that utilizes automated sample preparation...
An ene-reductase (ERED 36) with broad substrate specificity was identified, and optimization studies led to the development of an enzymatic protocol for reduction α,β-unsaturated acids under mild, aqueous conditions. The scope includes aromatic- aliphatic-substituted acrylic acids, as well cyclic α,β-substituted yielding chiral α-substituted exquisite levels enantioselectivity (>99% ee).
HIPK2 is a multifunctional kinase that acts as key pathogenic mediator of chronic kidney disease and fibrosis. It central effector multiple signaling pathways implicated in injury, such TGF-β/Smad3-mediated extracellular matrix accumulation, NF-κB-mediated inflammation, p53-mediated apoptosis. Thus, better understanding the specific regions necessary for distinct downstream pathway activation critical optimal drug development CKD. Our study now shows Caspase 6-mediated removal C-terminal...
The employment of ethylenediaminetetraacetic acid (EDTA) across several fields in chemistry and biology has required the creation a high number quantitative assays. Nonetheless, determination trace EDTA, especially biologics vaccines, remains challenging. Herein, we introduce an automated high-throughput approach based on EDTA esterification 96-well plates using boron trifluoride-methanol combined with rapid analysis by ultra-high-performance liquid chromatography–triple quadrupole tandem...