A5087287018- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Ubiquitin and proteasome pathways
Institut Européen de Chimie et Biologie
2015-2020
Institut de Biochimie et Génétique Cellulaires
2015-2020
Université de Bordeaux
2015
The presence of DNA double-strand breaks during mitosis is particularly challenging for the cell, as it produces broken chromosomes lacking a centromere. This situation can cause genomic instability resulting from improper segregation fragments into daughter cells. We recently uncovered process by which are faithfully transmitted via BubR1-dependent tethering two chromosome ends. However, mechanisms underlying BubR1 recruitment and function on were largely unknown. show that requires...
Chromatid segregation must be coordinated with cytokinesis to preserve genomic stability. Here we report that cells clear trailing chromatids from the cleavage site by undergoing two phases of cell elongation. The first phase relies on assembly a wide contractile ring. second requires activity pool myosin flows ring and enriches nascent daughter cortices. This efflux is novel feature its duration coupled nuclear envelope reassembly sequestration Rho-GEF Pebble. Trailing induce delay in...
The DNA damage sensor Mre11-Rad50-Nbs1 complex and Polo kinase are recruited to lesions during mitosis. However, their mechanism of recruitment is elusive. Here, using live-cell imaging combined with micro-irradiation single chromosomes, we analyze the dynamics Mre11 at mitosis in Drosophila These two proteins display distinct kinetics. Whereas kinetics double-strand breaks (DSBs) Cdk1-driven, promptly but briefly associates DSBs regardless phase re-associates proceeding interphase....