Abstract Geranylgeranyl diphosphate (GGPP) is the precursor for biosynthesis of gibberellins, carotenoids, chlorophylls, isoprenoid quinones, and geranylgeranylated proteins in plants. There a small gene family GGPP synthases encoding five isozymes one related protein Arabidopsis, all homologs have putative localization signal to translocate into specific subcellular compartments. Using synthetic green fluorescent (sGFP), we studied these synthases. When fusion were expressed by cauliflower...

Unlike ubiquitin, the ubiquitin-like protein modifier SUMO-1 and its budding yeast homologue Smt3p have been shown to be more important for posttranslational modification than degradation. Here we describe identification of fission yeast, which show required a number nuclear events including control telomere length chromosome segregation. A disruption pmt3(+) gene, Schizosaccharomyces pombe SMT3, was not lethal, but mutant cells carrying disrupted gene grew slowly. The pmt3Delta showed...

We developed a new series of Gateway binary vectors, R4pGWBs, that are plant transformation vectors designed for one-step construction chimeric genes between any promoter and cDNA. The structure R4pGWBs is almost the same as promoterless type improved pGWBs (ImpGWBs), except attR1 site replaced with attR4, which enables tripartite recombination these promoter- cDNA-entry clones. While ImpGWBs suitable analysis constitutive expression cDNAs in higher plants, have great advantage expressing...

We have identified, cloned, and studied a gene, cap, encoding protein that is associated with adenylyl cyclase in the fission yeast Schizosaccharomyces pombe. This shares significant sequence homology cyclase-associated CAP Saccharomyces cerevisiae. bifunctional protein; N-terminal domain appears to be involved cellular responsiveness RAS, whereas loss of C-terminal portion morphological nutritional defects. S. pombe cap can suppress phenotypes deletion cerevisiae but does not domain....

Abstract Fungal cell walls are medically important since they represent a drug target site for antifungal medication. So far there is no method to directly visualize structurally similar wall components such as α-glucan, β-glucan and mannan with high specificity, especially in label-free manner. In this study, we have developed Raman spectroscopy based molecular imaging combined multivariate curve resolution analysis enable detection visualization of multiple polysaccharide simultaneously at...

The Escherichia coli ispB gene encoding octaprenyl diphosphate synthase is responsible for the synthesis of side chain isoprenoid quinones. We tried to construct an E. ispB-disrupted mutant but could not isolate chromosomal disrupted unless or its homolog was supplied on a plasmid. disruptants that harbored plasmids carrying homologs from Haemophilus influenzae and Synechocystis sp. strain PCC6803 produced mainly ubiquinone 7 9, respectively. Our results indicate function essential normal...

The nucleotide sequence of the Escherichia coli mhpB gene, encoding 2,3-dihydroxyphenylpropionate 1,2-dioxygenase, was determined by sequencing a 3.1-kb fragment DNA from Kohara phage 139. inferred amino acid showed 58% identity with an extradiol dioxygenase, MpcI, Alcaligenes eutrophus and 10 to 20% protocatechuate 4,5-dioxygenase Pseudomonas paucimobilis, 3,4-dihydroxyphenylacetate 2,3-dioxygenase E. coli, human 3-hydroxyanthranilate dioxygenase. Sequence similarity between N- C-terminal...

The isoprenoid chain of ubiquinone (Q) is determined by trans-polyprenyl diphosphate synthase in micro-organisms and presumably mammals. Because mice humans produce Q9 Q10, they are expected to possess solanesyl decaprenyl synthases as the determining enzyme for a type ubiquinone. Here we show that murine human heterotetramers composed newly characterized hDPS1 (mSPS1) hDLP1 (mDLP1), which have been identified orthologs Schizosaccharomyces pombe Dps1 Dlp1, respectively. Whereas or mSPS1 can...

The adenylyl cyclase gene, cyr1, of Schizosaccharomyces pombe has been cloned. We have begun an analysis the function and regulation by disrupting this gene over-expressing all or parts in various strains. cyr1- strains are viable contain no measurable cyclic AMP. They conjugate sporulate under conditions that normally inhibit wild-type Strains containing cyr1 coding sequences transcribed from strong adh1 promoter greatly elevated activity, as measured vitro, but only modestly cAMP levels....

Homozygous mice carrying kd (kidney disease) mutations in the gene encoding prenyl diphosphate synthase subunit 2 ( Pdss2 kd/kd ) develop interstitial nephritis and eventually die from end-stage renal disease. The PDSS2 polypeptide concert with PDSS1 synthesizes polyisoprenyl tail of coenzyme Q (Q or ubiquinone), a lipid quinone required for mitochondrial respiratory electron transport. We have shown that deficiency content is evident mouse kidney extracts by 40 days age thus precedes onset...

Abstract Some marine fishes are algae-feeding, and the microorganisms in their digestive tracts produce carbohydrate hydrolyzing enzymes such as agarose fucosidase, which potentially interesting resource for new functional enzymes. The purpose of this study was to establish a method identifying utilizing characteristic bacteria from intestines two algae-eating fish species: Andamia tetradactylus , exclusively eats algae on rock surface, stellar rockskipper Entomacrodus stellifer feeds both...

ABSTRACT The fission yeast Schizosaccharomyces pombe is an excellent model organism to explore cellular events owing rich tools in genetics, molecular biology, and biochemistry. proliferates continuously when nutrients are abundant but arrests G1 phase upon depletion of such as nitrogen glucose. When cells opposite mating types present, conjugate, fuse, undergo meiosis, finally form 4 spores. This sexual differentiation process S. has been studied extensively. To execute differentiation, the...

Schizosaccharomyces pombe produces ubiquinone-10 whose side chain is thought to be provided by the product generated decaprenyl diphosphate synthase. To understand mechanism of ubiquinone biosynthesis in S. pombe, we have cloned gene encoding synthase combination PCR amplification fragment and subsequent library screening. The determined DNA sequence gene, called dps, revealed that dps encodes a 378-amino-acid protein has typical conserved regions observed many polyprenyl synthases....

The nucleotide sequences of fic-1 involved in the cell filamentation induced by cyclic AMP Escherichia coli and its normal counterpart fic were analyzed. open reading frame both coded for 200 amino acids. Gly at position 55 Fic protein was changed to Arg Fic-1 protein. promoter activity confirmed fusing lacZ. gene downstream from found be pabA (p-aminobenzoate). There is an (ORF190) coding 190 acids upstream gene. Computer-assisted analysis showed that has sequence similarity with part CDC28...