The primary structure of a fatty‐acid‐binding protein (FABP) isolated from the liver nurse shark (Ginglymostoma cirratum) was determined by high‐performance tandem mass spectrometry (employing multichannel array detection) and Edman degradation. Shark FABP consists 132 amino acids with an acetylated N‐terminal valine. chemical molecular intact electrospray ionization ( M r = 15124 ± 2.5) in good agreement that calculated acid sequence 15121.3). displays significantly greater similarity to...

A mollusk-specific toxin, TxVIIA, having potent paralytic activity was isolated from the venom of sea snail Conus textile (Fainzilber M et al., 1991, Eur J Biochem 202:589-595). The structure reported above based upon amino acid analysis and Edman degradation. We have recently reinvestigated this toxin employing some most novel techniques in mass spectrometry. now report a revised primarily on high-energy collision-induced dissociation two Asp17-N peptides reduced, pyridinylethyl derivative...

C2H2 zinc finger proteins (ZFPs) play important roles in plant development and response to abiotic stresses, have been studied extensively. However, there are few studies on ZFPs mangroves mangrove associates, which represent a unique community with robust stress tolerance. MpZFP1, is highly induced by salt the associate Millettia pinnata, was cloned functionally characterized this study. MpZFP1 protein contains two domains conserved QALGGH motifs targets nucleus. The heterologous expression...

The Ni(II) complex of the tripeptide NH2-glycine-glycine-histidine-COOH (GGH) mediates efficient protein−protein cross-linking in presence oxidants such as oxone and monoperoxyphthalic acid (MMPP). Here we demonstrate that GGH fused to amino terminus a protein can still support cross-linking. was expressed at ecotin, dimeric macromolecular serine protease inhibitor found periplasm Escherichia coli. In Ni(OAc)2 MMPP, GGH−ecotin is cross-linked give species has an apparent molecular mass dimer...

Abstract Prediction of antimicrobial resistance based on whole-genome sequencing data has attracted greater attention due to its rapidity and convenience. Numerous machine learning–based studies have used genetic variants predict drug in Mycobacterium tuberculosis (MTB), assuming that are homogeneous, most these studies, however, ignored the essential correlation between corresponding genes when encoding variants, a limited number as prediction input. In this study, taking advantage...

BACKGROUND Acute mastitis is a common disease occurring in postpartum lactating women. This study aimed to identify the high-risk factors for suppurative (SM) women who were breastfeeding. MATERIAL AND METHODS A total of 262 non-SM patients and 63 SM admitted Department Breast Thyroid Surgery Liaocheng People's Hospital retrospectively enrolled this study. The during lactation determined by assessing admission time after acute mastitis, galactostasis, primipara or pluripara, nipple injuries,...

An undescribed cytotoxic cyclopeptide named phomopamide A (1) was isolated from Diaporthe sp., which is an endophytic fungus Artemisia argyi. Phomopamide featured pentadepsipeptide skeleton and composed of two Phe, one Val, Leu, 2-hydroxyoctanoic acid units. The structure this new compound fully characterised on the basis extensive spectroscopic analysis. Moreover, evaluated for in vitro cyctotoxic α-glucosidase inhibitory activity. As a result, phomopaminde exhibited no activity against...

The primary structure of rat heart muscle fatty acid-binding protein was investigated by liquid secondary ion mass spectrometry. digested with trypsin, chymotrypsin, and Staphylococcus aureus V8 protease the resulting peptides were separated reverse phase high performance chromatography. masses protonated molecular ions (MH+) tryptic, chymotryptic, S. determined spectrometry analysis using 20-500 pmol material. From tryptic digest, two MH+ 1036 861 initially found that did not match...

The irreversible inhibition of human immunodeficiency virus type 1 (HIV-1) and 2 (HIV-2) proteases by 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) eight haloperidol derivatives has been studied. EPNP specifically inhibits HIV-1 HIV-2 with a stoichiometry one molecule/dimeric enzyme. site modification protease unambiguously identified as Asp-25 using high performance tandem mass spectrometry. assayed consist epoxides, ynones, alpha,beta-unsaturated ketones. Kinact values for these range from...

Irreversible inhibition of the HIV-1 protease by agents that specifically alkylate its catalytic aspartate residues is a potentially useful approach for circumventing evolution HIV strains are resistant to inhibitors. Five haloperidol- and two FMOC-based epoxides differing reactivities have been synthesized tested as irreversible inhibitors (HIV-1 PR). Of these, trisubstituted epoxides, cis-1,2-disubstituted epoxide, 1,1-disubstituted monosubstituted epoxide function inhibitors, but...

Abstract Diaspirin crosslinked hemoglobin (DCLHb) was analyzed by mass spectrometric‐based techniques to identify the protein modifications effected crosslinking reaction with bis(3,5‐dibromosalicyl) fumarate. DCLHb consists of two principal components. These components were isolated size‐exclusion chromatography and identified measurement their molecular weight using electrospray spectrometry subsequent peptide mapping spectrometric sequence analysis individual digests. Three major RP‐HPLC...

Yak milk, a high-quality is one of the best raw materials for dairy products and economically important to pastoral herdsmen. To make further understanding molecular differences in mammary tissues yaks with different milk production during lactation, this study, we took use RNA-seq perform high-throughput sequencing analysis gland transcriptomes both high-yielding yak low-yielding lactation. By comparison transcriptome data tissue low-yield yak, 144 differential genes were screened out,...

Foeniculins A-C (1-3) together with a pair of enantiomers (±)-foeniculin D (4) were isolated from endophytic fungus Diaporthe foeniculina BZM-15. Their structures including absolute configurations unambiguously established by extensive interpretation the NMR and HR-ESI-MS data, ECD measurements powered molecular calculations, as well Mo2(OAc)4 mediated CD methodology. The cytotoxic activity assay disclosed that these compounds didn't show any noticeable activity.[Formula: see text].

Zeolite BEA with microporous and mesoporous structure was prepared through alkali etching of traditional zeolite BEA. The changes crystal form, pore morphology before after treatment its purification effect on ink wastewater were studied. effects amount, time temperature COD chroma removal rate are systematically results show that the alkali-etching-modified maintains zeolite, has higher specific surface area, volume size distribution. Under optimal conditions, performance than rates can...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnzymatic synthesis of phosphotyrosine-containing peptides via adenylated intermediatesBradford W. Gibson, Wade Hines, Zhonghua Yu, George L. Kenyon, Linda McNemar, and Joseph J. VillafrancaCite this: Am. Chem. Soc. 1990, 112, 23, 8523–8528Publication Date (Print):November 1, 1990Publication History Published online1 May 2002Published inissue 1 November 1990https://doi.org/10.1021/ja00179a042Request reuse permissionsArticle...

Abstract The paper studied about the characteristics of ink sludge flexo printing and analyzed possibility different reduction treatment disposal. Industrial analysis elemental to confirm content, TGA DTG were used analyze weight loss heat absorption desorption behavior dry at temperature. GC-MS XRF was applied composition content compounds. Three kinds for based on needs.

Background and objective: The area of mangroves is gradually decreasing globally, are already one the most threatened ecosystems. Despite net growth in mangrove areas China, restoration potential still insufficient. This study proposed Random forest model as an excellent data mining method to map based on predicted probability habitat suitability.Methods: We demonstrated vital environmental variables influencing suitability. de-cisive advantages RFM were parsimonious (variables selection),...

Abstract N ‐Trifluoroacetyl‐ n ‐butyl ester derivatives of deuterium‐labelled valine, leucine, isoleucine and phenylulanine were analysed by the isotope dilution technique gas chromatography/mass spectrometry (GC/MS). Selected ion retrieval peak‐area integration applied. The conditions adopted gave good separations with a short experimental time high intensity. Ratios total isotopic abundance metabolic kinetic curves obtained. regression coefficient standard curve was 0.99 relative deviation...