A5090933947- CRISPR and Genetic Engineering
- Dental Education, Practice, Research
- Advanced biosensing and bioanalysis techniques
- Cardiomyopathy and Myosin Studies
- RNA Interference and Gene Delivery
- Dental Health and Care Utilization
- Healthcare Policy and Management
- Advanced Electron Microscopy Techniques and Applications
- Periodontal Regeneration and Treatments
- Muscle Physiology and Disorders
- Primary Care and Health Outcomes
- Public Health Policies and Education
- Elasticity and Material Modeling
- Global Health Workforce Issues
- Cellular Mechanics and Interactions
- Connective tissue disorders research
- Laser-induced spectroscopy and plasma
- dental development and anomalies
- Cardiovascular Effects of Exercise
- Dental Implant Techniques and Outcomes
- Biotin and Related Studies
- Force Microscopy Techniques and Applications
- Metal and Thin Film Mechanics
- Ion-surface interactions and analysis
- Nanopore and Nanochannel Transport Studies
Medical Research Council
2022-2024
Imperial College London
2021-2024
MRC London Institute of Medical Sciences
2021-2023
University of Kent
2021
The University of Texas at Austin
2013
Daytona State College
1987
United States Public Health Service
1953-1969
CRISPR-Cas9 is a powerful gene-editing technology; however, off-target activity remains an important consideration for therapeutic applications. We have previously shown that force-stretching DNA induces and hypothesized distortions of the topology in vivo, such as negative supercoiling, could reduce Cas9 specificity. Using single-molecule optical-tweezers, we demonstrate supercoiling λ-DNA sequence-specific binding at multiple sites, even low forces. adapted CIRCLE-seq approach, detect over...
CRISPR-Cas12a target search and cleavage on force-stretched λ -DNA using optical tweezers.
While many studies have examined the effects mechanical forces on vSMCs, there is a limited understanding of how different arterial strain waveforms that occur in disease and vascular beds alter vSMC mechanotransduction phenotype. Here, we present novel system for applying complex, time-varying to cultured cells use this understand these can phenotype signaling. We developed highly adaptable cell culture allows application reproduce complex dynamic environment experienced by body. Using...
The Growing Shortage of Dentists in the United States Quentin M. Smith CopyRight https://doi.org/10.2105/AJPH.48.1.38 Published Online: August 29, 2011
WITH the surge of interest in prepayment dental care plans, a concomitant has developed predicting probable future de¬ mands for services by consumer groups under different arrangements providing care.This arises from necessity establishing premiums which are sufficiently great to sustain program and at same time low enough attract prospective sub¬ scribers.However, data indicating extent levels or character service demands may be predicted generally lacking.This report is third series based...
Regulated thin filaments (RTFs) tightly control striated muscle contraction through calcium binding to troponin, which enables tropomyosin expose myosin-binding sites on actin. Myosin holds in an open position, exposing more actin, leading cooperative activation. At lower levels, troponin and turn off the filament; however, this is antagonised by high local concentration of myosin, questioning how filament relaxes. To provide molecular details deactivation, we used single-molecule imaging...
Dr. Smith presents briefly and concisely, as usual, some convincing demands on the public dentist to accomplish in Comprehensive Health Planning.
IN 1949 the House of Delegates Ameri¬ can Dental Association, recognizing need for "a more satisfactory financing plan to meet dental health service costs, especially medium and low income groups" (i), endorsed experimentation with voluntary prepaid care programs by societies.Such experi¬ ments would prove whether prepayment principle which had been so successfully applied hospital medical costs be equally workable dentistry.Even in 1949, plans were not new, some having predated World War...
CRISPR/Cas9 is a powerful gene-editing technology, however off-target activity remains an important consideration for therapeutic applications. We have previously shown that force stretching DNA induces and hypothesised distortions of the topology in vivo, such as negative supercoiling, could reduce Cas9 specificity. Using single-molecule optical-tweezers, we demonstrate supercoiling λ-DNA sequence-specific binding at multiple sites, even low forces. By using adapted CIRCLE-seq approach,...
Report of the Committee on Health Manpower, 1970 Affiliation Quentin M. Smith, Ralph H. Boatman, Roy J. Boston, Joseph A. Gallagher, C. Graber, Hines, William McC. Hiscock, Marion Murphy, Donald F. Simpson, and Robert G. Webster CopyRight https://doi.org/10.2105/AJPH.61.3.612 Published Online: August 29, 2011