A5011673957- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- Advanced MRI Techniques and Applications
- Solid-state spectroscopy and crystallography
- Electron Spin Resonance Studies
- Atomic and Subatomic Physics Research
- Muon and positron interactions and applications
- Protein Structure and Dynamics
- Neuroscience and Neuropharmacology Research
- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Influenza Virus Research Studies
- Metallurgy and Material Forming
- Cholinesterase and Neurodegenerative Diseases
- Synthesis and Properties of Aromatic Compounds
- RNA and protein synthesis mechanisms
- Advanced Neuroimaging Techniques and Applications
- Ionic liquids properties and applications
- Antifungal resistance and susceptibility
- Advanced Thermodynamic Systems and Engines
- Field-Flow Fractionation Techniques
- Signaling Pathways in Disease
- Building Energy and Comfort Optimization
- Microfluidic and Capillary Electrophoresis Applications
- Magnetic properties of thin films
Max Planck Institute for the Study of Religious and Ethnic Diversity
2022-2025
Max Planck Institute for Multidisciplinary Sciences
2022-2025
Tissue Dynamics (Israel)
2024
Max Planck Institute for Biophysical Chemistry
2020-2023
University of Illinois Urbana-Champaign
2019-2023
New York University
2016-2019
Urbana University
2019
University of Illinois System
2019
Tel Aviv University
2010-2018
A passive cooling strategy without any electricity input has shown a significant impact on overall energy consumption globally. However, designing tunable daytime radiative cooler to meet requirement of different weather conditions is still big challenge, especially in hot, humid regions. Here, novel type tunable, thermally insulating and compressible cellulose nanocrystal (CNC) aerogel coolers prepared via chemical cross-linking unidirectional freeze casting process. Such can achieve...
Aggregation intermediates play a pivotal role in the assembly of amyloid fibrils, which are central to pathogenesis neurodegenerative diseases. The structures filamentous and mature fibrils now efficiently determined by single-particle cryo-electron microscopy. By contrast, smaller pre-fibrillar α-Synuclein (αS) oligomers, crucial for initiating amyloidogenesis, remain largely uncharacterized. We report an atomic-resolution structural characterization toxic aggregation intermediate (I1) on...
The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency 1 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected improve resolution, provided sample preparation results homogeneous broadening. We compare two-dimensional (2D) detected MAS spectra of four membrane proteins at 950 and 1200 MHz. find a consistent improvement that scales superlinearly with...
Aggregation of amyloidogenic proteins is a characteristic multiple neurodegenerative diseases. Atomic resolution small molecule binding to such pathological protein aggregates interest for the development therapeutics and diagnostics. Here we investigate interaction between α-synuclein fibrils anle138b, clinical drug candidate disease modifying therapy in neurodegeneration promising scaffold positron emission tomography tracer design. We used nuclear magnetic resonance spectroscopy cryogenic...
The currently circulating S31N variant of the M2 proton channel influenza A is resistant to antiviral drugs. Recently, there has been a growing concern regarding impact lipid environment on structural features variant. native symmetry tetramer remains controversial. Here we show that persists in dimer-of-dimers structurein different preparations independent amount solvating lipids up at least 180 per tetramer. Two isoleucine residues with upfield shifted alpha carbon resonances, which are...
Homonuclear dipolar recoupling is routinely used for magic-angle spinning NMR-based structure determination. In fully protonated samples, only short proton-proton distances are accessible to broadband approaches because of high proton density. Selective methods allow detection longer by directing polarization a subset spins. Here we introduce the selective pulse sequence MODIST, which recouples spins that have modest chemical shift offset difference, and demonstrate it selectively record...
Metal ions, including biologically prevalent sodium can modulate electrostatic interactions frequently involved in the stability of condensed compartments cells. Quantitative characterization heterogeneous ion dynamics inside biomolecular condensates demands new experimental approaches. Here we develop a 23Na NMR relaxation-based integrative approach to probe ions agarose gels as model system. We exploit electric quadrupole moment spin-3/2 nuclei and, through combination single-quantum and...
Recently developed homonuclear transverse mixing optimal control pulses (hTROP) revealed an elegant way to enhance the detected signal in multidimensional magic-angle spinning (MAS) nuclear magnetic resonance experiments. Inspired by their work, we present two simplified preservation of equivalent pathways spectroscopy (hSPEPS) sequences for recoupling CA-CO and CA-CB dipolar couplings under fast ultrafast MAS rates, theoretically enabling a √2 improvement sensitivity each indirect...
Inspired by the recently proposed transverse mixing optimal control pulses (TROP) approach for improving signal in multidimensional magic-angle spinning (MAS) NMR experiments, we present simplified preservation of equivalent pathways spectroscopy (SPEPS). It transfers both components magnetization that occur during indirect evolutions, theoretically enabling a √2 improvement sensitivity each such dimension. We compare SPEPS transfer with TROP and cross-polarization (CP) using membrane...
Fast-magic-angle-spinning solid-state NMR is a developing technique for determination of protein structure and dynamics. Proton-proton correlations usually lead to rough distance restraints, serious hurdle towards high-resolution structures. Analogous the "eNOE" concept in solution, an integrative approach more accurate restraints enables improved structural accuracy with minimal analytical effort.
Recently developed homonuclear Transverse mixing Optimal control Pulses (hTROP) revealed an elegant way to enhance the detected signal in multi-dimensional magic-angle spinning (MAS) NMR experiments. Inspired by their work, we present two simplified preservation of equivalent pathways spectroscopy (hSPEPS) sequences for recoupling CA-CO and CA-CB dipolar couplings under fast ultra-fast MAS rates, theoretically enabling a √2 improvement sensitivity each indirect dimension. The efficiencies...
Abstract Magic‐angle spinning solid‐state NMR spectroscopy has been applied to study the dynamics of CBM3b–Cbh9A from Clostridium thermocellum ( ct CBM3b), a cellulose binding module protein. This 146‐residue protein nine‐stranded β‐sandwich fold, in which 35 % residues are β‐sheet and remainder composed loops turns. Dynamically averaged 1 H‐ 13 C dipolar coupling order parameters were extracted site‐specific manner by using pseudo‐three‐dimensional constant‐time recoupled...
Small molecules that bind to oligomeric protein species such as membrane proteins and fibrils are of clinical interest for development therapeutics diagnostics. Definition the binding site at atomic resolution via NMR is often challenging due low stoichiometry small molecule. For aggregation intermediates grown in presence lipids, we report atomic-resolution contacts molecule sub nm distance solid-state using dynamic nuclear polarization (DNP) orthogonally labelled samples We apply this...
Internuclear distance determination is the foundation for NMR-based structure calculation. However, high-precision measurement a laborious process requiring lengthy data acquisitions due to large set of multidimensional spectra needed at different mixing times. This prevents application or challenging molecular systems. Here, we present new approach, transferred-rotational-echo double resonance (TREDOR), heteronuclear transfer method in which simultaneously detect both starting and...
Cross polarization (CP) transfers via Hartmann-Hahn matching conditions are one of the cornerstones solid-state magic-angle spinning NMR experiments. Here we investigate a windowed sequence for cross (wCP) at 55 kHz spinning, placing window (and pulse) per rotor period on or both rf channels. The wCP is known to have additional conditions. We observe striking similarity between and CP transfer when considering flip angle pulse rather than rf-field strength applied during pulse. Using...
The rotational echo adiabatic passage double resonance (REAPDOR) magic angle spinning NMR experiment efficiently recouples the dipolar interaction between a spin-1/2 and spin >1/2, enabling accurate efficient measurement of their inter-nuclear distance. We demonstrate that under conditions universal curve depends only on distance fits REAPDOR recoupling for (13)C (spin-1/2)-(11)B (spin-3/2) pair. In 4-(hydroxymethyl)phenylboronic acid MIDA ester methyl carbon boron was determined to be 2.76...
The distance between a spin one-half and an attached possessing large anisotropy can be obtained using different dipolar recoupling sequences that are based on the rotational-echo double resonance technique under magic-angle spinning solid-state NMR. general difference these with respect to coupled is set of pulses applied in order drive this out equilibrium, thereby interaction. Since complete inversion practically not possible due coupled-spin anisotropy, one or another pulse depends...
Carbon–carbon dipolar recoupling sequences are frequently used building blocks in routine magic-angle spinning NMR experiments. While broadband homonuclear first-order mainly excite intra-residue correlations, selective methods can detect inter-residue transfers and long-range correlations. Here, we present the great offset difference internuclear transfer (GODIST) pulse sequence optimized for carbonyl or aliphatic at fast spinning, here, 55 kHz. We observe a 3- to 5-fold increase...