Citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as Huanglongbing. The bacteria, Candidatus Liberibacter asiaticus (CLas), a persistent propagative pathogen transmitted by the Asian psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Hemipterans characterized date lack number of insect immune genes, including those associated with Imd pathway targeting Gram-negative bacteria. D. draft genome was used characterize defense...

and

Citrus production worldwide is currently threatened by Huanglongbing, or citrus greening disease. The associated pathogen, Candidatus Liberibacter asiaticus (CLas), transmitted the Asian psyllid, Diaphorina citri. Annotation of D. citri genome revealed a reduced innate immune system lacking number antimicrobial peptides and Imd pathway with defense against Gram-negative bacteria. We characterized this apparent reduction in survival assays which were exposed to Gram-positive experienced...

Tetracycline repressible (Tet-Off) male-only sexing systems have great potential for improving the efficacy of sterile insect control programs in addition to reducing rearing cost. The relationship between insects and their microbial symbionts, as well physiological stress caused by tetracycline class antibiotics, pose concerns that Tet-Off strains could reduced fitness nature. Here we evaluated biological performance, midgut microbiome, transcriptomes wild-type screwworm, Cochliomyia...

The New World screwworm, Cochliomyia hominivorax, was eradicated from North and Central America through the first application of sterile insect technique. screwworm adult fly releases were mixed sex, but experience with other flies suggests that releasing only males could be up to five times more effective. Here we describe transgenic sexing strains (TSSs) expected embryo lethality developed using Tetracycline-off (Tet-off) system Lucilia cuprina nullo (DR6) or C. macellaria CG14427 (DR7)...

The potato psyllid (Bactericera cockerelli Sulc) is an economically important insect pest of solanaceous crops such as potato, tomato, pepper, and tobacco. Historically, the psyllid's range included central United States, Mexico, California; more recently, populations this have been reported in Central America, Pacific Northwest, New Zealand. Like most phytophagous insects, psyllids require symbiotic bacteria to compensate for nutritional deficiencies their diet. Potato harbor primary...

The potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae), is a pest of potato, Solanum tuberosum L., and other solanaceous crops in North Central America. This insect transmits Candidatus Liberibacter solanacearum, the putative causal agent zebra chip disease potato. Recent studies identified three genetically distinct haplotypes B. United States, correlating to geographical regions: Central, Northwestern, Western. Although biological differences among haplotypes, including...

Abstract Background The New World Screwworm fly (NWS), Cochliomyia hominivorax , is an ectoparasite of warm-blooded animals and a major pest livestock in parts South America the Caribbean where it remains endemic. In North Central was eradicated using Sterile Insect Technique (SIT). A control program managed cooperatively between governments United States Panama to prevent northward spread NWS from infested countries America. This accomplished by maintaining permanent barrier through release...

ABSTRACT Bacterial communities associated with eukaryotes play important roles in the physiology, development and health of their hosts. Additionally, composition microbes can be indicative evolutionary history, environment life history. Here, we investigated microbial ecology two sympatric caenogastropod taxa belonging to family Hydrobiidae, Juturnia kosteri Pyrgulopsis roswellensis. The diversity microbiota were described based on high-throughput Illumina sequencing targeting V4 region 16S...

Abstract Background Feral swine ( Sus scrofa ) are highly invasive and threaten animal human health in the Americas. The screwworm Cochliomyia hominivorax is listed by World Organization for Animal Health as a notifiable infestation because myiasis cases affect livestock, wildlife, humans endemic areas, outbreaks can have major socioeconomic consequences regions where has been eradicated. However, knowledge gap exists on of feral South America, endemic. Here, we report harvested Artigas...

The potato psyllid, Bactericera cockerelli (Sulc), is a native of North America and occurs from Central to Canada. A newly emerging plant pathogen, Candidatus Liberibacter solanacearum, associated with psyllids in New Zealand, has become major concern many solanaceous crops worldwide. In potato, Solanum tuberosum L., the resulting disease called zebra chip, but similar foliar vascular symptoms have been reported tomato, lycopersicum peppers, Capsicum annuum L. To date, it known that transmit...

The evolution of obligate ectoparasitism in blowflies (Diptera: Calliphoridae) has intrigued scientists for over a century, and surprisingly, the genetics underlying this lifestyle remain largely unknown. Blowflies use odors to locate food oviposition sites; therefore, olfaction might have played central role niche specialization within group. In insects, coreceptor Orco is required partner all odorant receptors (ORs), major gene family involved olfactory-evoked behaviors. Hence, we...

In September 2011, potato (Solanum tuberosum) tubers grown in Nicaragua outside of Estelí and Jinotega were observed with internal discoloration suggestive zebra chip (ZC); the plants showed foliar symptoms chlorosis, leaf scorching, wilting, vascular discoloration, swollen nodes, twisted stems, aerial (3). Disease incidence ranged from 50 to 95% eight fields ranging 5 12 ha regions Nicaragua. Leaf samples psyllids collected two fields, total DNA was purified leaves 17 symptomatic 10...

Journal Article Low-Level Detection of Candidatus Liberibacter Solanacearum in Bactericera cockerelli (Hemiptera: Triozidae) by 16s rRna Pyrosequencing Get access Alex P. Arp, Arp 2 1Department Biology, University Texas at Tyler, 3900 Blvd., TX 75799. 2Corresponding author, e-mail: aalex@patriots.uttyler.edu. Search for other works this author on: Oxford Academic PubMed Google Scholar Rebekah Chapman, Chapman James M. Crosslin, Crosslin 3Vegetable and Forage Crops Research Unit, USDA-ARS,...

Abstract The New World screwworm, Cochliomyia hominivorax (Coquerel 1858) (Diptera: Calliphoridae), is a serious parasite of livestock, humans, and other warm-blooded animals. It has been eradicated from the northern parts its historical range down to Panama—Colombian border where permanent barrier zone maintained. This eradication was accomplished through using sterile insect technique (SIT). In 2016 there an outbreak C. in Florida Keys. only six months, this pest successfully re-eradicated...

The screwworm fly, Cochliomyia hominivorax (Coquerel), was successfully eradicated from the United States by sterile insect technique (SIT). However, recent detection of these flies in Florida Keys, and increased risk introductions to other areas warrant novel tools for management flies. Surveillance, a key component control programs, utilizes traps baited with rotting liver or blend synthetic chemicals such as swormlure-4. In this work, we evaluated olfactory physiology fly compared it...

Abstract New World screwworm flies, Cochliomyia hominivorax (Coquerel), are obligate parasites of warm-blooded animals. They were eradicated from North and Central America during the mid-20th to early-21st centuries using sterile insect technique (SIT), a method presently employed maintain permanent barrier between South America. Lures an important component eradication program, where they used for surveillance, sample collection, strain evaluation in field. The first chemical lure, later...

Abstract Transgenic insect strains with tetracycline repressible (Tet-Off) female-lethal genes provide significant advantages over traditional sterile techniques for population control, such as reduced diet and labor costs more efficient suppression. Tet-Off systems are suppressed by tetracycline-class antibiotics, most commonly (Tc) or doxycycline (Dox), allowing equal sex ratio colonies of transgenic insects when reared Tc Dox male-only generations in their absence. is a stable molecule...

Abstract Insect population control through continual releases of large numbers sterile insects, called insect technique (SIT), is only possible if one can mass-rear quantities healthy insects. Adaptation stocks to rearing conditions and artificial feeding systems have a multitude negative effects such as inbreeding depression, reduced compatibility with wild strains, unintentional selection for traits that lower fitness after release, an altered microbiome. Changes microbiomes many on...

Abstract Screwworm flies are mass-reared and released along the Panama-Colombia border to prevent reinfestation of Central North America. The cost production facility, labor, diet materials makes mass-rearing most expensive component program. has a large impact on quality quantity insects produced, both which necessary for successful implementation sterile insect technique. currently used rear screwworm in Panama contains dried bovine red blood cells, plasma, egg powder, milk replacement...

Abstract Background: Feral swine ( Sus scrofa ) are highly invasive and threaten animal public health in the Americas. Screwworm Cochliomyia hominivorax is listed by World Organization for Animal Health as a notifiable infestation because myasis cases affect livestock, wildlife, humans endemic areas, outbreaks can be of high socioeconomic consequence regions where screwworm was eradicated. However, knowledge gap exists on feral South America, endemic. Here, we report with harvested...

Abstract BackgroundFeral swine ( Sus scrofa ) are highly invasive and threaten animal public health in the Americas. New World screwworm Cochliomyia hominivorax is listed by Organization for Animal Health as a notifiable infestation because myasis cases affect livestock, wildlife, humans endemic areas, outbreaks can be of high socioeconomic consequence regions where was eradicated. However, knowledge gap exists on feral South America C . endemic. Here, we report with harvested Department...