A5001154407- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- RNA modifications and cancer
- Blood groups and transfusion
- CRISPR and Genetic Engineering
- Antifungal resistance and susceptibility
- RNA regulation and disease
- Bacterial biofilms and quorum sensing
- Genomics and Chromatin Dynamics
- Inflammasome and immune disorders
- Kruppel-like factors research
- Pancreatic function and diabetes
- RNA Research and Splicing
- PARP inhibition in cancer therapy
- Cancer, Hypoxia, and Metabolism
- Parvovirus B19 Infection Studies
- Skin and Cellular Biology Research
- Zebrafish Biomedical Research Applications
- Oral microbiology and periodontitis research
University of Bristol
2014-2025
University Of Bristol Dental Hospital
2014
Candida albicans is a pleiomorphic fungus that forms mixed species biofilms with Streptococcus gordonii, an early colonizer of oral cavity surfaces. Activation quorum sensing (QS; intercellular signalling) promotes monospecies biofilm development by these micro-organisms, but the role QS in communities not understood. The comCDE genes S. gordonii encode sensor-regulator system (ComDE), which activated comC gene product (CSP, competence stimulating peptide) and modulates expression...
Report26 April 2018Open Access Transparent process Enhancement of red blood cell transfusion compatibility using CRISPR-mediated erythroblast gene editing Joseph Hawksworth School Biochemistry, University Bristol, UK Bristol Institute for Transfusion Sciences, National Health Service Blood and Transplant (NHSBT), Search more papers by this author Timothy J Satchwell NIHR Research Unit, Marjolein Meinders Deborah E Daniels Fiona Regan Imperial College Healthcare NHS Trust, London, &...
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Abstract Within the bone marrow microenvironment, endothelial cells (EC) exert important functions. Arterial EC support hematopoiesis while H-type capillaries induce formation. Here, we show that BM sinusoidal (BM-SEC) actively control erythropoiesis. Mice with stabilized β-catenin in BM-SEC ( Ctnnb1 OE-SEC ) generated by using a BM-SEC-restricted Cre mouse line Stab2-iCreF3 develop fatal anemia. While activation of Wnt-signaling causes an increase erythroblast subsets (PII–PIV), mature...
Pluripotent stem cells are attractive progenitor for the generation of erythroid in vitro as have expansive proliferative potential. However, although embryonic (ESC) and induced pluripotent (iPSC) can be to undergo differentiation, majority fail enucleate molecular basis this defect is unknown. One protein that has been associated with initial phase cell enucleation intermediate filament vimentin, loss vimentin potentially required process proceed. In study, we used our established culture...
Abstract Erythropoiesis requires a combination of ubiquitous and tissue-specific transcription factors (TFs). Here, through DNA affinity purification followed by mass spectrometry, we have identified the widely expressed protein MAZ (Myc-associated zinc finger) as TF that binds to promoter erythroid-specific human α-globin gene. Genome-wide mapping in primary erythroid cells revealed also occupies active promoters well GATA1-bound enhancer elements key genes. Consistent with an important...
Abstract β-thalassemia is a prevalent genetic disorder causing severe anemia due to defective erythropoiesis, with few treatment options. Studying the underlying molecular defects impeded by paucity of suitable patient material. In this study we create human disease cellular model systems for gene editing erythroid line BEL-A, which accurately recapitulate phenotype cells. We also develop high throughput compatible fluorometric-based assay evaluating severity and utilize demonstrate that...
Human ZNF648 is a novel poly C-terminal C2H2 zinc finger (ZnF) protein identified amongst the most dysregulated proteins in erythroid cells differentiated from induced pluripotent stem cells. Its nuclear localization and structure indicate it likely DNA-binding protein. Using combination of overexpression an line primary adult cells, knockdown megakaryocytes, comparative proteomics transcriptomics we show that required for both megakaryocyte differentiation. Orthologues were detected across...
2020 of Haematologica, 1 during the final processing manuscript labeling Figure 2E got accidentally inverted.On both BEL-A and HUDEP-2 graphs, γ β globin labels are wrong way around.The corrected panel E 2 is shown here.
Abstract β-thalassemia is a prevalent genetic disorder causing severe anemia due to defective erythropoiesis, with few treatment options. Studying the underlying molecular defects impeded by paucity of suitable patient material. In this study we created human disease cellular model systems for β-thalassemia, which accurately recapitulate phenotype erythroid cells. We also developed high throughput compatible fluorometric-based assay evaluating severity and utilised demonstrate positive...