A5076386746- Cutaneous lymphoproliferative disorders research
- Lymphoma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Autoimmune Bullous Skin Diseases
- Eosinophilic Disorders and Syndromes
- Immunotherapy and Immune Responses
- Genetic and rare skin diseases.
- Dermatology and Skin Diseases
- Nail Diseases and Treatments
- Immune cells in cancer
- Histiocytic Disorders and Treatments
- T-cell and Retrovirus Studies
- Fungal Infections and Studies
- Immune Cell Function and Interaction
- Cancer and Skin Lesions
- Liver Disease Diagnosis and Treatment
- Vascular Tumors and Angiosarcomas
- Angiogenesis and VEGF in Cancer
- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Dermatological and Skeletal Disorders
- Liver physiology and pathology
- Blood disorders and treatments
- Psoriasis: Treatment and Pathogenesis
- Autoimmune and Inflammatory Disorders
University Hospital Heidelberg
2016-2025
Heidelberg University
2016-2025
University Medical Centre Mannheim
2016-2025
Oncoscience (Germany)
2024-2025
University Medical Center
2002-2022
LMU Klinikum
2020
Ludwig-Maximilians-Universität München
2005-2020
Eastern Cooperative Oncology Group
2020
Medizinische Fakultät Mannheim
2011-2019
University of Mannheim
2006-2019
Angiopoietin-2 (ANG-2) is a key regulator of angiogenesis that exerts context-dependent effects on ECs. ANG-2 binds the endothelial-specific receptor tyrosine kinase 2 (TIE2) and acts as negative ANG-1/TIE2 signaling during angiogenesis, thereby controlling responsiveness ECs to exogenous cytokines. Recent data from tumors indicate under certain conditions can also promote angiogenesis. However, molecular mechanisms dual functions are poorly understood. Here, we identify model for opposing...
Alternative activation of macrophages, induced by Th 2 cytokines and glucocorticoids, is essential for the proper functioning anti‐inflammatory immune reactions. To this end, alternatively activated macrophages (aaMΦ) express a not yet fully unravelled set genes including such as alternative macrophage activation‐associated CC‐chemokine (AMAC)‐1 pattern recognition molecules scavenger receptor CD163. In order to further characterize molecular repertoire aaMΦ, differential gene expression was...
MS-1, a high-molecular-mass protein expressed by non-continuous and angiogenic endothelial cells alternatively activated macrophages (Mφ2), the hepatic sinusoidal hyaluronan clearance receptor are similar with respect to tissue distribution biochemical characteristics. In present study we purified these proteins immuno- hyaluronan-affinity chromatography respectively, sequenced tryptic peptides generated full-length cDNA sequences in both mouse human. The novel genes, i.e. stabilin-1...
Lymphangiogenesis is a novel prognostic parameter for several cancers that preferentially quantified by immunohistochemistry of the lymphatic endothelium-specific hyaluronan receptor LYVE-1. Recently, specificity LYVE-1 was challenged serendipitous observations expression in rare tissue macrophages. As receptor-like molecule stabilin-1 shared sinusoidal endothelium and macrophages, thorough analysis performed using macrophage-specific markers vivo vitro. In murine tumour models excisional...
Abstract We have cloned a novel human CC-chemokine, alternative macrophage activation-associated CC-chemokine (AMAC)-1. The isolated cDNA clone (803 bp) shows single open reading frame of 267-bp coding for 89 amino acid residues; mature AMAC-1 protein is predicted to consist 69 acids with m.w. 7855. Sequence alignment and 3D-modeling show the typical structural characteristics CC-chemokines special features in receptor-activating domain. most closely related MIP-1α sequence homology 55% 59%,...
MS-1, a high-molecular-mass protein expressed by non-continuous and angiogenic endothelial cells alternatively activated macrophages (Mφ2), the hepatic sinusoidal hyaluronan clearance receptor are similar with respect to tissue distribution biochemical characteristics. In present study we purified these proteins immuno- hyaluronan-affinity chromatography respectively, sequenced tryptic peptides generated full-length cDNA sequences in both mouse human. The novel genes, i.e. stabilin-1...
Abstract The matricellular protein SPARC (secreted acidic and rich in cysteine) has been implicated development, differentiation, response to injury, tumor biology by virtue of its regulation extracellular matrix production/assembly antiadhesive antiproliferative effects on different cell types. Despite numerous biological activities described for SPARC, surface receptors this have not identified. By phage display vitro-binding assays, we now show that interacts with stabilin-1, a scavenger...
Human Glyco_18 domain-containing proteins constitute a family of chitinases and chitinase-like proteins. Chitotriosidase AMCase are true enzymes which hydrolyse chitin have C-terminal chitin-binding domain. YKL-40, YKL-39, SI-CLP murine YM1/2 possess solely domain do not the hydrolytic activity. The major sources containing macrophages, neutrophils, epithelial cells, chondrocytes, synovial cancer cells. Both macrophages neutrophils use regulated secretory mechanism for release established...
Tumor-associated macrophages (TAMs) constitute major infiltrates of solid tumors and express a marker profile that characterizes alternatively activated (MФs). TAMs accumulate in hypoxic tumor regions, high amounts hypoxia-inducible factor-1 (HIF-1) contribute to angiogenesis invasiveness. However, the precise role HIF-1 on MФ infiltration phenotype alterations remains poorly defined. Therefore, we cocultured wild type (wt) versus HIF-1α −/− MФs with spheroids. Both, wt MФs, infiltrated...
Tissue homeostasis and remodeling are processes that involve high turnover of biological macromolecules. Many the waste molecules by-products or degradation intermediates macromolecule enter circulation subsequently cleared by liver sinusoidal endothelial cells (LSEC). Besides mannose receptor, stabilin-1 stabilin-2 major scavenger receptors expressed LSEC. To more clearly elucidate functions -2, we have generated mice lacking stabilin-1, stabilin-2, both -2 (Stab1–/–Stab2–/– mice). Mice...
Microvascular endothelial cells (ECs) are increasingly recognized as organ-specific gatekeepers of their microenvironment. ECs instruct neighboring in vascular niches through angiocrine factors, which include secreted growth factors (angiokines), extracellular matrix molecules, and transmembrane proteins. However, the molecular regulators that drive microvascular transcriptional programs thereby regulate angiodiversity largely elusive. In contrast to other ECs, form a continuous cell layer,...
Angiocrine signaling by liver sinusoidal endothelial cells (LSECs) regulates hepatic functions such as growth, metabolic maturation, and regeneration. Recently, we identified GATA4 the master regulator of LSEC specification during development. Herein, studied role in adult pathogenesis.We generated Clec4g-icretg/0xGata4fl/fl (Gata4LSEC-KO) mice with LSEC-specific depletion Gata4. Livers were analyzed histology, electron microscopy, immunohistochemistry/immunofluorescence, situ hybridization,...
Abstract Unbiased dissection of T-cell receptor (TCR) repertoire diversity at the nucleotide level could provide important insights into human immunity. Here we show that TCR ligation-anchored-magnetically captured PCR (TCR-LA-MC PCR) identifies α- and β-chain without sequence-associated or quantitative restrictions in healthy diseased conditions. TCR-LA-MC convergent recombination events, classifies different stages cutaneous lymphoma vivo demonstrates reactivation after vitro...