A5040907172- Vitamin D Research Studies
- Pregnancy and preeclampsia studies
- Reproductive System and Pregnancy
- Birth, Development, and Health
- Ion channel regulation and function
- Estrogen and related hormone effects
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Gestational Diabetes Research and Management
- Preterm Birth and Chorioamnionitis
- Pregnancy and Medication Impact
- Cancer Cells and Metastasis
- RNA modifications and cancer
- Amino Acid Enzymes and Metabolism
- Natural Compound Pharmacology Studies
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- COVID-19 Impact on Reproduction
- Electrolyte and hormonal disorders
- Inflammatory mediators and NSAID effects
- Sarcoma Diagnosis and Treatment
- Synthesis of Organic Compounds
- Biochemical Acid Research Studies
- NF-κB Signaling Pathways
- Sulfur Compounds in Biology
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán
2015-2025
Instituto Nacional de Enfermedades Respiratorias
2021
Universidade Estadual Paulista (Unesp)
2016
In pregnancy, maternal serum concentrations of calcitriol significantly rise as a result increased renal and placental contribution in order to assure calcium supply for the developing fetus. Considering that placenta is site vitamin D activation, versatility potency calcitriol, it feasible this hormone participates fetal/placental development physiology. present work we studied actions upon human chorionic gonadotropin (hCG) secretion expression cultured trophoblasts, well receptor (VDR)...
Abstract Ether à go-go (EAG) potassium channels display oncogenic properties. In normal tissues, EAG mRNA is almost exclusively expressed in brain, but it several somatic cancer cell lines, including HeLa, from cervix. Antisense experiments against eag reduce proliferation some and inhibition of EAG-mediated currents has been suggested to decrease a melanoma line. Because the potential clinical relevance EAG, we investigated expression following fresh samples human uterine cervix: 5 primary...
Cervical cancer is the fourth most common among women worldwide. The main factor associated with onset and progression of this neoplasia human papillomavirus (HPV) infection. HPV-oncogenes E6 E7 are critical drivers cellular transformation, promoting expression oncogenes such as KCNH1. phytochemical α-mangostin (AM) a potent antineoplastic antiviral compound. However, its effects on HPV KCNH1 gene remain unknown. This study evaluated AM cell proliferation, cycle distribution expression,...
Abstract Ether-à-go-go-1 (Eag1) potassium channels are potential tools for detection and therapy of numerous cancers. Here, we show human Eag1 (hEag1) regulation by cancer-associated factors. We studied hEag1 gene expression its estradiol, antiestrogens, papillomavirus (HPV) oncogenes (E6/E7). Primary cultures from normal placentas cervical cancer tissues; tumor cell lines cervix, choriocarcinoma, keratinocytes, lung; vascular endothelium, lung were used. Reverse transcription-PCR (RT-PCR)...
Background Calcitriol antiproliferative effects include inhibition of the oncogenic ether-à-go-go-1 potassium channel (Eag1) expression, which is necessary for cell cycle progression and tumorigenesis. Astemizole, a new promising antineoplastic drug, targets Eag1 by blocking ion currents. Herein, we characterized interaction between calcitriol astemizole as well their conjoint action in SUM-229PE, T-47D primary tumor-derived breast cancer cells. Methodology/Principal Findings Molecular...
Calcitriol is a multitarget anticancer hormone; however, its effects on angiogenesis remain contradictory. Herein, we tested whether the antiangiogenic phytochemicals curcumin or resveratrol improved calcitriol antitumorigenic in vivo. Triple-negative breast cancer tumoral cells (MBCDF-T) were xenografted nude mice, maintaining treatments for 3 weeks. Tumor onset, volume and microvessel density significantly reduced mice coadministered with (Cal+Cur). Vessel count was also simultaneously...
The human placenta synthesizes 1,25-dihydroxyvitamin D3 and expresses the vitamin D receptor. Because preeclampsia (PE) is associated with low circulating levels of maternal IGF-I, it possible that alterations in calcium metabolism seen PE could occur at level fetoplacental unit. In this study, patterns gene expression enzyme activity 25-hydroxyvitamin d-1α-hydroxylase (1α-hydroxylase) abundance IGF-I mRNA placentas from normal (NT) PE-complicated pregnancies were investigated. Cultured...
Approximately 30% of breast tumors do not express the estrogen receptor (ER) α, which is necessary for endocrine therapy approaches. Studies are ongoing in order to restore ERα expression ERα-negative cancer. The aim present study was determine if calcitriol induces ER-negative cancer cells, thus restoring antiestrogen responses. Cultured cells derived from and an cell line (SUM-229PE) were treated with assessed by real time PCR western blots. functionality evaluated prolactin gene analysis....
The oncogenic ether-à-go-go-1 potassium channel (EAG1) activity and expression are necessary for cell cycle progression tumorigenesis. active vitamin D metabolite, calcitriol, astemizole, a promising antineoplastic drug, target EAG1 by inhibiting its blocking ion currents, respectively. We have previously shown synergistic antiproliferative effect of calcitriol astemizole in breast cancer cells vitro, but the this dual therapy vivo has not been studied.In present study, we explored combined...
Although accumulating data show that placenta is able to synthesize 1,25-dihydroxyvitamin D3, the presence of cytochrome P450 enzyme capable converting 25-hydroxyvitamin D3 (25OHD3) biologically active form vitamin D in this tissue, has not been yet clearly established. In study, we have investigated 1α-hydroxylase (1α-(OH)ase) gene expression products cultured human syncytiotrophoblast. Total RNA was isolated from placental cells and subjected Northern blots or RT-PCR by using...
The vitamin D endocrine system, primarily mediated by its main metabolite calcitriol and the receptor (VDR), plays a critical role in numerous human physiological processes, ranging from calcium metabolism to prevention of various tumors, including cervical cancer. In this study, we comprehensively investigated genomic regulatory effects cancer model. We examined transcriptional changes induced CaSki cells, cell line harboring multiple copies HPV16, primary causal agent Our microarray...
MicroRNAs play important roles in cancer biology. Calcitriol, the hormonal form of vitamin D3, regulates microRNAs expression tumor cells. In present study we asked if calcitriol would modify some components microRNA processing machinery, namely, Drosha and Dicer, calcitriol-responsive cervical We found that treatment did not affect mRNA; however, it significantly increased Dicer mRNA protein VDR-positive SiHa HeLa VDR-negative C33-A cells, had no effect on mRNA. also a D response element...