A5012948217- Helicobacter pylori-related gastroenterology studies
- RNA modifications and cancer
- Redox biology and oxidative stress
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Galectins and Cancer Biology
- Clostridium difficile and Clostridium perfringens research
- Circular RNAs in diseases
- Coenzyme Q10 studies and effects
- Insect and Pesticide Research
- Phytochemistry and biological activity of medicinal plants
- Animal Genetics and Reproduction
- Antibiotic Resistance in Bacteria
- Ginseng Biological Effects and Applications
- Reproductive tract infections research
- Chemical Reactions and Isotopes
- Silk-based biomaterials and applications
- Glutathione Transferases and Polymorphisms
- Bioinformatics and Genomic Networks
- MicroRNA in disease regulation
- Natural product bioactivities and synthesis
- Veterinary medicine and infectious diseases
Beihua University
2006-2025
Jiangsu University
2007-2011
Background The rising incidence of breast cancer and its heterogeneity necessitate precise tools for predicting patient prognosis tailoring personalized treatments. Epigenetic changes play a critical role in progression therapy responses, providing foundation prognostic model development. Methods We developed the Machine Learning-derived Model (MLEM) to identify epigenetic gene patterns cancer. Using multi-cohort transcriptomic datasets, MLEM was constructed with rigorous machine learning...
Peroxiredoxin 6 (PRDX6) is an important antioxidant enzyme, with a potential application value in the treatment of diseases caused by oxidative damage. PRDX6 and mutant (mPRDX6) were heterologously expressed using E.coli expression system purified Ni-affinity chromatography. Isoproterenol (ISO) was used to induce myocardial cell injury model animal model. After mPRDX6, proliferation activity H9C2 cells detected Cell Counting Kit-8 (CCK8) method; apoptosis evaluated flow cytometry,...
Objective: This research aimed to study the expression of PRDX6 mRNA in hepatocellular carcinoma (HCC) and its effect on prognosis HCC. Moreover, gene knockdown proliferation, migration, invasion HepG2 cells mediated by lentivirus was also examined. offers a theoretical experimental basis for further mechanism liver cancer new methods clinical diagnosis treatment. Methods: RNA sequence data 369 HCC patients were screened through TCGA database, characteristics analyzed based high- -throughput...
To investigate the expression of glutathione peroxidase 2 (GPX2) in human lung adenocarcinoma tissues and its effect on biological function A549 cells. The GPX2 survival were analyzed by TCGA database GEPIA database. A total 45 cases primary tissue specimens their paracancerous collected, two types was detected immunohistochemistry. Lung cells divided into overexpression group (GPX2), knockdown (si-GPX2), empty vector (Vector), siRNA negative control (si-NC), WT group; mRNA level protein...
Peroxiredoxins (PRDX6) regulates the occurrence and progression of cancer. The aim this study is to investigate effect PRDX6 knockdown on biological behavior human gastric cancer cell line BGC-823 cells.Research article.The differential expression in normal tissues was tested by immunohistochemistry. Ribonucleic acid plasmid gene packaged using a lentivirus, cells were transfected with lentivirus obtain which stably silenced.The proliferation activity detected counting kit-8 method....
Ginsenosides have notable bioactivity in treating cardiovascular diseases, but the mechanisms of their combined use with Peroxiredoxin 6 (PRDX6) myocardial injury remain unclear. This study explores synergistic effects Ginsenoside Rb1 (Gs-Rb1) and PRDX6, aiming to provide a theoretical foundation for therapeutic potential. We established rat model isoproterenol (ISO)-induced observed that combination therapy was more effective than single-drug treatments, as shown by ECG monitoring Masson...
Breast cancer remains a leading cause of mortality among women worldwide, emphasizing the urgent need for innovative prognostic tools to improve treatment strategies. Anoikis, form programmed cell death critical in preventing metastasis, plays pivotal role breast progression.
To construct a hp0532 gene-deleted mutant of Helicobacter pylori and study its impact on translocation CagA protein. A recombinant fragment containing kanamycin resistance gene that was flanked by the up- down-stream portions amplified engineered into pBluescript SK II-plasmid. suicide plasmidpBlueKM40-Δhp0532 based allelic exchange, Electrotrans formation H cells with pBshp0532-mutant resulted in isolation kanamycinresistant transformants, which identified polymerase chain reaction...
Abstract Objective The present study was undertaken to investigate the genetic diversity of Rumex patientia L. in distinct habitat types. Methods Eighty samples from 4 natural populations Jilin Province China were analyzed by inter-simple sequence repeats (ISSR). Results ISSR techniques produced 135 bands and 71 polymorphic bands, which (PPB) accounted for 52.6% among populations; gene (H) 0.1751, Shannon's index (I) 0.2634. In population, percentage loci (P) 25.93% 37.04%, 0.0714–0.1124...
Abstract Objective To investigate the effects of combined RNAi targeting human survivin with hypoxia-inducible factor 1α on gastric cancer BGC-823 cells. Methods The hypoxia sensitive cell line was identified firstly. Under condition, siRNA or/and HIF-1α were transfected into expression and assessed by RT-PCR Western blotting assay respectively. Cell apoptosis determined flow cytometry. proliferation measured MTT assay. abilities invasion migration evaluated transwell assays wound healing...
Abstract Objective: To investigate the effect of RNAi-mediated survivin and Hypoxia-inducible factor 1α (HIF-1α) gene silencing on proliferation apoptosis gastric cancer BGC-823 cells. Methods: Small interfering RNAs (siRNAs) targeting HIF1α mRNAs were designed synthesized, respectively, while scrambled siRNAs (SCRs) synthesized. The hypoxia sensitive line was identified transfected by Hifectin II in vitro under condition. cells with siRNA-survivin, siRNA-HIF-1α SCR named as sis group, siH...
Objective: To investigate the effect of RNAi-mediated survivin and Hypoxia-inducible factor 1α (HIF-1α) gene silencing on proliferation apoptosis gastric cancer BGC-823 cells. Methods: Small interfering RNAs (siRNAs) targeting HIF1α mRNAs were designed synthesized, respectively, while scrambled siRNAs (SCRs) synthesized. The hypoxia sensitive line was identified transfected by Hifectin II in vitro under condition. cells with siRNA-survivin, siRNA-HIF-1α SCR named as sis group, siH group...