A5011878659- Reproductive biology and impacts on aquatic species
- Effects and risks of endocrine disrupting chemicals
- Physiological and biochemical adaptations
- Zebrafish Biomedical Research Applications
- Adipose Tissue and Metabolism
- Birth, Development, and Health
- Lipid metabolism and biosynthesis
- Toxic Organic Pollutants Impact
- Neurogenesis and neuroplasticity mechanisms
- Aquaculture Nutrition and Growth
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Congenital heart defects research
- Fatty Acid Research and Health
- Lipoproteins and Cardiovascular Health
- Estrogen and related hormone effects
- Hypothalamic control of reproductive hormones
- Diet, Metabolism, and Disease
- Ovarian function and disorders
- Cancer, Lipids, and Metabolism
- Anesthesia and Neurotoxicity Research
- Epigenetics and DNA Methylation
- Reproductive System and Pregnancy
- Endoplasmic Reticulum Stress and Disease
- Liver Disease Diagnosis and Treatment
- Immune cells in cancer
University of Calgary
2016-2024
Alberta Children's Hospital
2016-2021
University of Waterloo
2012-2016
St. Michael's Hospital
2014-2016
University of Toronto
2015
St Michaels Hospital
2014
Glucocorticoid receptor (GR) signaling is thought to play a key role in embryogenesis, but its specific developmental effects remain unclear. Cortisol the primary ligand for GR activation teleosts, and zebrafish (Danio rerio), prehatch embryo content of this steroid maternal origin. Using early stages, we tested hypothesis that critical growth hatching. In zebrafish, mRNA degraded quickly, followed by zygotic synthesis receptor. protein widely expressed throughout development, were able...
Abstract In zebrafish ( Danio rerio ), de novo synthesis of cortisol in response to stressor exposure commences only after hatch. Maternally deposited is present during embryogenesis, but a role for this steroid early development unclear. We tested the hypothesis that maternal essential proper hypothalamus-pituitary-interrenal (HPI) axis activity and onset stressor-induced larval zebrafish. study, zygotic content was manipulated by microinjecting antibody sequester steroid, thereby making it...
Fetal BPA exposure, even below safe regulatory limits, disrupts the developing hypothalamus and affects behavior activity.
Abstract Background Although historically microglia were thought to be immature in the fetal brain, evidence of purposeful interactions between these immune cells and nearby neural progenitors is becoming established. Here, we examined influence embryonic on gliogenesis within developing tuberal hypothalamus, a region later important for energy balance, reproduction, thermoregulation. Methods We used immunohistochemistry quantify location numbers glial brain (E13.5–E17.5), as well...
Cortisol is the primary corticosteroid in teleosts that released response to stressor activation of hypothalamus-pituitary-interrenal axis. The target tissue action this hormone primarily mediated by intracellular glucocorticoid receptor (GR), a ligand-bound transcription factor. In developing zebrafish (Danio rerio) embryos, GR transcripts and cortisol are maternally deposited into oocyte prior fertilization influence early embryogenesis. To better understand molecular mechanisms involved,...
Abstract The hypothalamus is a key homeostatic brain region and the primary effector of neuroendocrine signaling. Recent studies show that early embryonic developmental disruption this can lead to conditions later in life, suggesting hypothalamic progenitors might be sensitive exogenous challenges. To study behavior neural progenitors, we developed novel dissection methodology isolate murine stem progenitor cells at timepoint day 12.5, which coincides with peak neurogenesis. Additionally,...
Our laboratory previously reported that lecithin:cholesterol acyltransferase (LCAT) and LDL receptor double knock-out mice (Ldlr(-/-)xLcat(-/-) or DKO) spontaneously develop functioning ectopic brown adipose tissue (BAT) in skeletal muscle, putatively contributing to protection from the diet-induced obesity phenotype. Here we further investigated their developmental origin mechanistic role of LCAT deficiency. Gene profiling muscle DKO newborns adults revealed a classical lineage. Primary...
Abstract Regulation of physiological homeostasis, including energy balance, is thought to be modified by low levels adult neurogenesis in the hypothalamus. Hormones such as oestradiol can influence both embryonic and hypothalamic neurogenic programs, demonstrating a sensitivity neural progenitor cells endogenous hormones. Previously we showed that gestational exposure environmental xenoestrogen bisphenol A (BPA) changed cell behaviors embryo; however, did not examine if these changes were...
Abstract Regulation of physiological homeostasis by the hypothalamus is influenced low basal levels adult neurogenesis arising from proliferative cell populations in various hypothalamic regions. Adult involved maintenance energy and responsive to balance disruptions. Hormones such as oestradiol interact with disruptions alter rates prenatal neurogenesis, demonstrating sensitivity neural progenitor cells endogenous hormones. We previously showed that gestational exposure hormone mimic...