A5015274291- Neurogenesis and neuroplasticity mechanisms
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Effects and risks of endocrine disrupting chemicals
- Epigenetics and DNA Methylation
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Pancreatic function and diabetes
- Developmental Biology and Gene Regulation
- Birth, Development, and Health
- CRISPR and Genetic Engineering
- Neonatal and fetal brain pathology
- Stress Responses and Cortisol
- RNA Research and Splicing
- Circadian rhythm and melatonin
- Genetics and Neurodevelopmental Disorders
- Hedgehog Signaling Pathway Studies
- Regulation of Appetite and Obesity
- Hypothalamic control of reproductive hormones
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Epilepsy research and treatment
- Immune cells in cancer
- Reproductive System and Pregnancy
- Toxic Organic Pollutants Impact
Alberta Children's Hospital
2016-2025
University of Calgary
2016-2025
Allen Institute for Brain Science
2014-2024
Alberta Children's Hospital Research Institute
2012-2023
Ontario Brain Institute
2022
Stichting Epilepsie Instellingen Nederland
2017
KU Leuven
2017
National Institute of Neurological Disorders and Stroke
2017
National Institutes of Health
2017
Neuroscience Institute
2017
Bisphenol A (BPA), a ubiquitous endocrine disruptor that is present in many household products, has been linked to obesity, cancer, and, most relevant here, childhood neurological disorders such as anxiety and hyperactivity. However, how BPA exposure translates into these neurodevelopmental remains poorly understood. Here, we used zebrafish link mechanistically disease etiology. Strikingly, treatment of embryonic with very low-dose (0.0068 μM, 1,000-fold lower than the accepted human daily...
Glyphosate is the most applied agricultural chemical worldwide and has become nearly ubiquitous throughout environment. an effective herbicide because it disrupts shikimate pathway, which responsible for synthesis of essential amino acids in plants microorganisms. Given that there no known target glyphosate higher animals, its toxicity to humans other animals heavily debated, especially after 2015 IARC ruling carcinogenic. Today, a growing body literature shows vitro, vivo, epidemiological...
The ventromedial hypothalamus (VMH) is a distinct morphological nucleus involved in feeding, fear, thermoregulation, and sexual activity. It essentially unknown how VMH circuits underlying these innate responses develop, part because the remains poorly defined at cellular molecular level. Specifically, there paucity of cell-type-specific genetic markers with which to identify neuronal subgroups manipulate development signaling vivo . Using gene profiling, we now ∼200 genes highly enriched...
Neural cell fate specification is well understood in the embryonic cerebral cortex, where proneural genes Neurog2 and Ascl1 are key determinants. What less how cellular diversity generated brain tumors. Gliomas glioneuronal tumors, which often localized cerebrum, both characterized by a neoplastic glial component, but tumors also have an intermixed neuronal component. A core abnormality tumor groups overactive RAS/ERK signaling, pro-proliferative signal whose contributions to differentiation...
Excess glucocorticoid transferred from stressed mother to the embryo affects developing vertebrate offspring, but underlying programming events are unclear. In this study, we tested hypothesis that increased zygotic deposition, mimicking a maternal stress scenario, modifies early brain development and larval behaviour in zebrafish (Danio rerio). Cortisol was microinjected into yolk at one cell-stage, mimic transfer, larvae [96 hours post-fertilization (hpf)] displayed activity light...
The brain plays a central role in controlling energy, glucose, and lipid homeostasis, with specialized neurons within nuclei of the mediobasal hypothalamus, namely arcuate (ARC) ventromedial (VMH), tasked proper signal integration. Exactly how exquisite cytoarchitecture underlying circuitry becomes established these remains largely unknown, part because hypothalamic developmental programs are just beginning to be elucidated. Here, we demonstrate that Retina anterior neural fold homeobox (...
Abstract The ventromedial nucleus of the hypothalamus (VMH) influences a wide variety physiological responses. Here, using two distinct but complementary genetic tracing approaches in mice, we describe development VMH efferent projections, as marked by steroidogenic factor‐1 (SF‐1; NR5A1). SF‐1 neurons were visualized Tau‐green fluorescent protein (GFP) expressed from endogenous Sf‐1 locus ( TauGFP ) or crossing transgenic Sf1:Cre driver to GFP reporter strain Z/EG ). Strikingly, projections...
Despite the development of newer anti-seizure medications over past 50 years, 30-40% patients with epilepsy remain refractory to treatment. One explanation for this lack progress is that current screening process largely biased towards transmembrane channels and receptors, ignores intracellular proteins enzymes might serve as efficacious molecular targets. Here, we report a novel drug platform harnesses power zebrafish genetics combines it in vivo bioenergetics assays uncover therapeutic...
Fetal BPA exposure, even below safe regulatory limits, disrupts the developing hypothalamus and affects behavior activity.
The three-layered piriform cortex, an integral part of the olfactory system, processes odor information relayed by bulb mitral cells. Specifically, cell axons form lateral tract (LOT) targeting (lot) guidepost cells in cortex. While lot and other cortical neurons share a pallial origin, factors that specify their precise phenotypes are poorly understood. Here we show mouse, proneural genes Neurog1 Neurog2 coexpressed ventral pallium, progenitor pool first gives rise to Cajal-Retzius (CR)...
G protein-coupled receptor (GPCR) pathways control glucose and fatty acid metabolism the onset of obesity diabetes. Regulators protein signaling (RGS) are GTPase-activating proteins (GAPs) for G(i) G(q) α-subunits that intensity duration GPCR signaling. Herein we determined role Rgs16 in regulation liver metabolism. is expressed during last few hours daily fast periportal hepatocytes, oxygen-rich zone where lipolysis gluconeogenesis predominate. knock-out mice had elevated expression...
Progenitor cells undergo a series of stable identity transitions on their way to becoming fully differentiated with unique identities. Each cellular transition requires that new sets genes are expressed, while alternative genetic programs concurrently repressed. Here, we investigated how the proneural gene Neurog2 simultaneously activates and represses expression in developing neocortex. By comparing activities transcriptional activator (Neurog2-VP16) repressor (Neurog2-EnR) fusions...
Proneural genes encode basic helix-loop-helix transcription factors that specify distinct neuronal identities in different regions of the nervous system. In embryonic telencephalon, proneural Neurog1 and Neurog2 a dorsal regional identity glutamatergic projection neuron phenotype presumptive neocortex, but their roles cell fate specification olfactory bulb, which is also partly derived from telencephalic progenitors, have yet to be assessed. Given bulb development guided by interactions with...
Significance Visceral pain is a debilitating type of that afflicts adults and children. Major challenges exist in developing analgesics due to the limited understanding mechanisms mediate visceral nociception. Using model colitis, we have identified granulocyte-colony–stimulating factor (G-CSF) as an essential mediator central sensitization leads hypersensitivity, even after resolution inflammation. We demonstrate G-CSF acting on spinal microglia activates signaling platform causes...
Abstract Background Although historically microglia were thought to be immature in the fetal brain, evidence of purposeful interactions between these immune cells and nearby neural progenitors is becoming established. Here, we examined influence embryonic on gliogenesis within developing tuberal hypothalamus, a region later important for energy balance, reproduction, thermoregulation. Methods We used immunohistochemistry quantify location numbers glial brain (E13.5–E17.5), as well...